Super Tube细胞系—狗肾细胞系全国 货号:GD-C16919213
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3,5-Dihydroxybenzoic acid, 98%
Z-Gln-OH
2-Acetyl-3-thienylboronic acid
BANSYL CHLORIDE
1-BroMo-3-cyclopentenylbenzene
HEXAKIS(BROMOMETHYL)BENZENE
N-(4-METHYL-3-CHLOROPHENYL)MALEIMIDE
5-Chloro-2-methylbenzenethiol
5-(4-bromo-1H-pyrazol-1-yl)-1H-tetrazole(SALTDATA: FREE)
[(9H-Fluoren-9-ylmethoxycarbonyl)-methyl-amino]-acetic acid
Boc-S-trityl-L-homocysteine
Ethyl 4-aminophenylacetate
3-CHLORO-4-NITROBENZOTRIFLUORIDE
N-(tert-Butoxycarbonyl)-2-aminoacetonitrile
3-(ChloroMethyl)benzoyl chloride
Cyclopropanecarboxaldehyde
3,5-DibroModithieno[3,2-b:2',3'-d]thiophene
Methyl 5-broMo-2-chloroisonicotinate
Vinylidene chloride, 99.9%, stabilized
SodiuM Tartrate, Dihydrate, Reagent, ACS
4-Vinylbiphenyl
stearic anhydride
p-(phenylthio)benzyl alcohol
3-Methylxanthine
Benzoic acid, 3-fluoro-, methyl ester
COBALT (II) PERCHLORATE
4-Chloro-2-fluorobenzoic acid
3-Nitrophthalonitrile
2,6-DichlorodiphenylaMine, 98%
tert-Butyl nitrite, tech. 90%
PotassiuM D-gluconate
Acetophenone
Ethyl 2-nitrobuytrate
P-ANISALDEHYDE DIMETHYL ACETAL
3,5-DibroMobenzoic acid
gaMMa-Methyl-gaMMa-decanolactone , 97%
Calcium fluoride, anhydrous, pure, 99%
p-Toluenesulfonamide
Thioxanthen-9-one 97%
2-Methylisophthalonitrile
Olopatadine
Sodium xylenesulfonate
Disperse Blue 56
Cupric sulfate
Phthalazine
γ-Dodecalactone
Methyl Difluoroacetate
4(7)-NitrobenziMidazole
Methyl 3-Methyl-4-nitrobenzoate, 97%
Glyoxylic acid monohydrate
6-Methylpyridine-2-carboxylic Acid
Methoxydimethylphenyl Silane
Uranium nitrate;Uranyl nitrate
Isopropyl cyanoacetate
2-HEPTYLBENZIMIDAZOLE
2,4-Dichloro-5-fluorobenzoyl chloride
3,3',4,4'-DIPHENYLSULFONETETRACARBOXYLIC DIANHYDRIDE
CyclohexylMagnesiuM BroMide (ca. 17% in Tetrahydrofuran, ca. 1Mol/L)
4-AMINO-3-ETHYLBENZONITRILE
2-Iodophenol 98%
5-Amino-2-methoxypyridine-4-carboxylic acid ,97%
1-Benzyl-3-piperidone hydrate hydrochloride, 95%
3,4'-Dichloropropiophenone
METHYLMERCAPTO-D-GALACTOPYRANOSIDE
Benzamidine hydrochloride
GOLD
TETRAHYDRO-3-FUROIC ACID
3-Nitroaniline
1-BroMo-2-chloro-4-fluorobenzene
3,5-DiMethoxyphenylboronic acid, pinacol ester
Cyclopropanecarboxaldehyde, 98%
4-AMino-3-nitrobenzoic acid, 98%
3-(2-BenziMidazolyl)-7-(diethylaMino)couMarin
Pentaethylene Glycol
Trimethyltin chloride
N-(3,4-Dichloro-2-fluorophenyl)-6-methoxy-7-{[(3aR,6aS)-2-methyloctahydrocyclopenta[c]pyrrol-5-yl]methoxy}-4-quinazolinamine
Pyrazine,2-(1-methylpropyl)-
Quinapril hydrochloride
PLAP ELISA Kit
Mouse Slit2 ELISA Kit
Human PROG ELISA Kit
Human deubiquitinating enzyme,DUB ELISA Kit
IL-1 ELISA Kit
CRYaB ELISA Kit
Chicken TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit
Human CEPT1 ELISA Kit
HO1 ELISA Kit
IGF1 ELISA Kit
Soybean-Casein Digest Agar
Bacteria Save Medium,Semisolid
Isopropyl Acetoacetate
YTTRIUM FLUORIDE
Ethyl 5,6-diMethoxyindole-2-carboxylate, 98+%
2-Fluoro-4-nitrobenzyl alcohol, 97%
stearic anhydride
2-Trimethylsiloxypent-2-ene-4-one
2-Naphthyl octanoate
2,4-Pyridinedicarboxylic acid
DL-Pipecolinic acid
Zinc bacitracin
3-Chloro-5-Fluorophenylacetonitrile
2,3-Difluorobenzyl broMide, 97%
N-Boc-D-glutaMic acid 5-benzyl ester, 98%
2-Cyclopentyloxypyridine-5-carboxaldehyde, 98%
2-BroMopyridine-5-boronic acid pinacol ester
Lead distearate
1-BOC-7-broMoindole
2-Chloro-5-hydroxyMethylpyridine
5-BroMo-2-t-butylaMinopyriMidine
trans-2,4,5-TriMethoxycinnaMic acid
3-Bromo-6-fluoro-1H-indazole
D-Allothreonine
2-Bromo-5-fluorophenylacetonitrile 99%