HCMEC-D3细胞系—人脑微血管内皮细胞系全国 货号:GD-C16920521
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-(FurfurylaMinocarbonyl)benzeneboronic acid pinacol ester, 97%
trans-3,5-DifluorocinnaMic Acid
N-Methyl-1,3-propanesultaM
N,N-Diethyl 4-broMonaphthaMide
2,6-DIBROMO-4-CHLORO-3,5-DIMETHYLPHENOL
Dicyclohexyl phthalate
beta-Ionone, synthetic, 96%
2,5-Difluorophenyl isocyanate, 98%
Pravastatin Sodium
1,3,5,7-Tetramethyl-6-phenyl-2,4,8-trioxa-6-phosphaadamantane, 99%
Vanadyl phthalocyanine, pract., 85%
4-FLUORO-2-(TRIFLUOROMETHYL)BENZYL ALCOHOL
2-Bromo-6-nitrophenol
Diethyl succinate
4-Phenyl-1-butanol
2-AdaMantanone
1-(2,4-Dinitrophenyl)pyridiniuM Chloride
N-Pentyl 3-broMophenylsulfonaMide
Bicyclo[3.3.1]nonane-2,6-dione, 97%
TranexaMic Acid, EP
1-MethyliMidazole
2-oxo-4-phenylbutyric acid
3,3'-iminobis(N,N-dimethylpropylamine)
Divinyl Benzene
m-Anisidine
1,5,9-TRIAZACYCLOTRIDECANE TRIHYDROBROMIDE
1-ETHYL-1H-TETRAZOLE
Ethyl phenylacetate, 99%
4-Fluoro-3-(trifluoroMethyl)cinnaMic acid, 97+%
1-Propanol, 99+%
Rebaudioside A
N-Boc-3-aMinoMethylpyridine
N-tert-ButylisopropylaMine, 98%
N-MethyldiethanolaMine
Ethyl 2-(3-chlorophenyl)-2,2-difluoroacetate
HEXAAMMINENICKEL (II) IODIDE
2,5-Hexanedione ,97%
5-Bromo-2-methoxypyridine
3-(2-Thienyl)pyrazole
1,2-Bis(3-methylthiophen-2-yl)ethane-1,2-dione, 97%
1,4-Benzoquinone dioxime
4-Hydrazino-6-methylpyrimidin-2-amine
Hexyl benzoate
1-METHYL-1-CYCLOHEXANECARBOXYLIC ACID
DL-1-AMino-2-propanol
1,2-Bis(trichlorosilyl)ethane
2-(t-ButoxycarbonylaMino)pyridine-3-boronic acid
Adipic acid, 99%
Ethyl 7-nitroindole-2-carboxylate, 95%
4-tert-Butylanisole
4-Heptanone
pyrimidin-5-amine
(Ra)-1,1'-spirobiindane-7,7'-diol
3-chloro-N-methyl-5-(trifluoromethyl)pyridin-2-amine
3-(4-Methoxyphenoxy)benzaldehyde
Imidazole-2-carboxaldehyde
DysprosiuM chloride hexahydrate
Di-tert-Butyl Malonate
8-Quinolineboronic acid
Thieno[2,3-c]pyridine
Cyclopropyl methyl ketone
2-Chloro-4-(methylthio)pyrimidine
2-Deoxy-2-fluoro-D-galactose
4-Chlorosalicylic acid
Methyl 2,5-dihydroxybenzoate
8-(DIETHYLAMINO)OCTYL 3,4,5-TRIMETHOXYBENZOATE HYDROCHLORIDE
3-BroMopropanoic acid;3-BroMopropionic acid
1,2-DiaMinopropane-N,N,N',N'-tetraacetic acid
1-Ethyl-3-MethyliMidazoliuM Hydrogen Sulfate
n-Octanal DiMethyl Acetal
6-(MethylaMino)nicotinonitrile
2,6-DiMethoxyphenylboronic acid, 97%
STEARYL OLEATE
1,3-Dichloro-5,5-diMethylhydantoin
9-Phenyl-9-fluorenol
3-(Trifluoromethoxy)benzaldehyde
hexafluorotitanic acid
Magnesium chloride hexahydrate
GDF11 ELISA Kit
Mouse L-Selectin ELISA Kit
Human f-βhCG ELISA Kit
Human PLUNC ELISA Kit
Monkey ApoC3 (Apolipoprotein C3) ELISA Kit
γ-ABA ELISA Kit
Chicken carbonic anhydrase,CA ELISA Kit
PKC-α ELISA Kit
ADAMTS7 ELISA Kit
ADRP ELISA Kit
Peptone-Salt Solution
VRE Agar Base
VinyltriMethylsilane
Furan-2-boronic acid, 97%
5-(2-Chloro-5-nitrophenyl)-2-furoyl chloride, 95%
Methyl 2-iodobenzoate, 98%
2-oxo-4-phenylbutyric acid
Naphthalene, 1-methoxy-2-methyl- (6CI,7CI,8CI,9CI)
Acid Red 336
Ammonium formate
2,4-DIBROMO-3-PENTANONE
4-Methylbenzyl acetate
2-Fluoro-6-Nitro-PhenylaMine
PotassiuM chlorochroMate, 97+%
(S)-(+)-4-Penten-2-ol, 97%
2-Fluoroiodobenzene
α-Tetralone
3-(Morpholino)phenylboronic acid
o-Phenanthroline Monohydrochloride Monohydrate
PALMITIC ACID HYDRAZIDE
SodiuM Quinoline-8-sulfonate
2-(4-Methanesulfonamidophenyl)acetic acid
2,4-Dihydroxy-6-methylpyrimidine, 97%
Bromophenyltrichlorosilane (mixed isomers)
S-Phenyl Thioacetate