K7M2 wt[K7M2-WT]细胞株：小鼠骨肉瘤成骨细胞系促销多 货号：GD-C8519089
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
N,N,N',N'-TetraMethyl-1,3-butanediaMine, 99%
Resazurin
ALUMINUM ANTIMONIDE
(+/-)-10-CAMPHORSULFONIC ACID SODIUM SALT
N-Butyl 5-broMonicotinaMide
4-CHLORO-8-METHOXY-2-METHYLQUINOLINE
2,6-DIMETHYLQUINOLINE
2-Methyl-benzothiazole-5-carboxylic acid
Methyl 2,6-dichloro-5-fluoronicotinate, 90%
5-Bromo-2-methoxypyridine-3-boronic acid 97%
2-Phenyl-1-propanol
Ethyl 2-aminothiazole-4-carboxylate hydrochloride
N-epsilon-Boc-D-lysine
Methyl 6-methylnicotinate
N-Carbobenzyloxyglycine
3-Chloro-1H-indazole
4-(DiMethylaMino)butyraldehyde DiMethyl Acetal
N-Isopropyl 3-broMo-5-nitrobenzaMide
Tri-M-tolyl phosphate, 97%
Pyridoxine Hydrochloride, USP
2-Cyanophenylacetonitrile
o-Vinyltoluene
p-Toluenesulfonylacetonitrile
2-Benzothiazolecarbonitrile, 6-methoxy-
adrenosterone
3-OXO-3,4-DIHYDRO-2H-1,4-BENZOXAZINE-6-CARBOXYLIC ACID
Calcium cyanamide
D-(-)-Mandelic Acid Methyl Ester
Methylene Blue, high purity, biological stain
N-(3-Acetyl-4-hydroxyphenyl)butyraMide, 97%
3,5-Difluoro-2-Methylbenzonitrile, 97%
3-Methylcyclopentane-1,2-dione
ACETIC ACID (2R,3R,4R,5R)-3,4-DIACETOXY-5-(6-CHLORO-2-IODO-PURIN-9-YL)-TETRAHYDRO-FURAN-2-YLMETHYL ESTER
PlatinuM tetraMMine hydrogencarbonate
(R)-(+)-2-AMino-1,1,3-triphenyl-1-propanol
5-AMino-4,6-dichloro-2-MethylpyriMidine
1,1'-Dimethylferrocene
4-Aminomethyl-1-N-butylpiperidine
(1S,2R)-2-[N-Benzyl-N-(mesitylenesulfonyl)amino]-1-phenyl-1-propanol
Ethyl 4H-thieno[3,2-b]pyrrole-5-carboxylate
(3,4-dimethoxybenzyl)hydrazine(SALTDATA: FREE)
5-METHYL-2-NITROANILINE
SODIUM OLEATE
2-Methyl-1-pentene
2-[(4-Methylphenyl)thio]nicotinic acid
4-BroMo-N,N-diMethylaniline
N,N-DiMethyloxaMic Acid
2-Chloro-5-Methylpyridine-3-carbaldehyde
2-Mercaptopyridine-N-oxide, sodiuM salt, 40 w/w % aqueous solution
VitaMin A PalMitate, USP
Acetone Dibutyl Acetal
4-methyl-2-oxovaleric acid
Ethyl 2-(bromomethyl)benzoate
N-Benzylbenzamide
Ethylene Mercaptan
4-Benzyloxybenzeneboronic acid
2-Hydroxy-4-(trifluoroMethyl)pyriMidine
D-alpha-AMino-beta-phenylpropionic acid
Boc-(R)-α-(4-tert-butylbenzyl)-Pro-OH
(S)-2-Amino-propan-1-ol
6-Methyl-5-nitroquinoline , 97%
2-Acetyl-1-methylpyrrole
Di-mu-chlorobis[2-[(dimethylamino)methyl]phenyl-C,N]dipalladium(II)
2-ISOPROPYLNAPHTHALENE
2-Bromo-5-methylpyridine
Tazarotene
Arkopal N 100
Diphenylacetyl chloride
12-Crown-4
2-Methoxy-6-MethylaMinopyridine
N,N-Diethyl 4-aMinobenzenesulfonaMide
4-BroMo-1H-indole-3-carboxylic acid, 97%
Nerolidol
Flavianic Acid Dihydrate
4-bromo-1,1,2-trifluorobut-1-ene
3-[Methoxy(dimethyl)silyl]propan-1-amine
7-bromo-1-heptanol
Dicyclohexylmethanol
GKN1 ELISA Kit
Mouse α2-plasmin inhititor,α2-PI ELISA Kit
Human EGR1 (Early Growth Response Protein 1) ELISA Kit
Human Obestatin ELISA Kit
Chicken follicle-stimulating hormone,FSH ELISA Kit
TAF2 ELISA Kit
ALT ELISA Kit
Human oxytocin，OT ELISA Kit
Human Vascuoar endothelial cell growth factor receptor 1,VEGFR-1/Flt1 ELISA Kit
AGT ELISA Kit
Medium 11（Neomycin Assay Agar）
Chopped Meat Carbohydrate Broth（CMC）
Isobutyl Alcohol
Glycine Ethyl Ester Hydrochloride
5-Chloro-2-Hydroxyacetophenone
2-Fluoro-N-(2-fluoro-4-iodophenyl)benzaMide, 97%
o-Vinyltoluene
Reactive Black 5
Tween(R) 80
5'-Fluoro-2'-hydroxyacetophenone
5-AMINO-2-METHOXYBENZOTRIFLUORIDE
3-Nitrobenzyl bromide
Phosphorus(V) chloride, 99.998% (Metals basis)
(1R,2R,3S,5R)-(-)-2,3-Pinanediol, 99%
3-Hydroxy-4'-Methoxyflavone, 97%
1-BroMo-2-butyne
1-AMino-3,6,9,12,15,18-hexaoxahenicosan-21-oic Acid
1,1,1-TRIFLUORO-2-PROPANOL
2-Chloro-6-fluorobenzo[d]thiazole
2,2',5,5'-Tetrachlorobenzidine
4-BroMo-N-[2-(TBDMSO)ethyl]benzenesulfonaMide
3-Fluoro-5-(2,2,2-trifluoroethoxy)benzeneboronic acid 98%
Tetramethoxymethane
ethyl 4-amino-1,2,5-oxadiazole-3-carboxylate(SALTDATA: FREE)
Methyl 2-Methoxyphenylacetate
