KNS-42细胞系—人脑胶质瘤细胞系要多久复苏 货号：GD-C16420168
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题：如不能在收到细胞后及时操作细胞系—T25及离心管发货的细胞将可以消毒后在37度过夜，血清发货的细胞在室温下静置1-2天(注意不要放冰箱)，干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱，若干冰完全升华，请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境，同时使部分因运输导致脱落的细胞贴壁，此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞系—可以收集上清后离心(1200rpm，5min)后，将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中，由于不断震荡及环境不适，可能导致细胞破碎死亡，从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下，平衡后，贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可；悬浮细胞可以离心(1000rpm，3min)收集细胞系—并用PBS重悬后再离心一次，再用新的培养基重悬并接种细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-Methylpentane, 99+%
2,6-Dinitrochlorobenzene
DIMETHYL CIS-1,2,3,6-TETRAHYDROPHTHALATE
((2,4,6-Tri-isopropyl)phenyl)di-cyclohexylphosphine
LevetiracetaM
(R)-(+)-1-Phenylethyl isocyanate
2-Chloro-4-trifluoroMethoxyphenylboronic acid
tert-Butyl 5-bromo-2-chlorobenzoate
2-(p-Tolyl)ethylamine ,97%
3-Chloro-4-fluoroanisole
2-Chlorophenyl Cyclopentyl Ketone
Cbz-Cyclohexyl-L-glycine
TRANS-2-AMINOCYCLOHEXANOL HYDROCHLORIDE
1-[[2,4,6-Tris(isopropyl)phenyl]sulphonyl]-1H-1,2,4-triazole
4-(Benzyloxy)-2-fluorophenylboronic acid
Kojic acid
1,12-Diacetoxydodecane
N,N-DiMethyl 2-broMo-5-fluorobenzaMide
Tributyl phosphate, 99+%
Polysorbate 80, NF
2,3,4,5-Tetrafluorobenzoyl Chloride
isopropyl salicylate
2,5-Dichlorobenzenesulfonyl chloride
1,7-Octadiyne
3-Hydroxy-5-(trifluoromethyl)benzoic acid
2-Acetamido-2-deoxy-D-glucose
Isopropyl acetoacetate
Alpha,Alpha-Diphenyl-L-Prolinol
3-(4-BroMophenyl)propionic acid, 98%
L-Serine Methyl ester hydrochloride, 98%
2,2'-Dithiobis(4-Methylthiazole)
Butyl ether
ACRIFLAVINE
PROPIONALDEHYDE DIETHYL ACETAL
1,1-Difluoroethane
4-Ethyl-o-xylene , 99%
Triphenylantimony, 97%
4-Bromoisatin 98%
Ethyl 3-amino-1H-indole-2-carboxylate
Aminoguanidine hydrochloride, 98%
Myristic acid
3-(1H-Pyrrol-1-yl)aniline
2-CHLORO-2-HYDROXYIMINOACETIC ACID ETHYL ESTER
Acenaphthene;1,8-Ethylenenaphthalene;1,2-Dihydroacenaphthylene
3-Chloro-4-fluorobenzoyl chloride
Fast Violet B salt
Nonanenitrile
4-Iodo-1-isopropyl-1H-pyrazole
5-AMino-1H-benziMidazole-2-thiol, 97%
GLY-D-ALA
2-Methoxy-5-Methylphenol
Ethyl 1-methylpipecolinate
Empenthrin
glycidyl propargyl ether
Amberlyst
COBALT CHROMATE
2-(3-BroMophenyl)-5-propyl-1,3,4-oxadiazole
D-Ribose
3-BroMo-6-chloroiMidazo[1,2-a]pyridine
Piperine
5-bromo-4,6-dimethyl-1H-pyrazolo[3,4-b]pyridin-3-amine(SALTDATA: FREE)
4-(4-Bromophenyl)thiophene-2-carbonyl chloride
(1R,3R)-N-BOC-1-Aminocyclopentane-3-carboxylic acid, 98% ee, 95%
Ethyl 4-methylcinnamate
Molybdenum trioxide
4-Nitro-4'-aminostilbene-2,2'-disulfonic acid
1(2H)-Phthalazinone
Copper(II)-ethylenediaMine coMplex
1,4-CyclohexanediMethanol Bis(2-ethylhexanoate) (cis- and trans- Mixture)
2-Methylphenylboronic acid, pinacol ester
Fluorescein, pure
2-BroMoindene, 99%
1,4-DibroMopentane
TriMethylphenylaMMoniuM Hydroxide (20-25% in Methanol) [for On-ColuMn Methylation]
(S)-(+)-α-amino-γ-butyrolactone hydrochloride
2-Amino-5-naphthol-7-sulfonic Acid
2-Oxazolidinone, 5-(chloromethyl)-, (5S)-
N-Boc-D-Phenylalaninol
PAI ELISA Kit
Mouse GLb (Galactosidase, Beta) ELISA Kit
Human CENPA (Centromere Protein A) ELISA Kit
Human SNX3 ELISA Kit
Chicken TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit
SP ELISA Kit
Chicken Interleukin 1,IL-1 ELISA Kit
Human growth hormone releasing hormone,GHRH ELISA Kit
Human ANG II R2/AGTR2 (Angiotensin II Receptor 2) ELISA Kit
CKMT1B ELISA Kit
Sulfite Iron Agar
Modified Melin-Norkrans Agar
Isoquinoline-3-carbonitrile, 99%
PotassiuM perchlorate, 99%
5-Chlorothiophene-2-carbonyl chloride, 98%
2-Fluoropropionic acid, 96%
isopropyl salicylate
tetrakis(phenylmethyl)thioperoxydi(carbothioamide)
Ammonium chromate
nonadecafluorodecanoic acid
2-CHLORO-5-NITROPHENYL ISOCYANATE
1-PHENYL-1-CYCLOPROPANECARBONITRILE
Vanillic acid hydrazide, 98+%
4-MethylsulfaMoylbenzeneboronic acid, 97%
4,4-DiMethyl-3-thioseMicarbazide, 95%
2-Chloro-4-fluoropyridine
BIS(TETRA-N-BUTYLAMMONIUM) BIS(MALEONITRILEDITHIOLATO) NICKEL COMPLEX
2-Cyano-6-Methoxynaphthalene
N-Butyl 3-broMo-5-MethylbenzenesulfonaMide
RhodiuM(III) nitrate
3-Methyl-1-pentyn-3-ol
3-(4-Methoxyphenyl)-1H-pyrazole-4-carbaldehyde, 95%
(1S,5R)-1,3,3-trimethyl-6-azabicyclo[3.2.1]octane(SALTDATA: FREE)
Thiophene-2-sulfonamide
[1-(3-Chloro-phenyl)-3-pyrrolidin-1-yl-propyl]-methyl-amine
