A-673细胞系—人横纹肌肉瘤细胞系哪家专业 货号:GD-C16119144
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
(^+)-2,2'-Bis(diphenylphosphino)-1,1'-binaphthyl, 98%
EthylenediaMinetetraacetic acid disodiuM zinc salt tetrahydrate
N,N-DiMethylheptylaMine N-oxide hydrate
1-(3-TrifluoroMethylphenyl) hydrochloride
2-Propanol-1,1,1,3,3,3-d6
ETHYL ORANGE
1,1-DICHLOROPINACOLIN
o-Nitroacetophenone, 95%
2,6-Dimethylphenyl isocyanate
2-Nitro-5-(trifluoromethyl)aniline ,98%
N-(2-chlorobenzyl)butan-1-amine(SALTDATA: HCl)
6-NITROPIPERONAL
1-Diethylamino-2-propyne
1-IODO-1-(TRIFLUOROMETHOXY)TETRAFLUOROETHANE
1,3-Diacetylbenzene
1,1,1-Trifluoroacetone
1,3-Diiodo-5,5-diMethylhydantoin
1-Vinylnaphthalene (stabilized with TBC)
Tris(dioxa-3,6-heptyl)aMine, 95%
SiMethicone, USP
2,2-DiMethylvaleronitrile
4-Aminobenzyl alcohol
Diphenic anhydride
2-Cyanoethyltrichlorosilane
5'-Fluoro-2'-hydroxyacetophenone
COBALT(II) SELENIDE
2,3-Dichloropyridine
(R)-(-)-1-AMinoindane, ChiPros 99+%, ee 98+%
2,2'-Dinitrobiphenyl, 97%
O-tert-Butyl-L-threonine Methyl ester hydrochloride, 95%
5′-O-TritylthyMidine
N-FLUOROPYRIDINIUM PYRIDINE HEPTAFLUORODIBORATE
1-IsopropyliMidazole
Phenyl Methanesulfonate
Black pepper oil
4,6-Dihydroxy-2-MercaptopyriMidine
2,4,5-Trichlorobenzenesulphonyl chloride
Benzyltriethylammonium Chloride
4-Bromo-2,3-dimethylaniline
4'-Chloro-5-fluoro-2-hydroxybenzophenone 97%
3-Bromo-1-propanol
3-AMINO-4-HYDROXYBENZOIC ACID
Ferrozine disodium salt
Sodium 2-propylpentanoate
4-BroMo-2-chlorobenzoic acid
1-Benzyl-3-(ethylaMino)pyrrolidine
Ethyl 2-hydroxy-5-nitronicotinate
AMberlite? IRA-410, Cl-forM, ion-exchange resin
Tri-M-tolylphosphine, 98%
4-Hydroxypiperidine
3-(DicyanoMethylidene)indan-1-one
2'-Bromoacetanilide
1-Boc-3-hydroxymethyl
Benzene, 1-bromo-5-chloro-2-fluoro-4-methyl-
2-Bromo-6-fluoroaniline
4-Bromomethyl-3-nitrobenzoic acid
4-BENZYLOXYBENZOIC ACID
Octadecanophenone , 95%
4-Chloro-2-nitroanisole, 99+%
1-(5-Methyl-3-phenyl-4-isoxazolyl)-1-ethanone
Sodium Sulfamate
(1S,2S,4S,5S)-2,5-Bis(3,5-di-tert-butyl-2-hydroxybenzylideneamino)bicyclo[2.2.1]heptane
(S)-?-(m-Tolyl)alanine
3-Aminobenzenesulfonanilide
7-Methoxy-1H-indole
3-Fluoro-5-hydroxypyridine
Tetrabutyl orthosilicate
4-Fluorobenzaldehyde
1-AMinohoMopiperidine
3-BroMo-2-piperazinopyridine
3-Methoxy-2-nitrobenzaldehyde, 97%
4-(TrifluoroMethylthio)aniline, 95%
BARIUM OXIDE
3-Sulfolene
1,4-Benzoquinone
TRITICONAZOLE
2-(4-Methoxy phenyl)-6-methoxy benzo[b]thiophene
5-Phenylthiazol-2-amine
SYNPO ELISA Kit
Mouse FOS (V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog) ELISA Kit
Human Apolipoprotein C3,Apo C3 ELISA Kit
Human DEFβ115 ELISA Kit
Chicken CD40L/TNFSF5 (Cluster of Differentiation 40 Ligand) ELISA Kit
RELA ELISA Kit
Hen egg phosvitin phosphopeptides,PPP ELISA Kit
Human monoamine oxidase,MAO ELISA Kit
ANGPTL3 ELISA Kit
TPP ELISA Kit
Fluid Soybean-Casein Digest Broth
Medium 1(Penassay Seed Agar)
YTTRIUM(III) ISOPROPOXIDE
SodiuM hydrogen fuMarate, 98%
5-Chloro-1-Indanone
2-Fluoro-5-Nitrophenylboronic Acid Pinacol Ester
4-Aminobenzyl alcohol
2,4-dichloro-6-methyl-5-nitropyrimidine
Poly(dibutyltitanate)
α,α,α-trifluoro-4-iodotoluene
DL-DOPA
MOLYBDENUM (VI) TETRACHLORIDE OXIDE
5-Norbornene-2-Carboxaldehyde
2,6-Difluoro-4-Methoxybenzonitrile, 97%
6-Fluoronicotinic acid, 97%
2-BroMo-4-nitropyridine
Benzyl disulfide
4-(4-BroMophenyl)-1(2H)-phthalazinone
Diazolidinylurea
4-BroMo-7-chloro-2,8-diMethylquinoline
3,5-DIMETHYLBENZYL ALCOHOL
7-Chloro-4-hydroxyquinoline
2,4-Dichlorobenzene-1-thiol , 97+%
15-Crown-5, 98%
N,N'-Bis(salicylidene)ethylenediamine Iron(II)