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RH30[RC13,RMS13,SJRH30]细胞系—人横纹肌肉瘤细胞系来电咨询更多惊喜

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  • RH30[RC13,RMS13,SJRH30]细胞系—人横纹肌肉瘤细胞系来电咨询更多惊喜 货号:GD-C16618253

    细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
    产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
    公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
    产品规格:≥1*10(6)/ml
    安全级别:1
    物种来源:人源或鼠源等
    细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
    细胞冻存的步骤:1)选择处于对数生长期的细胞系—在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉,用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶,加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞系—使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min,10分钟);2)去上清液,加入含20%小牛血清的完全培养基,于4℃预冷15分钟后,逐滴加入已无菌的DMSO或甘油,用吸管轻轻吹打使细胞均匀,细胞浓度为5×106~1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢?答案是不一定的,具体要看培养的细胞类型来选择;3)将分装上述细胞悬液于2ml冻存管中,每管0.25ml。冻存管要将盖子盖紧,并标记好细胞名称和冻存日期,同时作好登记(日期、细胞种类及代次、冻存支数);4)将装好细胞的冻存管放到冻存盒中,-80℃冰箱过夜。;如果有程序降温器(放在-80℃冰箱过夜,放入液氮罐)Z好;或者可以在4℃,2h;然后转到-20℃,2h;-80℃,2h;放入液氮罐;5)细胞冻存在液氮中可以长期保存,但为妥善起见,冻存半年后,Z好取出一只冻存管细胞复苏培养,观察生长情况,然后再继续冻存。
    细胞特性:贴壁/悬浮等
    细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
    培养条件:详见细胞说明书
    传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
    tert-Butyl (S)-1-Boc-5-oxopyrrolidine-2-carboxylate, 95%
    2',4'-DiMethylacetophenone
    2-(4-AMinophenyl)ethanol
    TetrabutylaMMoniuM fluoride
    MethyltriphenylphosphoniuM broMide
    1,3,5,7-TetraMethyl-6-phenyl-2,4,8-trioxa-6-phosphaadaMantane
    4-NITROPHENYLGLYOXYLIC ACID
    1-(Carbamoylmethyl)pyridinium Chloride
    9-Decenoic acid
    (1-methyl-1H-benzimidazol-2-yl)methanol(SALTDATA: FREE)
    5-Bromo-2-nitrophenol 98%
    2,6-Dichlorobenzothiazole
    ZINC CHROMATE
    Praseodymium(III) nitrate hexahydrate
    Methyl 1-(Mercaptomethyl)cyclopropaneacetate
    Diethyl phosphonoacetaldehyde diethyl acetal
    4,4'-Dipyridyl Sulfide
    4-BroMoisoquinolin-1-ol
    hexahydrate, 98%
    EthylenediaMinetetraacetic Acid MagnesiuM DisodiuM Salt
    1-(tert-Butoxycarbonyl)-2-piperidone
    2-Ethoxybenzoic acid
    m-chloroanisole
    Sodium carbonate
    Formic acid
    1-IODO-2,3,5-TRICHLOROBENZENE
    5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt
    4-Fluoro-N-(2-fluorophenyl)benzaMide, 97%
    ZirconiuM(IV) n-propoxide, 70% w/w in n-propanol, packaged under Argon in resealable CheMSeal^t bottles
    Pentafluoropyridine, 99%
    AMMoniuM hypophosphite
    Dibutyltin Maleate
    BORON TRIFLUORIDE, N-PROPANOL REAGENT 15
    γ-Phenyl-α-(trifluoroMethyl)-γ-butyrolactone,Mixture of cis and trans
    2,6-DiMethoxybenzoyl chloride
    EthylenediaMinetetraacetic acid dipotassiuM salt dihydrate
    2-Ethoxycarbonylbenzeneboronic acid
    4-(2-Bromoacetyl)benzonitrile
    Phenylmalonic Acid
    Azelanitrile
    2-Mercapto-3-butanol
    2-CHLORO-4-NITROBENZOYL CHLORIDE
    BIS(2,2,2-TRIFLUOROETHYL) ETHER
    TaMoxifen;(Z)-2-[4-(1, 2-Diphenyl-1-butenyl)-phenoxy] -N, N- diMethylethanaMine;1-p-β-DiMethylaMinoethoxyphenyl-trans-1,2-diphenylbut-1-ene
    3-Chloro-2-Methylphenyl isothiocyanate
    3-BenzalbutyraMide
    N-Methoxy-N-MethylbutanaMide
    6-Chlorooxindole, 98%
    2-Chloro-6-Methoxy-3-nitropyridine, 97%
    4-tert-Butylcyclohexanol (cis- and trans- Mixture)
    2-Chloro-1-MethylpyridiniuM p-Toluenesulfonate
    Lithium benzoate
    4-nitrophenyl-β-D-galactopyranoside
    Rose Hip Oil
    N,N,N',N'-Tetraethyl-1,3-propanediamine
    2,3,5-Ttrifluorobenzaldehyde
    4-(4-DiMethylaMinophenylazo)phenylarsonic Acid Hydrochloride , 95%
    Benzenesulfonic acid
    Crotononitrile (pract)
    Itaconic Anhydride
    D-Glucose ,98%
    DL-3-Methyl-2-butanol, 98%
    2'-methoxybiphenyl-3-carboxylic acid(SALTDATA: FREE)
    Zileuton
    5-CHLORO-2-METHOXYANILINE HYDROCHLORIDE
    3-BROMO-4-(TRIFLUOROMETHOXY)ANILINE
    Kryptofix 222
    cis-2-(tert-ButoxycarbonylaMino)cyclohexanecarboxylic acid
    (-)-(R)-N,N-DiMethyl-1-[(S)-1′,2-bis(diphenylphosphino
    2-TriMethylsilyl-1,3-dithiane
    2,9-DiMethyl-4,7-diphenyl-1,10-phenanthroline, 98%
    PotassiuM tetracyanoplatinate(II) trihydrate
    FLUORESCEIN DIACETATE
    DAPT
    D-Luciferin Sodium Salt
    Boc-N-methyl-D-leucine
    2-Amino-1,3-thiazole-5-carboxylic Acid
    8-Methoxyquinolin-2(1H)-one
    TFPI ELISA Kit
    Mouse FOXP3 (Forkhead Box Protein P3) ELISA Kit
    tPA ELISA Kit
    BGP ELISA Kit
    PⅢNP ELISA Kit
    Apramycin ELISA Kit
    AT-1 ELISA Kit
    Human CSRP1 ELISA Kit
    Human Surface Membrane Immunoglobulin,SmIg ELISA Kit
    Anti-AChR ELISA Kit
    Fluid Soybean-Casein Digest Broth
    Sterile penicillin solution
    Zoledronic acid hydrate
    SulfaMethoxazole
    5-BroMo-2-pyridineacetic acid, 98%
    2-Cyano-3-iodopyridine, 95%
    2-Ethoxybenzoic acid
    methyl heineicosanoate
    2-Ethylbutyl methacrylate
    2-Chlorobutane

    Gibberellic acid
    ACID RED 66
    N-alpha-(tert-Butoxycarbonyl)-L-lysine
    3,5-Bis(Methoxycarbonyl)benzeneboronic acid, 97%
    Acetylcholine chloride, 98+%
    CarboxyMethyl cellulose
    Erlose
    2-CYCLOHEXEN-1-ONE DIMETHYLKETAL
    4-(HydroxyMethyl)phenylboronic acid
    2,6-DICHLOROBENZYLIDENEACETONE
    N,N'-DI-TERT-BUTYLETHYLENEDIAMINE
    4-aminotetrahydrothiophene-3-ol 1,1-dioxide(SALTDATA: FREE)
    (-)-Dibenzoyl-L-tartaric acid monohydrate, 98%
    4-Piperazin-1-ylbenzaldehyde
    1-Aminoanthracene
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