Kit225-k6细胞系——人T淋巴细胞系如何传代 货号:GD-C11419199
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Nitrobenzene, 99%
D-(+)-Cellobiose
EthinaMide
N-[3-(TriMethoxysilyl)propyl]ethylenediaMine
Diamond powder
DICHLORODI-N-PENTYLSILANE
Diethyl trans-cinnaMylphosphonate
2,6-Dimethoxyphenol
Biotinyl-6-aminoquinoline
Methyl 2,3,6-Tri-O-benzoyl-alpha-D-galactopyranoside
Bis(4-chlorobutyl) Ether
3-Fluoro-4-methylbenzonitrile
2,4-Thiazolidinedione
N-Phenylbenzylamine
Tri(p-tolyl)phosphine
Zinc
DibroMoisocyanuric Acid
ZonisaMide
VanadiuM(IV) chloride, 99+%
SodiuM Polyphosphates, Glassy, FCC
Difluoroacetic Anhydride
Tetrahydrofurfuryl acetate
Potassium tetraoxalate dihydrate
7-Formylindole
α,α,α-trifluoro-4-iodotoluene
1-Methyl-4-piperidone
BUPIRIMATE
Ligninesulfonic Acid SodiuM Salt
N-FMoc-L-glutaMic acid 5-benzyl ester, 95%
2-Fluoro-4-nitrophenol, 98+%
H-D-Aspartic acid-β-Methyl ester
trans-1,2-DiaMinocyclohexane-N,N,N′,N′-tetraacetic acid Monohydrate
N, N-Diisopropyl 2-broMo-5-fluorobenzaMide
PENTAERYTHRITOL DIOLEATE
4-FLUORO-2-IODOTOLUENE
7-Methylindole
2-Fluoro-5-hydroxymethylpyridine
Dilauroyl peroxide, 99%
(pyrazolo[1,5-a]pyridin-3-ylmethyl)amine(SALTDATA: FREE)
(S)-3-Amino-1-carbobenzoxypyrrolidine Hydrochloride
4-Chlorobenzyl Mercaptan
N-FLUORO-4-METHYLPYRIDINIUM-2-SULFONATE
Aluminium glycinate
Ethylthiocyanate
Tritolyl phosphate
1,6-Diphenyl-1,3,5-hexatriene
(S)-(-)-6,7-DiMethoxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic Acid Hydrochloride
4-(DiMethoxyMethyl)pyriMidine
5-BroMo-1H-benziMidazole, 97%
1,4-DINITROBENZENE
1-Benzoyl-4-piperidone
N-Butaldimethylchlorosilane
Isoquinoline-4-carboxylic acid
Diethyldithiocarbamic acid diethylammonium salt
2-Methylacetophenone
Gluconic acid
BroMochlorophenol Blue
Ethyl 5-(2,4-dichlorophenyl)oxazole-4-carboxylate
2'-BENZYL-2,2-DIMETHYLPROPIONANILIDE
2-Chloro-4,5-dimethoxybenzoic acid
Phenylselenenyl Bromide
4-Bromo-N,N-diethylbenzamide
2,4-Dinitrobenzenesulfonic Acid Hydrate
N'-HYDROXYPYRIDINE-4-CARBOXIMIDAMIDE
Indoline
5-ACETYL-2-FLUOROPHENYLBORONIC ACID
Zidovudine
4-Chloro-2-(trifluoroMethyl)aniline
1-BroMo-1-propene (cis- and trans- Mixture)
2-(BroMoMethyl)-5-fluorophenylboronic acid, pinacol ester
8-Hydroxyquinoline, ACS reagent
2,4,5-TriMethoxybenzaldehyde, 98%
2-Fluoro-6-Methylpyridine
L-Selenocystine
3-iodopropene
2-Iodopyridin-3-ylamine
3,4-Dichlorobenzoic Anhydride
6-chlorobenzo[d]isoxazol-3-ol
PC ELISA Kit
Mouse PIAS1 (Protein Inhibitor Of Activated STAT 1) ELISA Kit
Human PIBF1 ELISA Kit
Human UBE2A ELISA Kit
IL-15 ELISA Kit
IFNα ELISA Kit
ACV-B ELISA Kit
CH ELISA Kit
Human HBζ ELISA Kit
NO ELISA Kit
Strain Medium
Bromcresol Purple Glucose Peptone Water Medium
Ethoxyquin
FLURBIPROFEN
5,7-DICHLOROKYNURENIC ACID SODIUM SALT
2-Fluoro-4-nitroanisole, 95%
Tetrahydrofurfuryl acetate
Sodium 2,2',2'',2'''-(1,2-ethanediyldinitrilo)tetraacetate hydrate (4:1:4)
4-(2-methoxyethyl)morpholine
4-Fluorobenzaldehyde
N-BENZOYL-4-PIPERIDONE
SODIUM COBALTINITRITE
5-BroMo-2-Cyano-3-Nitro Pyridine
Hexafluoro-2-(p-tolyl)-2-propanol, 97%
n-Octacosane, 99%
Diethyl dithio carbaMic acid silver salt
IsoMaltitol
4-(N-HydroxycarbaMiMidoyl)phenylboronic acid
L-Phenylalanine benzyl ester hydrochloride
Ethyl p-nitrobenzoate
CYCLOHEXANE-1 2 4 5-TETRACARBOXYLIC ACI&
Tetraethyl orthocarbonate, 97%
Alendronate sodium
3-(methoxymethyl)piperidine(SALTDATA: HCl)
Hydantoin, 99%