基质胶 高浓度 低生长因子

Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.SourceEngelbreth-Holm-Swarm Mouse TumorQuality• Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing• Extensive PCR testing is performed to screen for a number of pathogens• Tested and found negative for bacteria, fungi, and mycoplasma• Protein concentrations determined by Lowry method• Endotoxin units measured by Limulus Amoebocyte Lysate assay• Matrigel Matrix gel stability is tested for a period of 14 days at 37°C• Biological activity is determined for each lot using a neurite outgrowth assayPreparation and StorageStore at -20°C. Avoid multiple freeze-thaws.Application• Cell Growth and Differentiation• Metabolism/Toxicology Studies• Invasion Assays• in vitro and in vivo Angiogenesis Assays• in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice