Bcap-37细胞，人乳腺癌细胞系多久传代 货号：GD-C14119143
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞，全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3,4-Dichlorobenzenesulfonyl chloride, 98%
1-Tetradecene
OCTYL BUTYRATE
3-HydroxypicolinaMide , 98%
TRANS,TRANS-2,4-HEXADIEN-1-OL
Hexa-O-acetyl-cellobial
2-BroMo-4-chlorobenzoic acid
4-Hydroxy-2-phenyl-5-pyrimidinecarboxylic acid
2-(3-phenyl-1,2,4-oxadiazol-5-yl)benzoic acid(SALTDATA: FREE)
1,6-Diphenyl-1,6-hexanedione
Phenyl Isobutyrate
ETHYL HEPTAFLUOROBUTYRATE
Thymol
1,1'-OXALYLDIIMIDAZOLE
2-Methoxy-5-Methylphenyl isothiocyanate
Isophorone
Diisopropyl (BroMoMethyl)boronate
Valeryl BroMide
Tris(diMethylaMino)Methane, 85%
Silver Sulfate, Reagent, ACS
Congo Red
4-Chlorobenzonitrile
2,3-dihydroxy-5-methylbenzoic acid
Dichloro(2-cyanoethyl)methylsilane
2-Amino-5-hydroxybenzoic acid
Isopropyl palmitate
4-IODOPENTYLBENZENE
2,5-DichloronicotinaMide
2,2-Difluoro-2-(fluorosulfonyl)acetic acid, 94%
Ethyl 4-aMinopiperidine-1-carboxylate, 98%
2-Ethoxyethyl acetate
Ethyl 4,4,4-trifluoroacetoacetate
Methyl cyclohexanecarboxylate
Phenyl N-Benzyl-2-aMino-4,6-O-benzylidene-2-N,3-O-carbonyl-2-deoxy-1-thio-beta-D-glucopyranoside , 97%
2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,9-HeptadecafluorononylaMine
2-AcetaMidophenol
1-(Benzo[b]thiophen-2-yl)-2-bromoethan-1-one
3,6-Dichloro-[1,2,4]triazolo[4,3-b]pyridazine
N-(2-Methoxyethyl)ethylamine
n-Octylpentamethyldisiloxane
2-(CHLOROMETHYL)TETRAHYDROPYRAN
1-FLUOROPYRIDINIUM TETRAFLUOROBORATE
Dibromomethane
Hexachlorobenzene;Anticarie;Bunt-cure;Bunt-no-More;
Decahydro-2-naphthol
Iron(II) chloride
Methyl Phenylsulfonylacetate
(S)-(+)-2-ChloroMandelic acid, 99%
1,3-Dichloro-2-iodo-5-(trifluoroMethyl)benzene, 97%
5-METHYLCYTOSINE HYDROCHLORIDE
Thiazolidine-2-carboxylic Acid
7-(diethylamino)coumarin-3-carboxylic acid
4-Ethylphenylboronic acid
2-(METHOXYMETHYL)PYRIDINE
eriocitrin
1,5,9-DECATRIENE
2-[(4-METHOXYBENZYLIDENE)AMINO]FLUORENE
4-Chloro-3-(5,5-diMethyl-1,3,2-dioxaborinan-2-yl)pyridine, HCl
(R)-(-)-3-FLUOROPYRROLIDINE HYDROCHLORIDE
1,2,3-Thiadiazole-4-carbonyl chloride ,97%
Tetrahydrofuran-2,3,4,5-tetracarboxylic acid, 97%
3-Bromobenzaldehyde diethyl acetal, 95%
Mannan from Yeast
1-CHLORO-2-IODOETHANE
Homoarginine
Perflurohexane sulphonyl fluoride
TRIETHYLENE GLYCOL MONOBUTYL ETHER
4-(ChloroMethyl)-2-phenyl-1,3-thiazole
1-AMinoheptadecane
Pyridine-3-sulfonaMide
3-Chlorophenoxyacetic acid, 98%
Manganese(II) chloride tetrahydrofuran coMplex (1:2), 97%
N-Ethylcarbazole
Benzoyl Leuco Methylene Blue
4-Isopropylaniline
1H-Imidazole-4-carboxylic acid, methyl ester, monohydrochloride (9CI)
Reactive Blue 104
dipotassium sulphide
MLT ELISA Kit
Mouse FOS (V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog) ELISA Kit
Human Apo-C3 ELISA Kit
Human DEFβ116 ELISA Kit
ALCAM ELISA Kit
MYC ELISA Kit
Hen egg phosvitin phosphopeptides,PPP ELISA Kit
MAO ELISA Kit
Human ANGPTL3 ELISA Kit
CH50 ELISA Kit
Modified Mycoplasma Agar Medium Base（Frey）
Antibiotic Examination Medium Ⅱ
YtterbiuM(III) isopropoxide
Ethyl hydrogen fuMarate, 97%
Methyl 5-chloro-2-nitrobenzoate, 98%
2-Fluoro-5-nitrobenzeneboronic acid pinacol ester, 96%
4-Chlorobenzonitrile
Vat Brown 68
amberjet 4400 oh strongly basic anion*exchanger
2,6-bis(trifluoromethyl)pyridine
Tridecanal
TRIETHYLGERMANIUM CHLORIDE
3-Methyl-2-Buten-1-ol
3-(BenzyloxyMethyl)cyclobutanone, 97%
N-Benzyloxycarbonyl-D-phenylalaninol, 98%
2-BroMo-1-indanone
6-Methoxy-2-(4-Methoxyphenyl)?benzo[b]thiophene
(2S,3S)(-)-Dihydroxybutane-1,4-dioic acid diethyl ester
2-Azidobenzoic acid solution
4-Acetoxy-3-Methoxyphenylboronic acid, pinacol ester
4-(2-Chlorobenzyloxy)-3,5-diMethylphenylboronic acid
1,3-Bis(diphenylphosphino)propane, 98% DPPP
Doyle dirhodium catalyst - RH2(4S-MPPIM)4
2-Ethynylnaphthalene
(S)-(+)-4,12-Bis(di(3,5-xylyl)phosphino)-[2.2]-paracyclophane, min. 93% CTH-(S)-3,5-xylyl-PHANEPHOS
