HT 1197细胞系—人膀胱癌细胞系全国减价 货号：GD-C16820586
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞系—将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-(TrifluoroMethyl)benzyl chloride, 98%
2-Isopropyl-5-Methyl-2-hexenal
TETRAPHENYLARSONIUM CHLORIDE HYDROCHLORIDE
4'-AMINOOCTANOPHENONE
1,1′-Binaphthyl-2,2′-diaMine
3-(DiMethylcarbaMoyl)-5-nitrophenylboronic acid
2-Hydroxy-5-nitropyridine
(pyrimidin-4-ylmethyl)amine(SALTDATA: 2HCl)
(S)-1-N-Boc--2-carboxylic acid
Copper(II) methoxide, 99%
5-methyl-1H-indazole(SALTDATA: FREE)
Ketoprofen
1-Iodopentane
Heptyl 4-hydroxybenzoate
DL-2,4-DIAMINOBUTYRIC ACID DIHYDROCHLORIDE
2-Thiopheneacetyl chloride
Difluoroacetaldehyde Ethyl HeMiacetal
(4-BroMopyridin-3-yl)Methanol
Sudan I, pure
MES, Monohydrate, Ultrapure
2-(BroMoMethyl)-4,4,5,5-tetraMethyl-1,3,2-dioxaborolane
Drostanolone Enanthate
acid violet 7
1-Chloroheptane
2-Methylundecanal
Sodium hydride
SODIUM METHACRYLATE
1,4-Dihydro-2,6-DiMethyl-3,5-Pyridinedicarboxylic Acid Diethyl Ester
4-Fluoronaphthalene-1-boronic acid, 98%
Nitroterephthalic acid, 99%
Astragaloside III
YttriuM nitrate hexahydrate
METHYL 4-(AMINOMETHYL)BENZOATE HYDROCHLORIDE
Terpineol
2′-AMinoacetophenone hydrochloride
2-PYRIDYLDITHIOCARBAMIC ACID ETHYL ESTER
2,4-Difluorophenyl isocyanate, 99%
4-Amino-2,6-dimethylpyrimidine
2-Bromoethyl methyl ether
o-Methylvalerolactim
L-Tyrosine
EUGENOL ACETATE
carbazol-4-one hydrochloride
(+/-)-trans-1,2-Diaminocyclohexane
Oleic acid
2-Naphthyl phosphate sodiuM salt
3,5,4'-TribroMosalicylanilide
1-Nitroso-2-naphthol-3,6-disulfonic acid, disodiuM salt hydrate, pure, indicator grade
3,5-Dichlorobenzene-1-carbohydrazide, 97%
8,8'-DIQUINOLYL DISULFIDE
Dibenzyl Phosphate
(3S,8S,9S,10R,13R,17R)-17-[(1R,4R)-4-ethyl-1,5-dimethyl-hexy...
5-Bromo-2-hydroxy-3-(trifluoromethyl)pyridine
2,4-dichlorobiphenyl
3-acetoxypyridine
CHROMONE-3-CARBOXYLIC ACID
GadoliniuM rod, 6.35MM (0.25in) dia, 99.9% (Metals basis excluding Ta)
N-METHYLSUCCINIMIDE
DexaMethasone
Diethyl(3-pyridyl)borane
3-Ethoxyphenethylamine
5-Formylthiophene-3-boronic acid
6-Chloro-1,2,3,4-tetrahydroquinoline
4-Carboxylphenylboronic acid pinacol ester
2-Acetyl-1-methylpyrrole
TEMAZEPAM
2,3-DiMethyl-1,3-butadiene;2,3-DiMethylerythrene;Diisopropenyl;β-γ-DiMethyl-Δ-α-γ-butadiene;
3-Methyl-5-(5-Methylisoxazol-3-yl)isoxazole-4-carboxylic acid
1-(4-BroMophenyl) Hydrochloride
6-Ethyl-1,6-dihydropyriMidin-5-ylboronic acid
Dichlorophenylborane, 97%
3-aMino-5-hydroxybenzoic acid hydrochloride hydrate, 95%
2-(4-DiethylaMino-2-hydroxybenzoyl)benzoic Acid
2,6-DiMethylbenzyl Chloride
Chlorocarbonyl ferrocene
tert-butyl 1,8-diazaspiro[5.5]undecane-1-carboxylate
3-CHLORO-5-FORMYL-2-METHOXYPYRIDINE
2,4-difluorobenzamide
OxLDL ELISA Kit
IL2 ELISA Kit
Human TNF-α ELISA Kit
Human DAG ELISA Kit
α1M ELISA Kit
TBAM ELISA Kit
Monkey Interleukin 6,IL-6 ELISA Kit
Human Brucella Ab IgG ELISA Kit
H-FABP ELISA Kit
GDF8 ELISA Kit
Muller–Kauffmann Tetrathionate Broth Base
BBL Agar Medium
Guaiazulene, 98+%
D-Fructose-1,6-diphoshate calcium salt
5-Cyclobutyl-5-phenylhydantoin, 97+%
2-MethyliMidazo[4,5-c]pyridine, 99%
Drostanolone Enanthate
Selenium
(6aR,12aR)-6a,12a-dihydro-6H-[1,3]dioxolo[5,6][1]benzofuro[3,2-c]chromen-3-yl beta-D-glucopyranoside
Benzoin
1H,1H,5H-OCTAFLUOROPENTYL ACRYLATE
4-Chloro-isatoic anhydride
3,4-Dihydroxybenzophenone, 98%
AluMinuM antiMonide, 99.99% (Metals basis)
3-AMidinopyridine hydrochloride, 97%
2'-DeoxythyMidine-5'-diphosphate trisodiuM salt
2-(ChloroMethyl)-5-nitrophenylboronic acid
7-Chloroquinoline-4-boronic acid
Oxacyclododecan-2-one
3-Iodobenzyl broMide
3-Methylbenzoyl chloride
Tribromoacetic acid, 99%
Methanol-d, 99.0 atom % D, for NMR
octahydropyrrolo[1,2-a]pyrazine(SALTDATA: FREE)
2-(4-Bromophenyl)benzothiazole
