HePa1-6细胞株|小鼠肝癌细胞系技术优势大 货号：GD-C9919295
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞，全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Triethoxysilane, 96%
VacuuM puMp oil
2,5-PYRIDINEDICARBOXYLIC ACID DI-N-PROPYL ESTER
N-Isopropyl 2-broMobenzaMide
DIETHYLAMMONIUM DIETHYLDITHIOCARBAMATE
Pentaerythritol triacrylate
2-Methoxycarbonylphenylboronic acid, pinacol ester
3-Fluoro-5-(trifluoromethyl)benzoic acid
Cyclobutyl Phenyl Ketone
4-Methylheptane
Gallion
Styrene
5-Amino-2-fluorobenzonitrile
2,4-Dichlorophenoxybutyric acid
Chloroacetyl chloride
N,N,N',N'-TetraethylethylenediaMine
DiMethyl 1,3-Benzodithiol-2-ylphosphonate
N-Methyl 3-broMo-5-nitrobenzaMide
Methyl 4-(4,4,5,5-tetraMethyl-1,3,2-dioxaborolan-2-yl)benzoate, 97%
Terbinafine Hydrochloride, EP
N,N'-Bis(2-aMinobenzal)ethylenediaMine
Pyrrolidinodithiocarbamicacidallylester
Acid Yellow 17
tetraethylammonium acetate tetrahydrate
Cycloheptanone
Hexadecanethiol
4-Aminothiophenol
Aniline Hydrochloride
2-Pyridylzinc broMide, 0.5M in THF, packaged under Argon in resealable CheMSeal^t bottles
[1,1'-Bis(diphenylphosphino)ferrocene]tetracarbonyltungsten, 98%
ThioseMicarbazide
2-Chloro-6-fluorobenzoic Acid
AMBERLITE IR-120
N-t-butyl-4-fluorobenzaMide
3-Methyl-1,2-cyclopentanedione
(S)-1-[(RP)-2-[Bis(4-Methoxy-3,5-diMethylphenyl)phosphino]ferrocenyl}ethyldi-tert-butylphosphine
2-Chloroethyl Phenyl Sulfone
2,4,5-Trifluorobenzyl chloride
3-Amino-5-methylisoxazole
2-Ethylhexyl Butyrate
1,2-Dibromo-3-chloropropane
SILVER BROMIDE
3-Heptylacrolein
1-VinyliMidazole
Glutaronitrile
2-AcetaMido-6-nitrobenzoic Acid
3,4-Dihydro-2H-isoquinolin-1-one
4-BroMo-1,2-(Methylenedioxy)benzene, 97%
Cholesteryl 2-ethylhexanoate
Benzyladenine
Acid Anthracene Brown PG
Benzyl acetate
FMOC-LYS(BIOTIN)-OH
3-Iodobenzonitrile
1-Methyl-3-pyrrolidinecarboxylic acid hydrochloride
N-METHYLANTHRANILIC ACID
5-Chloro-2-fluoro-4-Methylphenylboronic acid
4-MethoxytriphenylaMine
RubidiuM hydroxide hydrate, 99% (Metals basis)
3-Chloro-2-fluoroaniline
Dansyl-L-valine
2-Amino-5-(trifluoromethyl)phenol 97%
Methyl 4-Methylsalicylate
4-IODOBENZALDEHYDE
2-Phenylbenzothiazole
Ethyl 4-[[(ethylphenylamino)methylene]amino]benzoate
Rhodamine 101 chloride
CesiuM hydroxide
1-Butyl-3-MethylpyridiniuM BroMide
2,4-Dichloro-5-propoxyphenylboronic acid
Copper(II) acetylacetonate, 98%
1-(4-Acetylpiperidino)ethan-1-one, 97%
6,6-Diphenylfulvene
ThiopropionaMide
hydroxytrimethylsilane
5-bromo-2,4-dichloro-pyridine
Acid Brown 432
5-Hydroxy-1-naphthalenesulfonic acid sodium salt
Eotaxin 1 ELISA Kit
Podocin ELISA Kit
Human Protamine 1,PRM1 ELISA Kit
Human DPYD ELISA Kit
Chicken 1,3-βD glucosidase,1,3-βD-Glu ELISA Kit
Rat βMSH (beta-Melanocyte Stimulating Hormone) ELISA Kit
IgM ELISA Kit
Human PARK7 ELISA Kit
CH50 ELISA Kit
PRM 1 ELISA Kit
Sodium malonate Medium
MR-VP Medium
RhodiuM(III) 2,4-pentanedionate, PreMion|r, 99.99% (Metals basis), Rh 25.2% Min
Calcium fluoride
5-(o-Tolyl)-1,3,4-oxadiazole-2-thiol, 96%
DI-2-Pyridyl Ketone
Pyrrolidinodithiocarbamicacidallylester
m-phenylenediamine hydrochloride
Nickel Hydroxide
Triacontanoic acid
4-BROMO-2,6-DIMETHYLANISOLE
trans-Zeatin-riboside
Maltitol
9-Fluorenone oxiMe, 98+%
Hexanal, 98%
IsoaMyl Isovalerate
VitaMin B12
4,6-O-Ethylidene-alpha-D-glucose
2-Methyl-2-adaMantanol
3-(1-Naphthyl)acrylic acid
INDOPHENOL BLUE
Vinyl crotonate, 98%
3,4-Dimethylheptane
Nitroethane, 98+%
(4-methoxyphenyl)(methylamino)acetic acid(SALTDATA: FREE)
