BHK-21细胞 叙利亚仓鼠肾细胞系更多培养经验分享

BHK-21细胞 叙利亚仓鼠肾细胞系更多培养经验分享 货号：GD-C19919171

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞 全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞 其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2'-Fluoro-4'-hydroxyacetophenone, 97%
1,2,3,5-Tetra-O-acetyl-β-D-ribofuranose
2-Fluoro-6-(trifluoroMethyl)pyridine-3-boronic acid
3-Butoxy-2,4,6-trifluorophenylboronic acid
SodiuM tetrakis(4-fluorophenyl)borate dihydrate
2-(4-Pyridyl)-4-Methylthiazole-5-carboxylic acid
10-PHENYL-1-DECANOL
5-Bromo-4-chloro-3-indolyl beta-D-cellobioside
Didodecyl Sulfoxide
2-bromo-3-methoxy-6-methylpyridine(SALTDATA: FREE)
(1S,2S)-N,N'-Bis[(R)-2-hydroxy-2'-phenyl-1,1'-binaphthyl-3-ylmethylene]-1,2-diphenylethylenediaminato Manganese(III) Acetate
3,5-Dimethoxybenzyl alcohol
2,5-Dimethylbenzaldehyde
(3R)-(+)-1-BENZYL-3-(TERT-BUTOXYCARBONYLAMINO)PYRROLIDINE
HexabroMobenzene
2-ChlorobenzylaMine
1,5-Dioxepan-2-one
M-Xylylene Dicyanide
Tween? 40
SodiuM Cobaltinitrite, Powder, Reagent, ACS
2,6-Di-tert-butyl-4-Methylcyclohexanone (Mixture of isoMers)
1,3-Dimethoxy-2-propanol
3-methyl-1-indanone
2-Amino-5-bromopyridine
4-(Trifluoromethyl)biphenyl
2-Butoxyethyl acetate
2,5-Dichlorobenzyl bromide
2-AMino-Thiazole-4-CarboxylaMide
TitaniuM(IV) oxide bis(2,4-pentanedionate)
2-(4-Hydroxyphenyl)ethanol, 98%
4-BroMo-2-Methyl-6-nitroaniline
Diphenyl diselenide
2-Methyl-5-nitroanisole
Perfluorotetracosane
6-[(3-chloropropyl)aMino]-1,3-diMethyluracil
Diethyl 1,3,5-benzenetricarboxylate
3-Iodo-5-(trifluoromethyl)-2-pyridinylamine
2-Amino-4-methylthiophene-3-carboxamide , 97%
2-(4-Chloro-phenyl)-pyrrolidine
Tetrahydrofurfuryl Methacrylate (stabilized with MEHQ)
4-METHYL-3-NITROBENZENESULFONAMIDE
2-ACETAMIDO-2-DEOXY-ALPHA-D-GLUCOPYRANOSYL CHLORIDE 3,4,6-TRIACETATE
DL-2-Bromohexanoic acid
POTASSIUM INDIGOTRISULFONATE
RhodaMine 110
4-AMinoindole
IsopropylideneMalononitrile
4-Chloro-2-Methyl-6-(1H-pyrazol-1-yl)pyriMidine
2-Chloro-1,3-thiazole, 97%
2,6-DIBROMOQUINONE-4-CHLOROIMIDE
3-Methylindole
tetrachlorothiophene
2-phenylbenzyl bromide
L-thyroxine sodium salt
4-Chlorodehydromethyltestosterone
L-GLUCOSE
2-AMINO-4-(4-CHLOROPHENYL)THIAZOLE
cis-2,6-DiMethylpiperidine , 99%
2,4,6-TRIFLUOROPHENYLACETONITRILE
1-Benzyl-4-cyano-4-hydroxypiperidine, 98%
N-Aminomorpholine, 97%
Benzylidenemalononitrile, 98%
2-Amino-5-bromophenol 99%
1,1-DIMETHYL-2-SELENOUREA
Ranitidine hydrochloride
1,2-Octanediol
(TRIMETHYLSILYL)DIAZOMETHANE
6-BroMo-2-naphthol
1-Benzyl-1-phenylhydrazine
MethylaMinoforMyl chloride
AcetaMide, 99%, pure
4-Piperazin-1-yl-furo[3,2-c]pyridine, 95%
Methyl Arachidate
5-tert-Butyl-2-Methylbenzenethiol
4-octanol
(S)-N-FMOC-2-Bromophenylalanine
Emedastine Difumarate
stannous trifluoromethanesulfonate
AST ELISA Kit
Mouse THP (Tamm–Horsfall Glycoprotein) ELISA Kit
APOA5 ELISA Kit
Human AHRR ELISA Kit
Chicken Interleukin 3,IL-3 ELISA Kit
Rat α1-microglobulin,α1-MG ELISA Kit
GPX-8 ELISA Kit
MCP-2 ELISA Kit
Human ADAMTS-13/vWF-cp ELISA Kit
PDGF-BB ELISA Kit
m-TGE Broth
Lecithin Tween 80 Nutrient Agar Medium
BisMuth(III) isopropoxide
ErbiuM fluoride oxide, 99.99% (REO)
5-Benzyloxyindole
2-Fluoro-5-iodopyridine, 97%
1,3-Dimethoxy-2-propanol
3-(NAPHTHALEN-2-YL)PROPAN-1-AMINE
Ammonium Bisulfite
N-Cinnamoyl-β-hydroxy-4-methoxyphenyl-aethylamin

3-METHOXY-1,2-PROPANEDIOL
5-Bromo-5-nitro-1,3-dioxane
2,6-Pyridinedicarboxylic Acid MonoMethyl Ester
4-Methoxy-4'-nitrobenzophenone, 97%
4-Fluoro-2-nitroaniline, 98%
2'-BroMo-6'-fluoroacetophenone, 96%
1-Boc-azetidine-3-carboxylic acid
CIS-ACONITIC ANHYDRIDE
2,3,4,5,6-PENTAFLUOROBENZENEBORONIC ACID
Ethyl 1-trityl-1H-iMidazole-4-carboxylate
DBD-F [=4-(N,N-DiMethylaMinosulfonyl)-7-fluoro-2,1,3-benzoxadiazole] [for HPLC Labeling], 98%
2-(tert-ButoxyMethyl)phenylboronic acid
N-(4-Ethylbenzyl)-N-methylamine , 97%
D-alpha-Tocopherol
3-Methyl-1-butene (in cylinder without valve) [To use this product charged in cylinder, a valve is required which is sold separately (Product Code:V0030)]