3T3 L1细胞|小鼠胚胎成纤维细胞系什么细胞 货号：GD-C10520500
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞，全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Copper BerylliuM rod, alloy C17200, 4.76MM (0.188in) dia
(MethylaMino)acetaldehyde diMethyl acetal
6-Chloro-2-(tributylstannyl)pyridine
3-Chloro-2-Methoxyphenylboronic acid
4',6'-DiMethoxy-2'-hydroxyacetophenone , 98%
Methylboronic acid
3,5-DibroMo-4-hydroxybenzaldehyde
2-Chloro-5-fluorobenzylamine
1,1'-Thiocarbonyldiimidazole
(3-Bromopyridin-4-yl)methanol
8-Hydroxyquinoline-5-sulfonic acid monohydrate, 98%
Formamidine acetate
5,6-Diaminonaphthalene-1,3-disulphonic acid
Zofenopril calcium
1,3-DibroMobenzene
Trans-4-AMinocyclohexanol
1,1-Diisopropoxycyclohexane
Methyl 2-broMo-5-(trifluoroMethoxy)benzoate
Benzyl 4-[(MethylaMino)Methyl]piperidine-1-carboxylate, 95%
Thioridazine Hydrochloride, USP
Tris(2-butoxyethyl) Phosphate
1-Methyl-3-(trifluoromethoxy)benzene
2,9,10-trimethoxy-5,13a-dihydro-6H-isoquino[3,2-a]isoquinolin-3-ol
Copper(II) sulfide
Homo
(Z)-2-Amino-alpha-[1-(tert-butoxycarbonyl)]-1-methylethoxyimino-4-thiazolacetic acid
Lithium hydroxide
3,4-Dichlorobenzyl Alcohol
5-BroMo-1-pentene, 96%
3,5-Diacetoxybenzoic acid, 97%
4-(N-[2-(tert-ButyldiMethylsilyloxy)ethyl]sulfaMoyl)benzeneboronic acid, 96%
Benzyl cinnaMate
2-Cyanoethyl N,N,N',N'-tetraisopropylphosphorodiaMidite
N-OCTYL CYANIDE
3-CHLORO-2-HYDROXYPROPYL METHACRYLATE
Dibenzyl oxalate
2-Methoxy-β-nitrostyrene
2,3-Dihydro-1,4-benzodioxin-6-ylmethylamine , 95%
4-(4H-1,2,4-Triazol-4-ylcarbamoyl)benzeneboronic acid hydrochloride 98%
Lithium 3,5-diiodosalicylate, 95%
(4S,5S)-1,3-Dimethyl-4,5-diphenyl-2-[(R)-1-benzyl-2-hydroxyethylimino]imidazolidine
4-Acryloylmorpholine
2-(4-METHYLPIPERAZINO)BENZALDEHYDE
2-(3-Chlorophenoxy)-propionic acid
5-Chloro-2-nitroaniline
2-Hydrazinopyridine
(-)-1,4-Di-O-tosyl-2,3-O-isopropylidene-L-threitol
5-BroMo-8-Methoxy-2-Methylquinoline
Polycarbonate resin, approx. M.W. 45.000, pellets
Flunixin MegluMine, USP
(1alpha,3alpha,5alpha)-1,3,5-Cyclohexanetriol
N-phenyl-2-naphthylamine
9H-Purin-2-amine, 6-chloro-9-methyl-
naphthol as-bi-phosphate disodium salt heptahydrate
Linalool
2,5-DIFLUOROPHENYL ISOTHIOCYANATE
2-AMino-7-broMo-5-oxo-5H-[1]benzopyrano[2,3-b]pyridine-3-carbonitrile
3-SULFOBENZOIC ACID DISODIUM SALT MONOHYDRATE
2,6-Difluoro-3-Methoxybenzoic acid, 97%
(4R,5R)-(-)-2,2-Dimethyl-a,a,a',a'-tetra(1-naphthyl)-1,3-dioxolane-4,5-dimethanol, min. 97% (R,R)-1-Nph-TADDOL
Boc-trans-4-Fluoro-L-proline
4,4'-Dinonyl-2,2'-bipyridyl
Boc-(2S,4S)-4-phenyl-pyrrolidine-2-carboxylic acid
4-Chloromandelic acid
2-Amino-6-chlorobenzonitrile
2,4-Dichloro-6-methylpyrimidine
INDIUM ACETYLACETONATE
3,4-DiMethylthieno[2,3-b]thiophene-2-carbaldehyde
1-Cyanovinyl Acetate (stabilized with TBC)
3-Chloro-5-nitropyridin-4-ol
4,4'-DiMethoxybenzophenone, 97%
2-(Methyldithio)isobutyraldehyde, 95%
Thymol
1-Methyl-4-piperidineMethanol
4-n-Butoxyphenol
(S)-2-Aminomethyl-1-N-Cbz-Pyrrolidine
2,3,5-trifluorobenzyl alcohol
2,3-Dimethylcyclohexylamine, mixture of isomers
GHRP-Ghrelin ELISA Kit
Mouse N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA Kit
Human Free Prostate Specific Antigen,fPSA ELISA Kit
DAO ELISA Kit
E2 ELISA Kit
Rat beta cellulin,BTC ELISA Kit
NRG1 ELISA Kit
Human protein kinase B,PKB ELISA Kit
Human PECAM1 ELISA Kit
MUC7 ELISA Kit
CATC Agar
Azide Dextrose Broth
AcetaMide
Flumethrin
5-(2-BroMophenyl)-1H-tetrazole, 98+%
1-Cyano-2-Iodobenzene
1-Methyl-3-(trifluoromethoxy)benzene
Ethyl (4-methylthiophenyl)acetate
Trichlorobenzene
alpha,alpha'-diamino-P-xylene
MERCURIC PERCHLORATE
(1S,2S)-(-)-1,2-Diphenyl-1,2-ethanediamine
L(-)-2-AMino-3-Phenyl-1-Propanol
N^a-Benzyloxycarbonyl-O-tert-butyl-L-serine, 98%
SulfaMethazine, 99%
4-Chloro-2-(trifluoroMethyl)quinoline
2-(TrifluoroMethoxy)benzaldehyde
2-Methoxy-5-trifluoroMethylpyridine-3-boronic acid
1-N-BUTYL-4-(ETHYLTHIO)BENZENE
[(3S,8S,10R,13R,14S,17R)-10,13-diMethyl-17-[(2R)-6-Methylheptan-2-yl]-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-yl] 2,4-dichlorobenzoate
2-BENZYL-2-IMIDAZOLINE HYDROCHLORIDE
2-Bromo-4-chlorothieno[3,2-c]pyridine
3-Benzyloxybenzhydrazide
4-Piperidinobenzaldehyde, 97%
2-Methyl-4-(trifluoromethoxy)benzeneboronic acid 98%
