HUV-EC-C[HUVEC]细胞株|人脐静脉内皮细胞系行业低价 货号：GD-C9320477
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞，全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Allyl acrylate, tech. 90%, stab. with ca 100ppM 4-Methoxyphenol
Fluorene-2-carboxaldehyde
4-(2-HYDROXYETHYL)-3-METHYL-1-PHENYL-2-PYRAZOLIN-5-ONE
2-(Acetylthio)norbornane, Mixture of isoMers
4-(TRIFLUOROMETHOXY)BENZYLAMINE
N-METHYLBENZENESULFONAMIDE
Methyl 5-chloropyrazine-2-carboxylate
Acetylsalicylic acid ,99%
Indium phosphide (99.999%-In) PURATREM
Methyl 2-oxindole-5-carboxylate 96%
N-Methoxy-N-methyl-4-(trifluoromethyl)benzamide
N,N'-BIS(SALICYLIDENE)-1,2-PROPANEDIAMINE
2-(PERFLUOROPROPOXY)PERFLUOROPROPYL TRIFLUOROVINYL ETHER
2-Amino-4'-chloroacetophenone
D-Arginine
4-Iodo-5-Methyl-3-phenylisoxazole
3,4-DibroMothieno[2,3-b]thiophene
Methyl 4-broMopicolinate
3-Fluoro-5-(trifluoroMethyl)phenylboronic acid, 97%
Tetracaine, USP
N-(2,6-DiethylphenylcarbaMoylMethyl)iMinodiacetic Acid
2-Hydroxy-6-methoxyacetophenone
3,4-Diiodopyrazole
Ytterbium(III) oxide
1,3-Cyclohexanedione
1,2-Diaminopropane-N,N,N',N'-tetraacetic acid
RAC-ETHYLENEBIS(4,5,6,7-TETRAHYDRO-1-INDENYL)ZIRCONIUM DICHLORIDE
2-Chloro-3-Fluoro-BenzylaMine
Zinc titaniuM oxide, 99.9% (Metals basis)
1,3-Cyclopentanedione, 98%
Methyl acrylate, 99%, stab. with ca 15ppM 4-Methoxyphenol
Dibutyl Adipate
3-BroMo-2-isopropoxy-5-forMylphenylboronic acid
N-PHENYL-THIOBENZAMIDE
Methyl 6-fluorochroMone-2-carboxylate
TRIBUTYLPHENYLTIN
4-Fluoro-2-MethoxybenzylaMine, 97%
3-Hydroxypropionic Acid (contains varying amounts of 3,3'-Oxydipropionic Acid) (ca. 30% in Water, ca. 3.6mol/L)
Ethyl 4,4-difluoro-2-(ethoxymethylene)-3-oxobutanoate
1-(3-Bromo-4-methoxyphenyl)ethanone
(4S)-4-Benzyl-L-glutamic acid
DL-NORADRENALINE HYDROCHLORIDE
AZOXYBENZENE
3-AMINO-2,6-DIMETHYLPYRIDINE
5-Chloro-2-nitro-4-(trifluoroMethyl)aniline
4,6-DiMethyl-2-MercaptopyriMidine
Diethyl (4-Isopropylbenzyl)phosphonate
N-Ethyl 2-broMobenzaMide
Trichloroacetyl chloride, 97%
PotassiuM Carbonate, Anhydrous, Granular, USP
Diethyl 3-Hydroxyglutarate
2,3-Dibromosuccinic acid
3,4-Diethoxybenzoic acid
1-Methyl-2-pyrrolidinone
2,5-Dinitrophenol
3-Furanboronic acid
1-BIPHENYL-2-YL-N-METHYLMETHYLAMINE
N-(2-Methylphenyl) 2-broMobenzaMide
Oxalic acid dihydrate
N~1~,N~1~-dimethyl-1-(4-methylphenyl)-1,2-ethanediamine(SALTDATA: FREE)
1-Boc-4-vinyl-piperidine
D-(-)-Fructose ,99% [Natural]
(1S,2S)-(+)-2-Amino-1-phenyl-1,3-propanediol, 98%
2,4,5-Trimethylbenzoic acid
Allyl cyanoacetate
Sulfur dichloride
2-Thiophenesulfonyl chloride
Decanal
1-Chloro-2-Methyl-2-phenylpropane
2-Methoxy-2-(1-naphthyl)propionic Acid
N,N-DiMethyl-2-(4-nitrophenoxy)ethanaMine
Benzo[b]selenophene, 97%
(+)-ISOMENTHOL
4,6-Dichloro-2-(Methylthio)pyriMidine
2,5-bis(octyldithio)-1,3,4-thiadiazole
methyl 3-hydroxy-4,5-dimethylthiophene-2-carboxylate
Saponin
Triethoxyethane
ATXN2 ELISA Kit
P27 protein ELISA Kit
FFA ELISA Kit
Human DHLNL ELISA Kit
nNOS ELISA Kit
Rat GUSb (Glucuronidase, Beta) ELISA Kit
HSP-20 ELISA Kit
Human ZPI ELISA Kit
Human TP/TXA2R (Thromboxane Receptor) ELISA Kit
ACF ELISA Kit
LPM Agar with Esculin and Ferric Iron Base
Indole Nitrite Medium
VanadiuM(III) 2,4-pentanedionate, typically 97%
Hexafluorosilicic acid
5-(4-Fluorophenyl)isoxazole, 99%
2-Butynoic acid
2-Hydroxy-6-methoxyacetophenone
4-Chloro-6-nitroquinoline
Yttrium(III)-oxide
trans-4-hydroxy-3-methoxycinnamic acid
Thiazolyl Blue
Betaine
(L)-Ethyl 2-AMino-3-Methylbutanoate Hydrochloride
2,3-DibroMo-5-chloropyridine, 96%
3-Methylcatechol, 98%
Hexythiazox
α-BroMoisobutyryl broMide
(3-BROMO-4,5-DIHYDRO-ISOXAZOL-5-YLMETHYL)-CARBAMIC ACID TERT-BUTYL ESTER
3-(4-BroMophenyl)-5-(4-fluorophenyl)-1,2,4-oxadiazole
1-(5-BroMo-1H-pyrrol-2-yl)-2,2,2-trichloroethanone
SodiuM bis(triMethylsilyl)aMide
tert-Butyl 4-(2-hydroxyethyl)-1-carboxylate , 97%
(4-Hydroxy-2-phenyl-1,3-thiazol-5-yl)acetic acid
Dabsyl-L-leucine
2,5-Dichloroamylamine Hydrochloride
