WEHI 231细胞系——小鼠B淋巴细胞系如何传代

WEHI 231细胞系——小鼠B淋巴细胞系如何传代 货号：GD-C11419225

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞，全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
(R)-(-)-2-Methyl-2,4-pentanediol, 98+%
6-Methyl-2-pyridineMethanol
2-[2-[2-[2-[2-[2-(BENZYLOXY)ETHOXY]ETHOXY]ETHOXY]ETHOXY]ETHOXY]ETHANOL
Acid Red 87
3-(4-Methylphenoxy)benzaldehyde
DL-ALPHA-AMINOSUBERIC ACID
4-N-PROPYLPIPERIDINE
9,9-Dioctylfluorene-2,7-diboronic acid, 98%
3-oxa-9-azaspiro[5.5]undecane(SALTDATA: FREE)
Tributylphosphine oxide, 98%
2-(Trimethylsilyl)phenyl Trifluoromethanesulfonate
1,3-Phenylenediacetonitrile
2-NONANOL
Terbutaline
DL-Phenylalanine
5-(2-Furyl)thiophene-2-carbaldehyde
Diphenyl alpha-Chlorobenzylphosphonate
Methyl 8-broMo-4,6-dichloroquinoline-2-carboxylate
Xylenol Orange, sodiuM salt, pure, water soluble
Sorbitan MonopalMitate, NF, EP
TetrahexylaMMoniuM Iodide
myristyl acetate
6-Aminothymol hydrochloride
ethyl 5-methyl-3-phenylisoxazole-4-carboxylate
4-fluorobenzhydrazide
4-Amino-3,5-dichloropyridine
Shikimic acid
2-BroMo-3-Hexylthiophene
Hexachloropropene, 95%
5-Hydroxyisophthalic acid, 98%
Glycine benzyl ester hydrochloride
Cupric Acetate Anhydrous
N-Cyclopropyl 4-fluorobenzaMide
Oxobutanedioic acid
2-Isopropoxyethyl Benzoate , 90%
5-AcetylaMido-2-chloroaniline
Sodium Diphenylphosphinobenzene-3-sulfonate
2,3-Difluoroanisole
Potassium Gluconate
Ethyl pyrrolidinoacetate, 98%
Ninhydrin hydrate
Benzidine
TRIMETHYLENE SULFIDE
3-(Decyldimethylazaniumyl)propane-1-sulfonate
n-Butyrophenone
CyclobutylaMine hydrochloride
delta-Tridecanolactone
2,5-Difluorophenol, 96%
(+)-DiMethyl 2,3-O-isopropylidene-D-tartrate, 97%
YTTRIUM NITRATE
DiMethyl(Methylthio)sulfoniuM Tetrafluoroborate
pyriproxyfen
N,N-Bis[3-D-gluconamidopropyl]-deoxycholamide
Methoxyacetyl chloride
mianserin hydrochloride
4-(Trifluoromethylthio)aniline
Methyl o-toluate
[(4R)-2,2-DiMethyl-5-oxo-1,3-dioxolan-4-yl]acetic Acid
4,10-Dioxatricyclo[5.2.1.0(2,6)]decan-8-en-3-one , 98%
3-Cyanobenzyl chloride
2,4-Dimethoxybenzoyl chloride, 98%
1,1-Dimethylsila-14-crown-5
4-(Dimethylamino)-2-methylbenzaldehyde
Ethyl 3-aminopropanoate hydrochloride
2-(4-CHLOROPHENYL)ETHYL ISOTHIOCYANATE
EDTA FERRIC-SODIUM SALT
2-Chloroanisole
4-Methyl-3-nitroaniline
1-BroMo-2-naphthaldehyde
DiMethyl 3,3'-bipyridine-6,6'-dicarboxylate
Androsterone, 97%
Butyl glycidyl ether, 95%
N,N'-Diisopropyl-O-(4-nitrobenzyl)isourea [for HPLC Labeling]
D-Valine Benzyl Ester p-Toluenesulfonate
ethyl-2-thiourea
5-Pyrimidinecarboxylic acid, 2-(trifluoromethyl)-
5-bromo-2,3-dichloropyridine
Cimicifugoside
Porcine myelin basic protein,MBP ELISA Kit
SFRS2IP/CASP11 ELISA Kit
FBN2 ELISA Kit
Human UBD ELISA Kit
Chicken β-lactamase ELISA Kit
Rat α-KGDHC (α Ketoglutarate Dehydrogenase) ELISA Kit
Chicken Thyroxine,T4 ELISA Kit
Human ChAT ELISA Kit
Human HO2 (Heme Oxygenase 2) ELISA Kit
IA-2A/PTP ELISA Kit
No.4 Agar Base
Yeast Extract Agar
Methyl Acetoacetate
AntiMony(V) fluoride coMpound with graphite, 1:1 Mixture
5,6-Diphenyl-3-(2-pyridyl)-1,2,4-triazine-4,4'-disulfonic acid disodiuM salt hydrate, 98%
2-Fluoro-4-Methoxybenzyl alcohol, 97%
myristyl acetate
1,4-bis(4-methyl-α-styryl)benzene
2,7-DI-TERT-BUTYLNAPHTHALENE
3-Fluorobenzyl cyanide

1-BROMO-5-METHYLHEXANE
D-Pyrrolidine-2-carboxylic acid
t-Butyl 2-broMo isobutyrate
2-BroMo-5-(trifluoroMethoxy)phenol, 97%
Tetrahydrofurfuryl acetate, 98%
cis-4,7,10,13,16,19-Docosahexaenoic acid ethyl ester
3,4′-Oxydianiline
N-METHOXYDIACETAMIDE
Methyl 2-Methylglycidate
6-(DiMethylaMino)fulvene , 98%
Manganese(II) fluoride, 99%
Pentasodium Diethylenetriaminepentaacetate (ca. 40% in Water, ca. 1.0mol/L)
Potassium ferricyanide, for analysis ACS, 99+%
3',5'-Dichloro-2,2,2-trifluoroacetophenone
2-Amino-3-methylquinoxaline ,97%