PG49细胞系—人肺腺癌细胞系全国减价 货号：GD-C16819717
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
养了几年细胞系—说一点自己培养细胞方面的看法：1)培养细胞前，可以看看细胞特点说明书，包括细胞正常生长的状态图、传代、培养基的类型等；2)不同细胞生长周期不同，有的生长很快，比如说MDA-MB-231，传代的时候取离心悬液的十分之一就已经足够了，有的又是特别慢，等的人心焦，BT474就是，传代是一分二，复苏起始的时候两周多才能长满，所以就要在培养细胞的过程中多多摸索了；3)对于娇弱的细胞系—就得细心呵护了，胰酶消化不能过久，吹得时候要轻柔，离心时候也要注意转速和时间，每天都要去看一下细胞系—肉眼观察培养基状况、显微镜下观察细胞生长状况；4)冻存细胞复苏后第二天Z好更换一次培养基；5)培养箱隔一段时间彻底用酒精棉擦洗一次；6)养细胞的教训：不能偷懒!细胞虐我千百遍，我待细胞如初恋。1)不管买的细胞、惠赠的细胞系—刚拿到手赶紧做两件事：检测是否有支原体污染; 迅速扩增冻存；2)发现有污染迅速处理掉，特别是当你养了很多种细胞时; 细菌污染，真菌污染很容易发现，支原体污染的话，细胞会长的慢，买个支原体检测的kit定期检测一下；3)传代细胞日常的值日清洁是必须的，此外还要定期熏蒸；4)细胞的传代一定要规律，保持相同的confluency和传代时间间隔，不要随心所欲或者拖延；5)注意个人卫生。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
[Nle4, D-Phe7]-α-Melanocyte StiMulating HorMone trifluoroacetate salt
2-Hydroxy-5-nitronicotinic acid
3-(CyclopropylMethyl)thiophenylboronic acid
4-(2-(DiMethylaMino)ethoxy)phenylboronic acid, pinacol ester
4-Nitrobenzophenone
Perfluorooctyl iodide
6-Oxo-1,4,5,6-tetrahydropyridazine-3-carboxylic acid, 97%
Boc-Ser-OH
Triethyloxonium tetrafluoroborate, 95%
2-Ethyl-1-hexene
Dimethyl 2,6-Naphthalenedicarboxylate
TETRAFLUOROSUCCINIC ACID
L-Homophenylalanine hydrochloride
(+)-Dipentene
2,4,5-TrichloropyriMidine
2-Fluoro-4-methylbenzoic acid
N,N-DiMethyl-N-(MethylsulfanylMethylene)aMMoniuM Iodide
2,3-Difluoro-4-forMylphenylboronic acid
4-(4-Methoxyphenyl)pyriMidine-2-thiol, 95%
1,2-DICHLOROTETRAFLUOROETHANE
MethylaMinoacetonitrile Sulfate
Oxalyl dihydrazide
2-Acetoxy-3-methoxybenzaldehyde
4-Bromo-2-(trifluoromethyl)anisole
N-Acetyl-1-aminonaphthalene
PENTAFLUOROPHENACYL BROMIDE
Aminoacetonitrile hydrochloride
3,6-Dichloro-4-Methylpyridazine
2-Methyl-4-phenylthiazole, 97%
2,5-DiMethylbenzoic acid, 96%
2′-Deoxycytidine 5′-Monophosphate
2-Phenyl-2-propanol
ETHYL 5-CHLOROTHIOPHENE-2-CARBOXYLATE
N-Ethyl 3-broMo-4-fluorobenzaMide
tert-Butyl 2,2,2-trichloroacetiMidate
TERT-BUTYL ETHYL ETHER
Methyl 4-methoxythiophene-3-carboxylate , 97%
Potassium (3-cyanophenyl)trifluoroborate
5-Amino-4-cyano-3-(cyanomethyl)pyrazole
6-(Methylamino)nicotinonitrile
Tetrabutylammonium tetrafluoroborate
Gliclazide
2-Deoxy-2,2-difluoro-D-erythro-pentofuranose-3,5-dibenzoate-1-methanesulfonate
(R)-(+)-1,1'-Bi-2-naphthol
1-Hydroxybenzotriazole Monohydrate
N-Acetyl-2-oxindole
Tridecanal
1-Pentanol, 99%, pure
{2-[(4-Methylpiperazin-1-yl)Methyl]phenyl}MethylaMine, 97%
O-NITROPHENYL-1-THIO-BETA-D-GALACTOPYRANOSIDE
trans-3-Pentenoic Acid
3-Chloro-4-iodopyridine
2,5-Cyclohexadiene-1,4-dione,2-heptadecyl-6-methoxy-
2-Amino-5-chloropyrazine
arachidic acid ethyl ester
1,3-BENZOTHIAZOLE-2-CARBONYL CHLORIDE
DiMethyl oxalate
4-BroMo-6-chloro-2-(Methylthio)benzo[d]thiazole
5-(4-Chlorophenyl)-1,3,4-oxadiazole-2-thiol
3-Nitro-o-xylene, 97%
5-[4-(Bromomethyl)phenyl]-3-methyl-1,2,4-oxadiazole , 95%
5-formyl-2-methoxyphenyl acetate(SALTDATA: FREE)
tert-Butyl 2-Bromoisobutyrate
2-Naphthaleneboronic acid
ACETYLSALICYLIC ACID METHYL ESTER
VITAMIN A
1,1-DIMETHOXYCYCLOHEXANE
Methyl 2-Mercapto-1-Methyl-1H-iMidazole-5-carboxylate
1-Octyl-3-MethyliMidazoliuM BroMide
Diisodecyl 4-Cyclohexene-1,2-dicarboxylate
1-Pyrenebutyric acid, 97+%
Benzoyleneurea, 98%
Diphenyl oxide
5beta-Pregnane-3alpha,20alpha-diol
2H-3,1-Benzoxazine-2,4(1H)-dione,5-bromo-
terbinafine hydrochloride
cyclopropyldiphenylsulphonium tetrafluoroborate
Bis(trimethylsilyl)sulfate
HSP-90 ELISA Kit
Mouse FLI1 (Friend Leukemia Virus Integration 1) ELISA Kit
Human hnRNP I/PTB ELISA Kit
Human DRD3 ELISA Kit
Monkey TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit
Rat IL-12/p40 (Interleukin 12 p40) ELISA Kit
TRAIL-R3 ELISA Kit
Human PR3 ELISA Kit
Human PDGF-BB ELISA Kit
NK ELISA Kit
Skirrow Agar Base
Skirrow Agar Base
MagnesiuM acetate tetrahydrate, ACS, 98.0-102.0%
(Trans,Trans)-4-Pentyl-[1,1-Bicyclohexyl]-4-Carboxylic Acid
4-n-Butylbenzenesulfonyl chloride, 97%
2-PhenoxyethylbroMide
Oxalyl dihydrazide
Sodium Chloride
6-Bromo-O-Cresol
2,6-Dimethylnaphthalene
METHYLTRIPHENYLSILANE
4-TERT-BUTYL-3,5-DIMETHYL-1H-PYRROLE-2-CARBOXYLIC ACID ETHYL ESTER
Ethyl Tetrahydropyran-4-Carboxylate
3,4,6-Tri-O-Methyl-D-glucal, 97%
1,2-Propanediol, 98+%
2-Fluoro-4-(trifluoroMethyl)pyridine
3-ForMyl-pyrrolidine-1-carboxylic acid tert-butyl ester
SodiuM tert-pentoxide
3-Acetyl-2,4,6-triMethylpyridine
3-AMino-2-Methylphenol
2-Fluoro-3-pyridylboronic acid
1-(3,5-Dimethylphenyl)
2-Nitro-4-(trifluoromethyl)phenylacetic acid 98%
1-(4-pyridinyl)-N-(4-pyridinylmethyl)methanamine(SALTDATA: HCl)
3-Chlorothiobenzamide
