3T3swiss细胞系—小鼠胚胎成纤维细胞系全国减价 货号:GD-C16820502
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Methyl Purple, 0.1% w/v aq. soln.
Phenylphosphine
4-Methoxybenzyl 5-broMo-2-chlorobenzaMide
3-ACETOXYBENZALDEHYDE
MTT FORMAZAN
4-CHLOROBENZYLIDENEACETONE
4-(4-Nitrophenylazo)catechol, 98%
2-Chloro-N-[4-chloro-2-(2-chlorobenzoyl)phenyl]acetamide, 97%
Lauric Anhydride
Maleic acid, 99%
2-Amino-4-hydrazino-6-methylpyrimidine ,97%
N-(2,3-Dihydro-2-oxo-1H-benzimidazol-5-yl)-3-hydroxy-2-naphthalenecarboxamide
5-CHLORO-2,3-DIBROMO-1-FLUOROBENZENE
Sodium selenite
TetraMethylaMMoniuM Chloride
Chlorophenol Red
Donepezil Hydrochloride
Methyl 3-broMo-5-(trifluoroMethyl)benzoate
3-Chloropyridine-2-carboxylic acid, 97%
ThyMe Oil, FCC
3-Methoxythiophene
3,5-Dimethoxybenzyl alcohol
Pentaerythritol tetrakis[3-(3',5'-di-tert-butyl-4'-hydroxyphenyl)propionate]
cristobalite
n,n,n-trimethyl-1-hexadecanaminiuhydroxide
1-Indanamine
6-Benzyloxyindole-3-carboxaldehyde
GlybenzcyclaMide, 99%
4-Iodo-L-phenylalanine, 95%
3-BroMo-2-chloro-5-(trifluoroMethyl)pyridine, 97%
L-3-Cyanophenylalanine
PRASEODYMIUM CHLORIDE
(+)-Bis[(R)-1-phenylethyl]aMine
N-OCTANAMIDE
4-Chloro-2,6-difluorophenylaceticacid
2-Fluorobenzenesulfonyl chloride
5-(Hydroxymethyl)uracil ,98%
3-(4-Methyl-piperazin-1-yl)-propionic acid ethylester
Sucrose monocaprate ,98%
4-Cyanobenzyl-triphenylphosphonium chloride, 98%
3-BROMO-5-FLUOROBENZALDEHYDE
4-CHLORO-2-FLUOROIODOBENZENE
CALCIUM GLUCONATE MONOHYDRATE
2,6-Difluorobenzaldehyde
Methyl sulfone
1,3-Bis(4-Methoxyphenoxy)benzene
3-Butoxy-2,4,6-trifluorophenylboronic acid
alpha-Chloro-p-xylene, 99+%
8-AMinoisoquinoline, 97%
Acid Red 114
RutheniuM(III) Chloride
2-Chloroquinoline
(R)-N-BOC-2-(5-Bromothienyl)alanine
Amines, C20-22-alkyldimethyl
1-(4-Fluorophenyl)-1,3-dihydro-isobenzofuran-5-carbonitrile
3-Bromo-1,1,1-trifluoroacetone
2-Decyl-1-tetradecanol
RutheniuM(III) chloride trihydrate
2-AMino-4-chlorobenzoic acid
1-Phenacylpyridinium Bromide
o-Tolylhydrazine Hydrochloride
Isophorone
3-Phenylpropyl Chloride
2-(METHYLTHIO)THIOPHENE
Ethyl benzenesulphonate
1,3,5-TRIETHYNYLBENZENE
2-CHLORO-5-(TRIFLUOROMETHYL)BENZONITRILE
Acetanilide
1-Cyclohexyl-1-pentanol
3-Propoxyphenylboronic acid
Hydriodic acid, ACS reagent, 55% in water, without stabilizer
Methyl 2-(hydroxyMethyl)acrylate, 93%, stabilized with 0.1 wt.% MEHQ
3,5-Dichlorophenol
1,3-Dibutylthiourea
N-Methylcaprolactam
2,6-Dichloroquinone-4-chloroimide
H-D-Tyr(tBu)-OH
Angoroside C
Porcine Growth Hormone Releasing Peptide,GHRP ELISA Kit
NT-proBNP ELISA Kit
FPSA ELISA Kit
Human OC90 ELISA Kit
C4 ELISA Kit
BTC ELISA Kit
Chicken NT-3 (Neurotrophin-3) ELISA Kit
Human PKB ELISA Kit
Human THBS1 ELISA Kit
sPs ELISA Kit
Ethyl Violet Azide Broth
Pfizer Enterococcus Selective Agar
Diethyl acetaMidoMalonate, 98+%
MagnesiuM Trisilicate
5-(2-NITROPHENYL)-2-FURALDEHYDE
2-IodoiMidazole, 96%
3,5-Dimethoxybenzyl alcohol
diethyl chloromalonate
Solvent Black 27
Ethyl n-Valerate
Yttrium oxide
Tin Tetrachloride
2-Picoline, 98+%
TetraphenylphosphoniuM broMide, 98+%
4-tert-Butyl-2,6-dinitroanisole, 98%
Furazolidone
2-Thiopheneacetic acid
(R)-(+)-1-(3-Methoxyphenyl)ethyl isocyanate, 97%
4-BroMo-7-hydroxy-1-indanone
Propynol ethoxylate
N-NONADECANOPHENONE
3-(Trifluoromethoxy)benzyl chloride
Pentafluorobenzenethiol
1-(2-Hydroxyethyl)imidazolidine-2-thione
Dimethyl (R)-(+)-Methylsuccinate