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C-33 A细胞系—人子宫颈癌细胞系要多久复苏

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  • C-33 A细胞系—人子宫颈癌细胞系要多久复苏 货号:GD-C16419102

    细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
    产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
    公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
    产品规格:≥1*10(6)/ml
    安全级别:1
    物种来源:人源或鼠源等
    细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
    购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
    细胞特性:贴壁/悬浮等
    细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
    培养条件:详见细胞说明书
    传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
    Triethylborane, 1M soln. in THF
    5-Methyl-2-hexanone
    Tetrakis(acetonitrile)copper(I) tetrafluoroborate
    2-BroMo-4,6-difluorophenol, 98+%
    ETHYL N-ETHYLCARBAMATE
    Maltitol
    (-)-PERILLALDEHYDE
    1-Tetrahydro-2H-pyran-3-ylmethanamine
    6-Chloronicotinonitrile
    2-(Hydroxymethyl)-15-crown 5-Ether
    3-Bromobenzylamine, 95%
    3-Hydroxy-4-nitrobenzaldehyde
    Isopropylphenyl phosphate
    STEARYL ACETATE
    2-BroMobenzyl BroMide
    Indole-5-carboxylic acid
    1,4-DibroMooctafluorobutane
    Methyl 2-benzyloxy-4-broMobenzoate
    Tri-n-propylsilane, 99%
    Resveratrol
    4-(TrifluoroMethyl)benzylaMine
    tricaprin
    Rhodanine-N-acetic acid
    1H-pyrrole-2,3,5-tricarboxylic acid
    7-Fluoroindole
    4-Bromo-3-fluorobenzenesulfonamide
    2,3-Dichlorobenzaldehyde

    3'-Chloro-4'-fluoroacetophenone, 97%
    SeleniuM dichloride oxide, 99% (Metals basis)

    2-Fluorobenzonitrile
    2-Furanylboronic acid MIDA ester
    Phthalic anhydride
    Bis(diphenylphosphino)Methane
    1-PHENYL-1-NONYNE
    2,5-Difluorobenzotrifluoride
    2,6-Dibromo-4-isopropylaniline
    3-(4,4,5,5-Tetramethyl-1,3,2-dioxaborolan-2-yl)acetanilide ,98%
    4-Methoxysalicylamide
    L-Glutamic acid 5-methyl ester
    METHYL 4-CHLORO-2-NITROBENZOATE
    Cefpirome sulfate
    DL-3-Methylvaleric acid
    1-Chloro-2-iodobenzene (stabilized with copper chip)
    Cholesterol Lauryl Carbonate
    2-(MethylcarbaMoyl)phenylboronic acid
    EthylenediaMinetetraacetic acid, disodiuM salt, 99+%, for Molecular biology, DNAse, RNAse and Protease free
    2-Chloro-6-fluorophenylboronic acid, 97%
    3-BroMo-o-xylene
    Ethyl 3-AMinobenzoate
    2-Aminopyrimidine-4-carboxylic acid
    2-Methoxybiphenyl
    (1S,3R)-N-BOC-1-Aminocyclopentane-3-carboxylic acid
    1,1'-Biphenyl, 4-bromo-4'-iodo-
    Amino tris(methylene phosphonic acid)
    3-Chloro-2-fluorothioanisole
    4-Benzylaniline, HCl
    RuCl2[(S)-(DM-BINAP)][(S)-DAIPEN]
    1-(2-Cyanoethyl)-3-(trifluoromethyl)-pyrid-2-(1H)-one
    3,4-Dimethylaniline, 99.5%
    Allylacetoacetate, 98%
    4-Aminothiomorpholine 1,1-Dioxide
    Methyl-4-nitro-1-methyl pyrrole-2-carboxylate
    2-Bromo-1-[4-(methylsulfonyl)phenyl]-1-ethanone
    Tetramethoxymethane
    2,2-Dimethylpentane
    SodiuM hyaluronate
    1-AcetaMidoadaMantane
    BisMuth SodiuM EthylenediaMinetetraacetate
    2-(BenzylaMino)nicotinonitrile
    1-BroMooctadecane, 96%
    2,4,5-TRICHLOROANISOLE
    Bis(4-chlorobutyl) Ether
    3-Methyl-2-pyridinol
    Vanadium atomic absorption standard solution
    2,7-Naphthalenedisulfonic acid disodium salt
    methyl O-acetylsalicylate
    VD3 ELISA Kit
    Mouse α1-microglobulin,α1-MG ELISA Kit
    Human PSEN1 ELISA Kit
    Human LTA ELISA Kit
    IC ELISA Kit
    TLL1 ELISA Kit
    Chicken C3 (Complement Component 3) ELISA Kit
    Human FMR1 ELISA Kit
    Human VCAM-1 ELISA Kit
    VEGFR 3 ELISA Kit
    N6 Medium without Agar
    Glfu Anaerobic Medium Agar
    AluMinuM cobalt isopropoxide, 99.9% (Metals basis), 10% w/v in isopropanol
    Glycine Methyl ester hydrochloride, 99%
    5-Chloro-2-Methoxypyridine, 98%
    2-FluorobenzylaMine, 97%
    tricaprin
    Reactive Yellow 39
    calcitonin
    5-fluoroquinoline

    DL-2-Amino-1-butanol
    Cupric Sulfate
    2-Benzyloxypyridine-3-boronic acid pinacol ester, 95%
    3',4'-Dichloroacetophenone, 98+%
    Ethyl (phenylthio)acetate, 97%
    HolMiuM chloride hexahydrate
    N-HydroxyMaleiMide
    (R,R)-(+)-N,N′-Bis(α-Methylbenzyl)sulfaMide
    FUMARAMIDE
    BenzyldiMethylhexadecylaMMoniuM chloride
    Ethyl 3-Methylthiopropionate
    Perfluoroglutaryl chloride 98%
    3,5-Dichloro-4-hydroxybenzaldehyde
    Octadecyl Isocyanate
    Cobalt(II) carbonate hydrate (99%-Co) (Co min. 45.5%)
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