COLO201细胞系—人结直肠腺癌细胞系国外引进 货号:GD-C16718977
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞系—为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Diethyl oxalate, 99%
SalicylhydroxaMic acid
S-(2-Phenoxyethyl)thioacetate
PalladiuM hydroxide
D-ALPHA-TOCOPHERYLQUINONE
1H-Indazole-6-carboxaldehyde
Mono-Methyl terephthalate
3-Methacryloxypropyltris(methoxyethoxy)silane tec(3-Tris(2-methoxyethoxy)silyl propylmethacrylate
1-(4-Methoxy-pyridin-2-yl)-ethanone
1,2,3,4,6-Penta-O-acetyl-α-D-galactopyranose ,98%
Hexamethyleneammonium Hexamethylenedithiocarbamate
Bromoxynil
21-DEOXYCORTISONE
3,4-Difluorobenzotrifluoride
4-Piperidinobenzoic acid
4-Chlorophenylacetone
trans-N,N'-Diacetylcyclohexane-1,2-diaMine
2,4-Dichlorobenzyl broMide
trans-4-Phenyl-3-buten-2-one, 99%
PIPES DipotassiuM Salt, Biological Buffer
Ethyl Anthranilate
L-m-Tyrosine
4,7-dichloro-1,10-phenanthroline
D-Pantothenic Acid Sodium Salt
β-Phenylethylamine hydrochloride
PYRAZINE-N,N'-DIOXIDE
4-Fluorophenoxyacetic acid
2-Ethylbutyl acetate, 97%
3-Chloro-2,6-difluorobenzoic acid, 97%
D-GlutaMic acid, 99+%
Geranyl chloride
N-tert-Butyl-3-MethoxybenzaMide
(3S,5R,8aS)-(+)-Hexahydro-3-phenyl-5H-oxazolo[3,2-a]pyridine-5-carbonitrile
Pyridine sulfur trioxide
2-AcetaMido-4-fluorobenzoic acid
3-Chlorophenyl isocyanate
N-(3-Aminopropyl)-N-methylaniline
alpha,alpha-dibromo-4'-fluoroacetophenone
trans-3-Amino-1-Boc-4-methoxypyrrolidine
(3-Bromobenzyl)dimethylamine
Sodium 3,5,6-trichloropyridin-2-olate
3-METHYL-1-PHENYL-2-PHOSPHOLENE 1-OXIDE
S-BUTYRYLTHIOCHOLINE IODIDE
Granisetron Related CoMpound D;1-Methyl-1H-indazole-3-carboxylic acid
2-Methyl-2-[(7-Methyl-2,3-dihydro-1H-inden-4-yl)oxy]propanoic acid
Sulfonazo III [SpectrophotoMetric reagent for alkaline earth Metals and indicator for the precipitation titration of SO4 with Ba]
5-Hydroxy-3,4-dihydro-2(1h)-quinolinone
4-Methylsulphonylphenol, 95%
3-Phenoxybenzoyl chloride, 97%
2-Amino-4,6-dimethylpyrimidine
Acetoxy-2-propanone (stabilized with Mg6Al2(OH)16CO3.4H2O)
Cyproterone acetate
methyl-2,3-dideoxy-3-fluoro-5-o-(4-phenylbenzoyl)-alpha-d-erythro-pentofuranoside
Valproate semisodium
4-Isopropylbenzenesulphonyl chloride
2-BENZYLIDENECYCLOHEXANONE
SodiuM Ligninsulfonate
2-Fluoro-4-(trifluoroMethyl)pyridine
Phthaloyl dichloride
2,2,3,3,4,4,4-Heptafluoro-1-butanol, 95%
4-Benzyl-3-hydroxymethylmorpholine
Hydroxypropyl methylcellulose phthalate 55
Potassium (E)-propenyl-1-trifluoroborate
4-(TRIMETHYLSILOXY)BENZALDEHYDE
N6-Cbz-L-Lysine benzyl ester hydrochloride
2,6-Dichloroquinoxaline
2,5-DiMethylthiophene;2,5-Thioxene; α,α′-Thioxene;
AMaranth
1,4-Bis(3-hydroxyphenoxy)benzene
2-(t-ButylaMino)sulfonylphenylboronic acid
alpha-Methyl-3-(trifluoroMethyl)benzyl alcohol, 96%
N1-(5-AMino-2-Methylphenyl)acetaMide, 97%
6-Phenyl-2-pyridone
Ethylgonendione
N,N-dibutylaniline
1H-Indole-1-carboxylicacid, 5-bromo-2,3-dihydro-, 1,1-dimethylethyl ester
5-bromo-D-tryptophan
(Z)-2'-(1H-imidazol-1-yl)-2,4-dichloroacetophenone oxime
CKMT1A ELISA Kit
Mouse β-Defensins,β-DF ELISA Kit
Human ITGA5 ELISA Kit
sPLA2 ELISA Kit
AmAC-1 ELISA Kit
Rat MAPK (P38 Mitogen-Activated Protein Kinase) ELISA Kit
IFN-α ELISA Kit
Human PLXND1 ELISA Kit
Human AGTR2 ELISA Kit
FLNa ELISA Kit
Yeast Synthetic Drop-out Agar Medium
Wallerstein Laboratory Nutrient Agar
Isododecane, Mixture of isoMers, tech. 80%
Iron(II) perchlorate hydrate, Reagent Grade
Ethyl 5-Chloro-2-Indolecarboxylate
SODIUM GLYCEROPHOSPHATE HYDRATE
L-m-Tyrosine
4-acetoxy-3-methoxybenzoic acid
Silver bromide
3-fluoro-4-methoxybenzonitrile
2-BROMO-5-NITROBENZOIC ACID
4-Isopropylbenzeneboronic Acid
p-Benzoquinone dioxiMe, 95%
1-Acetylpiperidine-4-carboxylic acid, 98+%
StrontiuM, distilled dendritic pieces, 99.8% (Metals basis)
HexadecylpyridiniuM broMide
Butyl butyrate
(R)-(?)-HexahydroMandelic acid
4-Cyanophenyl isocyanate
Ethylboronic acid
3,4-Difluorophenylglyoxal hydrate, 98%, dry wt. basis
2,6-Difluoro-4-methoxybenzonitrile
Zinc stannate
Ethyl 1-(2,4-difluorophenyl)-7-Cl-6-F-4-O-hydropyridino[2,3-b]pyridine-3-carboxylate, 99%
3-(Bromomethyl)-1,2-benzisoxazole