L6565细胞系—小鼠白血病克隆细胞系要多久复苏 货号：GD-C16419108
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
(^+)-Tetrahydrofurfuryl alcohol, 98%
Angelicin
3-Fluoro-4-(trifluoroMethoxy)benzyl broMide, 97%
4-CYANOPHENYL 4-HEPTYLBENZOATE
TERT-BUTYLCYCLOHEXANE
3-CHLOROANILINE HYDROCHLORIDE
4-TERT-BUTYLBENZOPHENONE
3-(1H-Pyrrol-1-ylmethyl)pyridine, 97%
Dimethyl (2-Oxopropyl)phosphonate
3-(3-aminophenyl)-1,3-oxazolidin-2-one(SALTDATA: FREE)
Ethyl thiophene-2-glyoxylate
2-Amino-3-hydroxy-2'-(2,3,4-trihydroxybenzyl)propionohydrazide
N-Boc-1,4-cyclohexanediamine
4-CYANO-4-PHENYLCYCLOHEXANONE
4-BroMo-1H-pyrazole-3-carbonitrile
Succinic Acid
DiMethyl Tetrafluorosuccinate
3-Undecanol
Triphenylacetic acid, 99%
Riboflavin, USP
SodiuM 2,3,5,6-Tetrafluoro-4-hydroxybenzenesulfonate
Benzyl carbamate
2-Hydroxy-4,5-Dimethoxy Benzoic Acid
4-Bromomethylcinnamic acid methyl ester
4H-cyclopenta[1,2-b:5,4-b']bisthiophene
4-(Dimethylamino)phenylboronic acid
TETRADODECYLAMMONIUM BROMIDE
3-(3,4-DihydroxycinnaMoyl)Quinic Acid
ErbiuM(III) isopropoxide
2,3,5,6-Tetrafluorobenzyl broMide, 98+%
Diethylene glycol Monoethyl ether
5-Norbornene-2-carbonitrile, Mixture of isoMers
2-PROPYL-1-HEPTANOL
PhosphoMolybdic acid solution
Cedar leaf oil
N-TETRADECYLPHOSPHONIC ACID
4'-Pentylacetophenone
Methoxy(4-methoxyphenyl)dimethylsilane, 97%
L(-)-alpha-Bromocamphor-pi-sulfonic acid, ammonium salt, 99%
4-Triphenylmethylphenol
4-Iodopyrazole
1,4-BIS(CHLOROMETHYL)TETRAFLUOROBENZENE
5-CARBOXYOXINDOLE
Taurocholic acid sodiuM salt ;
2-Quinoxalinecarboxylic acid
CalciuM phosphorylcholine chloride
Bis(4-nitrobenzyl) Malonate
Didecyl diMethyl aMMoniuM chloride, 80% aqueous solution
6-Phenoxy-3-pyridinesulfonyl chloride, 95%
4'-Bromoacetophenone
5-Methyl L-GlutaMate
Crotonyl chloride
6-Fluoro-4-hydroxy-2-(trifluoromethyl)quinoline
2-[2-(DIPHENYLPHOSPHINO)ETHYL]PYRIDINE
Octadecylbenzene
2-Formylphenoxyacetic acid
NeodyMiuM trifluoride
1,1'-BIS(DICYCLOHEXYLPHOSPHINO)FERROCENE
2-AMino-7-broMofluorene
Terephthalaldehyde Bis(diethyl Acetal)
4-Iodobenzoic acid, 98%
3-Amino-2-bromo-6-methoxypyridine ,97%
1-Acetyl-3-indolecarboxaldehyde, 98%
Glycol sulfite
2,3,6-TRIFLUOROBENZYL ALCOHOL
o-Tolunitrile
2-Nitropropane;β-Nitropropane
2-Chlorobenzonitrile
1-Acetyl-2-iMidazolidinone
2-(4-TrifluoroMethylphenyl)pyrrolidine
4-BroMobutyryl chloride, 95%
1-(4-AMino-2-hydroxyphenyl)ethan-1-one, Tech.
L-Tyrosine hydrochloride
1-Acetyl-4-piperidone
3,7-dimethyloct-7-en-1-ol
N-Boc-D/L-Alaninol
3,5-Dinitrobenzyl alcohol
anti-3-oxotricyclo(2.2.1.0(2,6))heptane-7-carboxy
VB6 ELISA Kit
Mouse α1-Acid glycoprotein,α1-AGP ELISA Kit
Human REG1β ELISA Kit
Human non-neuronal enolase,NNE ELISA Kit
SOD ELISA Kit
Rat Toll-like receptor 4,TLR4 ELISA Kit
Chicken Laminin,LN ELISA Kit
Human CCSA-2 ELISA Kit
Human anti-Endostatin antibody,ES-Ab ELISA Kit
VCAM-1 ELISA Kit
Linsmaier & Skoog Basal Medium
Fastidious Anaerobe Broth (FAB)
(-)-DIOP, 98%
Benzyl glycinate hydrochloride
5-Chloro-2-fluoropyridine, 97%
2-Fluoro-6-nitrobenzyl broMide, 98+%
Benzyl carbamate
Vat Red F3B
Immunostimulants,adjuvants, Freund's
2,5-bis(trifluoromethyl)benzaldehyde
D-3-Bromocamphor-8-sulfonic acid ammonium salt
2,2'-OXYBIS(ETHYLAMINE) DIHYDROCHLORIDE
DiMeric Mercapto Propanone
N^a-Boc-N^e-FMoc-D-lysine, 95%
4-Fluoro-1-iodo-2-nitrobenzene, 97%
4,4'-DibroMobiphenyl, 98+%
3-BroMo-4,5-difluoroaniline
3,6-DibroMo-9-ethylcarbazole
TRIDECANOL
(3-BROMOPROPYL)PHOSPHONIC ACID
1,7-DIMETHYLNAPHTHALENE
MALEIC ACID MONO(2-ETHYLHEXYL) ESTER
3-Iodobenzaldehyde
Dibenzylamine 98%
p-Toluoyl chloride ,98%
