PC-61细胞:小鼠杂交瘤细胞传代要注意什么 货号：GD-C17520056
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞:全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题：如不能在收到细胞后及时操作细胞:T25及离心管发货的细胞将可以消毒后在37度过夜，血清发货的细胞在室温下静置1-2天(注意不要放冰箱)，干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱，若干冰完全升华，请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境，同时使部分因运输导致脱落的细胞贴壁，此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞:可以收集上清后离心(1200rpm，5min)后，将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中，由于不断震荡及环境不适，可能导致细胞破碎死亡，从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下，平衡后，贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可；悬浮细胞可以离心(1000rpm，3min)收集细胞:并用PBS重悬后再离心一次，再用新的培养基重悬并接种细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-Chloro-N-(2-fluorobenzyl)pyridine-4-carboxaMide, 95%
trans-Vaccenic acid
1H,1H-PERFLUORO-1-DECANOL
Benzyl (2R,3S)-(-)-6-oxo-2,3-diphenyl-4-Morpholinecarboxylate
3-BroMobenzothiophene-2-carboxaldehyde
4-PHENYLAZO-1-NAPHTHYLAMINE
Bis(triMethylsiloxy)Methylsilane
Methyl 3-aminobenzo[b]thiophene-2-carboxylate
2,5-Dideoxy-2,5-imino-D-mannitol ,98%
1-(4-Bromo-2-methylphenyl)-2-thiourea
Ethyl 1-(ethoxymethyl)-1H-imidazole-4-carboxylate
2-AMINO-4,5,6,7-TETRAHYDRO-1-BENZOTHIOPHENE-3-CARBONITRILE
1,4-Bis(3-aminopropyl)
(5-TERT-BUTYL-6H-[1,3,4]THIADIAZIN-2-YL)HYDRAZINE
TRIPHENYLMETHYL MERCAPTAN
2-BroMo-5-Methoxybenzoic Acid
2,2-DibroMo-2-cyanoacetaMide
N-Cyclopropyl 5-broMonicotinaMide
ThiochroMan-4-one, 98%
Orange Oil, Florida Type, Coldpressed, FCC
4-HydrazinobenzenesulfonaMide Hydrochloride
galangin
trans-1,2-cyclopentanediol
1-bromo-4-ethynylbenzene
Penta-Chloro Thiophenol
3-NITRO-4-PIPERIDINOBENZALDEHYDE
1-BenzyliMidazole
Poly(3-octylthiophene-2,5-diyl), regiorandoM, low Metals
Ethyl 2-(ethoxycarbonylMethyl)pyrrole-3-carboxylate, 95%
MolybdenuM blanked staMping, 0.76 x 12.7 x 12.7MM (0.003 x 0.5 x 0.5in), 99% Min
4-Hexylbenzoic acid
Methyl 4-chlorocinnaMate, 99%
2-CHLOROTOLUENE-4-SULFONIC ACID SODIUM SALT
S-Benzyl-L-cysteinol
Boc-(S)-3-aMino-5-hexenoic acid
VINYL PIVALATE
Methyl 2-pyrrolecarboxylate
2-Chloro-1-isopropyl-1H-imidazole
Phenyldimethylacetoxysilane
1,5-Diphenyl-3-(4-chlorophenyl)formazan
4-[(2-CHLORO-6-FLUOROBENZYL)OXY]-3-METHOXYBENZALDEHYDE
KANAMYCIN SULFATE
TRIMETHYLSILYLMETHYL ACETATE
2,2-Dimethoxyethylamine
4-[4-(tert-Butoxycarbonyl)piperazino]benzoic acid
3-BroMophenanthrene
3-Chloro-4-propoxyphenylboronic acid
CalciuM hypochlorite, tech., granular, 65% available chlorine
(R)-2-AMinooctane, 95%, 98% ee
1-Methyl-1-[4-(diphenylphosphino)benzyl]pyrrolidiniuM BroMide
3-AMino-1,2,4-triazole-5-carboxylic Acid HeMihydrate
Cyclododecanone
3-Bromophenylboronic acid pinacol ester
1-Bromo-2-chloro-4-fluorobenzene
Sethoxydim
Quinhydrone
(?)-tert-Butyl (S)-2-hydroxybutyrate
N,N-Diethyl-p-phenylenediaMine sulfate
(S)-3,3,3-TRIFLUORO-2-HYDROXY-2-METHYLPROPIONIC ACID
5,6-Difluoro-1H-indole-2-carboxylic acid
4-Chloro-2,6-dimethylpyridine
p-Tolyl Isobutyrate
o-Phenetidine Hydrochloride
1,2,3-THIADIAZOLE-4-CARBALDEHYDE
2-Acetyl-2-thiazoline
3,3'-Dichlorobenzidine dihydrochloride
Reinecke salt
4,6-Dichloro-2-(Methylthio)pyriMidine
1,3-Bis(2,4,6-triMethylphenyl)iMidazoliniuM chloride
N-MethylacetoacetaMide (ca. 70% in Water)
(2-Methylindol-1-yl)acetic acid
Benzylurea, 93%
HEMOGLOBIN
N-tert-ButyldiethanolaMine
Guaiacwood Oil
phosphomycin disodium salt
5-Amino-2-methylindole
alpha-Cyano-o-tolunitrile
ANG1-7 ELISA Kit
Mouse Gamma-aminobutyric acid,GABA ELISA Kit
Human ADRP ELISA Kit
Human histatin-5 ELISA Kit
LZM ELISA Kit
NTX ELISA Kit
PTH ELISA Kit
Human HPR ELISA Kit
Human AIG1 ELISA Kit
AGEs ELISA Kit
Mould Broth Medium
Glycerol glycine agar (Okazaki and Okami, 1976)
Indole-3-carbonitrile, 98%
StrontiuM chroMate
5-AMinobenzothiazole, 96%
2-Methyl-2-butene
galangin
Copper Pyrophosphate
boc-L-phenylalanine 4-nitrophenyl ester
Dydrogesterone
2-FLUOROPHENETHYL ALCOHOL
4-Propylphenol
Ethyl 4-Chlorobenzoate
n-Heptane, 95%
2-(2-Fluorophenyl)ethanol, 99%
6'-Methoxy-2'-acetonaphthone
Chloroxuron
2,4-DICHLORO-5-METHYLBENZENESULFONYL CHLORIDE
2,4-Dibutoxyphenylboronic acid
TRANS-1-METHYL-4-CARBOXY-5-(3-PYRIDYL)-2-PYRROLIDINONE
DPBP-bidentate phosphine
Barium carbonate (99.999%-Ba)PURATREM
3-Piperidin-1-ylaniline , 95%
Aflatoxin G1, crystalline
3-Chloro-6-(1H-pyrazol-1-yl)pyridazine 98%
