HCT-15[HCT15]细胞系—人结直肠腺癌细胞系全国减价 货号:GD-C16819042
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞系—为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2,4-Di-tert-butylphenol, 97%
Trypsin Inhibitor Soybean
TRIETHYLENE GLYCOL DIMETHYL ETHER
4-Fluoro-2-Methylbenzenesulfonyl chloride, 97%
N,N-DiMethylforMaMide tris(3-cyano-5-nitrophenylboronate)
4-ForMyl-3-Methylphenylboronic acid
Propionic acid
Erbium(III) chloride hydrate (99.999+%-Er) (low Ca, Fe, Mg) PURATREM
4-Fluoro-N-isopropylbenzamide
5-Carbamoyl-2-fluorobenzeneboronic acid 95%
(3-bromophenoxy)acetic acid(SALTDATA: FREE)
6-Bromoindole-2-carboxylic acid
4,6-Dichloro-2-(methylsulfonyl)pyrimidine
Phenthoate
Methyl indole-4-carboxylate
6-Piperidinopyridine-2-carboxylic acid
4,4''-DiaMino-p-terphenyl
Ethyl 6-broMo-7-MethyliMidazo[1,2-a]pyridine-2-carboxylate
TriethylenetetraMine, 60%
PotassiuM Pyrosulfate, Reagent, ACS
Hexyl 4-Hydroxybenzoate
2-Iodobenzoyl chloride
2,3-Dimethoxybenzyl alcohol
3-phenylpyrrolidine
Acetyl-beta-methylcholine bromide
6-Chlorouracil
N-[(tert-Butoxy)carbonyl]-L-tryptophan
Ethyl 4-AMinobutyrate Hydrochloride
4-IsopropylphenylMagnesiuM broMide, 0.5M in 2-MeTHF
CinnaMylideneacetophenone, 98+%
3,5-Difluorophenylacetic acid
N-Ethyl-N-(3-sulfopropyl)aniline, sodiuM salt
TETRAVINYLTIN
PotassiuM chroMate
L-(-)-MANNOSE
Poly(1-vinylpyrrolidone-co-vinyl acetate)
4'-Ethoxyacetophenone
2,6,8-Trimethyl-4-nonanol (threo- and erythro- mixture)
4-methyl-2-pyrrolidinone(SALTDATA: FREE)
(3-pyridin-4-ylpropyl)amine(SALTDATA: FREE)
L-Valine benzyl ester hydrochloride
1,4-DIPHENYLBUTADIYNE
Methyl 1-methyl-2-pyrroleacetate
2-(Dicyclohexylphosphino)-1-phenylindole
Zinc chloride
H-D-Phe-OtBu.HCI
Thujone (alpha- and beta- Mixture)
2,3,5,6-TetraMethyl-p-phenylenediaMine, 99%
3,5-DiMethoxy-4-hydroxybenzaldehyde, 98%
3-Pyridinecarboxaldehyde
beta-IsatoxiMe [for Metal Analysis]
1-methyl-1-propenylmagnesium bromide
propan-2-yl pyridin-3-ylcarbamate
3-(4-fluorophenyl)-1-phenyl-1H-pyrazole-4-carbaldehyde
thionicotinamide adenine dinucleotide phosphate monos. sa
1-Ethylpyridinium bromide
4-Chloro-3-(trifluoroMethylthio)benzyl broMide, 97%
Bis(N,N-diMethylacetaMide) Hydrogen DibroMobroMate , 97%
7-Methoxy-1-tetralone
Chloromethyl Thiocyanate
Benzyl trimethylammonium bromide
4-Iodo-4',4''-dimethoxytriphenylamine
3-Fluoro-4-(trifluoromethoxy)phenol
2-Nitrophenylpyruvic acid
2-(4-Amino-2-nitroanilino)-ethanol
2,6-Dichloro-4-(trifluoromethyl)pyridine
(R)-4-Boc-2-methyl
4-AMino-3-nitrobenzonitrile
1,6-Bis(acryloyloxy)-2,2,3,3,4,4,5,5-octafluorohexane
Ethyl 3-phenylpyrazole-5-carboxylate
CadMiuM sulfate, 8/3-hydrate, ACS reagent, crystals
2-AMino-6-chlorobenzonitrile, 97%
3-(TrifluoroMethyl)benzenesulfonyl Chloride
Bis(2-ethylhexyl) Sebacate
3-nitrobenzoic acid sodium salt
4-chloro-3-iodoquinoline
3-fluoro-D-phenylalanine
4-(Difluoromethoxy)benzaldehyde
CK 20 ELISA Kit
αEP ELISA Kit
Human MUC20 ELISA Kit
Human Cpn-Ab ELISA Kit
Chicken BMP-4 (Bone Morphogenetic Protein 4) ELISA Kit
S100β ELISA Kit
IL-2 ELISA Kit
Human growth hormone releasing hormone,GHRH ELISA Kit
Human VEGFB ELISA Kit
STAT4 ELISA Kit
Fastidious Anaerobe Agar (FAA)
Yeast Synthetic Drop-out Agar Medium without Histi
IsobutyraMide
TRILAURIN
5-Chlorobenzo[b]thiophene, 97%
2-Fluorobenzonitrile, 99%
2-Iodobenzoyl chloride
Ginsenoside Rg3
3-Ethoxypropionaldehyde diethyl acetal
3-Fluoro-2-nitrophenol
2-Amino-4-methylbenzonitrile
CERIUM(IV) SULFATE TETRAHYDRATE
3-Hydroxy-3-Phenyl-Propionic Acid
3-Hexadecanone, 95%
DL-Aspartic acid, 98+%
Bis(2-carboxyethylgerManiuM(IV) sesquioxide)
3-BroMo-5-fluorobenzotrifluoride
ISOPROPYLSULFONYL CHLORIDE
2-ACETAMIDO-5-BROMOBENZOIC ACID
6-AMinopyridine-2-carboxylic acid
4-ETHYLAMINO-1-BUTANOL
2-(Trifluoromethyl)phenyl isocyanate 97%
2-Chloro-4-morpholinobenzenecarboxylic acid
Sodium periodate, for analysis, 99%
1-Bromo-3-[2-(tert-butyldimethylsilyloxy)ethyl]benzene 98%