Short term: 4°C; Long term: see manual. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
Adhesive Plate Sealers
Coated 96-well Strip Plate
Detection Antibody Diluent
Biotinylated Detection Antibody (100x)
HRP-Streptavidin Conjugate (100x)
HRP-Streptavidin Conjugate Diluent
Sample Diluent
TMB Substrate
Stop Solution
Wash Buffer (25x)
Standard (Lyophilized)
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CGRP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CGRP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CGRP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CGRP can be calculated. Restrictions
For research use only. Guarantee
This elisa kit carries the LSBio Guarantee. Top of PagePublications (0)Submit a Publication Top of PageCustomer Reviews (0)Submit a Review Top of PageImages
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