3T3NIH细胞系—小鼠胚胎成纤维细胞系全国减价 货号:GD-C16820501
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Molecular sieves, titaniuM oxide based; Mesoporous, 25 AngstroM, powder
4-BroMobutyryl chloride
ChloroMethyl isopropyl carbonate
4-BroMo-3-fluorobenzenesulfonyl chloride
Methyl 2-phenylsulfinylacetate
CesiuM forMate
1-AMINO-4-BROMOANTHRAQUINONE-2-SULFONIC ACID SODIUM SALT
(3,5-dimethyl-1H-pyrazol-1-yl)acetic acid(SALTDATA: FREE)
Caspase-2 Inhibitor (Aldehyde)
Dichloromethylsulphonyl chloride
Methyltris(dimethylsiloxy)silane
YTTRIUM FLUORIDE
1,4-DIOXANE-D8
2-Ethylpyridine
4-(3-Thienyl)benzoic acid
CyclohexylaMine hydrochloride
6,7-DiMethoxyisoquinoline
4-BroMo-2-nitrobenzaldehyde
1-Benzofuran-3-ylacetonitrile, 97%
Thiosalicylic Acid
4-Chloroanisole
2-Nitro-1-phenylpropene
(6aS)-1,11-dihydroxy-2,10-dimethoxy-6,6-dimethyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinolinium chloride
Cobalt(II) sulfide
dipropoxymethane
2,3,6-TRICHLOROBENZYL BROMIDE
1,3-DIACETOXY-1,1,3,3-TETRABUTYLDISTANNOXANE
2-BroMohexanoic Acid
4-(2-AMinoethyl)pyridine, 97%
3-Fluoro-o-xylene, 99%
Glycyl-L-serine
Methyl 3-oxovalerate
MAGNESIUM SALICYLATE
N-OCTANOIC ACID P-TOLYL ESTER
2-Fluoro-5-isobutoxyphenylboronic acid
TMPYP
Pentafluorobenzamide 99%
5-Formyl-2-methoxypyrimidine
Bis(chloromethyl)dimethylsilane
4,5-Dimethoxy-2-nitrobenzyl carbonochloridate
(1R,2S,5R)-5-Methyl-2-(1-methylethyl)cyclohexyl dihydroxy-acetate
(-)-ALPHA-BISABOLOL
2-[4-(Dibutylamino)-2-hydroxybenzoyl]benzoic acid
Tetramethylpyrazine
AntiMony trichloride
1-(tert-Butoxycarbonyl)-3-piperidinecarboxylic acid
1-[[(9H-Fluoren-9-ylMethoxy)carbonyl]aMino]cyclopentanecarboxylic Acid
5-AMinoMethyl-2-fluoroanisole
2,2'-DiMethoxy-1,1'-binaphthyl, 98%
Chlorotributyltin
BenzyltriMethylaMMoniuM Tetrachloroiodate [Chlorinating Reagent]
propargite
Tungsten(VI) chloride
midodrine hydrochloride
Cyproheptadine hydrochloride
2-Ethyl-1,3-cyclopentanedione
11,11,12,12-TETRACYANONAPHTHO-2,6-QUINODIMETHANE
3-(3-Nitrophenyl)-5-(thiophen-2-yl)-1,2,4-oxadiazole
Methyl Red sodiuM salt
Trifluoroacetaldehyde ethyl hemiacetal, tech.
3'-Methoxy-2,2,2-trifluoroacetophenone
C-Phenyl-C-pyridin-4-yl-methylamine
(1-isopropyl-1H-imidazol-2-yl)methanol(SALTDATA: FREE)
2-Bromofluorobenzene
3-(4-FLUOROPHENYL)BENZALDEHYDE
DL-2-Phenylpropionic acid
2,4-Difluoroaniline
4-Isopropylphenylboronic Acid (contains varying aMounts of Anhydride)
1-Cyclohexyl-1-butanol
2-Propoxyphenylboronic acid
Hydrindantin dihydrate, 96%
6-Methoxy-2-naphthylMagnesiuM broMide, 0.5M solution in THF, AcroSeal
Dehydrocholic Acid
6-BroMo-2-hydroxy-3-Methoxybenzaldehyde
Calcium methoxide
Triallyl cyanurate
nonafluorohexyltris(dimethylsmino)silane
Fenoxanil
Porcine Growth Hormone Releasing Peptide,GHRP ELISA Kit
NT-proBNP ELISA Kit
Human fPSA ELISA Kit
Human CTBS ELISA Kit
LN ELISA Kit
Rat beta cellulin,BTC ELISA Kit
NT3 ELISA Kit
Human protein kinase B,PKB ELISA Kit
Human PECAM-1/CD31 ELISA Kit
OPM ELISA Kit
Dextrose Broth
Pfizer Enterococcus Selective Agar
Acetamide
FUMONISIN B1
5-(2-Nitrophenyl)-2-furoyl chloride
2-IodopyriMidine
2-Nitro-1-phenylpropene
Ethyl (3-bromophenyl)acetate
Reactive Red 31
Ethyl 3-bromopropionate
2,6-Dihydroxybenzoic acid
TRICYCLOHEXYLTIN HYDRIDE
4-Vinylbenzenesulfonic Acid SodiuM Salt Hydrate
2-(3-Methylphenoxy)ethanol, 98%
EthyltriphenylphosphoniuM iodide, 98+%
3-Pyridinecarboxaldehyde
3-Methyl-1-indanone
2-BROMO-2-BUTENE
1-Chlorotetradecane
p-Anisohydrazide
Ethyl picolinate
5-Nitro-2-phenylpyridine
Boc-4-aminobenzoic acid
3,5-Dimethyl-4-(2-iodooethyl)isoxazole
Ethyl 2-aminoisonicotinate 97%