NCI-H676B细胞系*人肺腺癌细胞系培养ZX 货号：GD-C15920342
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
传代培养及其操作步骤：原代细胞培养成功以后，需要进行分离培养，否则细胞会因生存空间不足或密度过大，营养障碍，影响细胞生长。细胞由原培养瓶内分离稀释后传到新的培养瓶中培养的过程称之为传代培养。传代细胞允许培养的细胞扩增(形成细胞株)，可以进行细胞克隆，易于保存，但可能丧失一些特殊的细胞和分化特征。传代细胞形成细胞株的利处在于提供了大量持久的实验材料，便于实验。【操作步骤】1)吸掉或倒掉培养瓶内旧培养液；2)向瓶内加入胰蛋白酶液和EDTA混合液少量。以能覆盖培养瓶底为宜；3)置37℃孵箱或室温(25℃温度)下进行消化，2~5min后把培养瓶放在倒置显微镜下进行观察，当发现胞质回缩、细胞间隙增大后，应立即中止消化；4)吸出消化液，向瓶内加入Hanks液少量，轻轻转动培养瓶，把残留消化液冲掉，然后再加培养液。如果仅使用胰蛋白酶消化，在吸除胰蛋白液后，可直接加入少量含血清的培养液，终止消化；5)使用弯头吸管，吸取瓶内培养液，按顺序反复轻轻吹打瓶壁细胞系*使之从瓶壁脱离形成细胞悬液。吹打时动作要轻柔，以防用力过猛损伤细胞；6)用计数板计数后，分别接种于新的培养瓶中，置CO2培养箱中进行培养；7)细胞培养换液时间应根据细胞生长的状态和实验要求来确定。一般2～3d后应换一次生长液。待细胞铺满器皿底面，即可使用；也可继续传代扩大培养或换成维持液。【注意事项】1)掌握好细胞消化的时间，消化时间过短时，细胞不宜从瓶壁脱落，过长的消化会导致细胞脱落、损伤；2)掌握好消化浓度，当消化液浓度过高时，消化时间应缩短，过低时细胞消化时间相对延长。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-Nitrobenzenesulfonyl chloride, 97%
Benzyl (S,S,S)-2-azabicyclo[3.3.0]octane-3-carboxylate hydrochloride
4-ForMylfuran-2-boronic acid
N,N'-Di-2-naphthyl-1,4-phenylenediaMine , 96%
2-BROMO-5-NITROTOLUENE
2-(2-(tert-ButyldiMethylsilyloxy)ethyl)phenylboronic acid
4-(3-Methylbutanoyl)phenylboronic acid
Phenylselenocyanate, tech., 90%
(R)-4-Formyl-2,2-dimethyl-oxazolidine-3-carboxylicacid tert-butyl ester
2-(chloromethyl)-4-methoxypyridine(SALTDATA: 1.02HCl 0.19H2O)
5-Methyl-2-nitrobenzaldehyde
Boc-L-Glutamic acid
Phosmet
BENZOYL BROMIDE
trans-Ethyl cinnaMate
2-BroMo-1-(1-Methyl-1H-benziMidazol-2-yl)-1-ethanone
(1R,2R)-N,N'-Dihydroxy-N,N'-bis(diphenylacetyl)cyclohexane-1,2-diaMine
2,4-DiMethylaniline-5-sulfonic Acid SodiuM Salt Hydrate
Undecylbenzene, 99%
SodiuM Hypochlorite Solution, 5 Percent Available Chlorine, 5 Percent Available Chlorine, Solution, Reagent, ACS
4-(TrifluoroMethoxy)phenol
Diethyl fumarate
2-Chloro-1-phenyl-1-propanone
4-Chlorostyrene
4-fluoro-3-iodotoluene
2,7-Dichlorofluorene
4-Ethylaniline
2-AMINO-6-HYDROXYPYRIDINE
5-Chloro-3-Methylbenzo[b]thiophene, 97%
6-Cyano-2-fluorobenzeneboronic acid pinacol ester, 96%
2-AMino-5-Methoxybenzoic acid
3,5,5-TriMethylhexanoic Acid
N-2-TrifluoroMethylphenyl 4-boronobenzaMide
PERFLUORO-2,5,8,11-TETRAMETHYL-3,6,9,12-TETRAOXAPENTADECANOYL FLUORIDE
N-EPSILON-FORMYL-L-LYSINE
CHLORODIMETHYL-3,3,3-TRIFLUOROPROPYLSILANE
4-Bromo-1,1,2-trifluorobut-1-ene 98%
6-Chloropyridazine-3-carbonitrile
2-Amino-6-chloronicotinic acid
N-(4-Chloropyridin-2-yl)acetamide ,97%
Brompheniramine
(S)-(-)-2,2'-Bis(diphenylphosphino)-1,1'-binaphthyl
4-Fluoro-3-nitrophenylboronic acid
EC 3.2.1.31
5-BroMo-2-picoline
Sulfobetaine 8
2,4,6,8-TetrachloropyriMido[5,4-d]pyriMidine
2-BroMo-6-Methoxynaphthalene, 98%
(S)-3-AMino-3-phenylpropan-1-ol, 95%, 98% ee
1-Phenethyl-4-piperidone
4'-Hexylacetophenone
Ferrosoferric oxide
Reactive Scarlet GF
Sodium 3-(benzothiazol-2-ylthio)-1-propanesulfonate
2-phenyl-1H-imidazole-1-propiononitrile
1,2-Bis(2-Nitrophenoxy)ethane
3-Nitro-N-Methylaniline
3-AMino-3-(2-nitrophenyl)propanoic acid
2,3-DiMethylbroMobenzene
2,4-Difluorophenol
1-(p-Toluenesulfonyl)pyrrole
(+)-Bicuculline
4-Phenylcinnamic acid
2-Aminofluorene
3-Cyano-4,6-dimethyl-2-hydroxypyridine
Potassium chloroplatinate
2,2-Dimethylbutyric acid
4-Iodobenzoyl chloride
1-Benzyl-3-(tert-butoxycarbonylaMino)pyrrolidine
Ethyl 5-chloro-8-Methyl-4-oxo-1,4-dihydroquinoline-3-carboxylate
Allylboronic acid pinacol ester, 98%
O,O-Bis(diethoxyphosphoryl)calix[4]arene, 97%
5-Iodosalicylic Acid
2-BroMo-6-(trifluoroMethyl)pyridine
2-Tridecanone
3-methylazetidin-3-ol
2,3,5,6-Tetrafluoro-1,4-benzenedimethanamine
2-methylpyridine-4-carbaldehyde
GSK ELISA Kit
TAU ELISA Kit
Human apoprotein A1，apo-A1 ELISA Kit
Human ARSE ELISA Kit
Chicken Interleukin 2,IL-2 ELISA Kit
α2-MG ELISA Kit
PGC-1α ELISA Kit
Human monocyte chemotactic protein 3,MCP-3 ELISA Kit
Human VASH2 ELISA Kit
HMG-CoA ELISA Kit
Bromcresol Purple Glucose Peptone Water Medium
Zobell Marine Agar 2216
Iron yttriuM oxide, 99.9% (REO)
PotassiuM Hydrogen Fluoride
5-ALPHA-ANDROSTANE
2-Fluoropyridine-5-boronic acid
Diethyl fumarate
2-phenylbutyrophenone
NBD chloride
3-fluorobenzyl alcohol
5-Methylisoxazole-3-carboxylic acid
3-Hydroxy-4-nitrobenzoic acid
TelluriuM(IV) broMide, 99.9% (Metals basis)
1-(TrifluoroMethyl)vinyl acetate, 97%
3-(TrifluoroMethyl)crotonic acid, 96%
HydroxypropylMethylcellulose
o-ChlorobenzenesulfonaMide
4-Methyl-2-nitrophenylboronic acid
DiMethylsulfaMoyl chloride
CHLOROMETHYL P-TOLYL SULFIDE
trans-Bis(dicyclohexylaMine)palladiuM(II) acetate
Metoclopramide hydrochloride ,99%
2-Thiophenemethanol, 99+%
Dimethylammonium dichlorotri(?chloro)bis{(R)-(+)-2,2'-bis[di(3,5-xylyl)phosphino]-1,1'-binaphthyl}diruthenate(II) [NH2Me2][{RuCl((R)-xylbinap)}2(?Cl)3]
3-Fluoroazetidine hydrochloride
