HCO细胞株：人颅骨成骨细胞系促销多 货号：GD-C8519607
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-Chloro-6,7-diMethoxyquinazoline
FMoc-Glycinol
(2,3-Difluorophenyl)thiourea
Cobalt tetraMethoxyphenylporphyrin
DiMethylaMinoethyl acrylate
2-AMino-5-guanidinovaleric acid Monohydrochloride
Ethyl cyanoformate, 99%
Tetrabutylammonium Difluorotriphenylstannate
2,3,7-Trimethylindole, 99%
2-Fluoro-5-methyl-3-nitropyridine
6-(4-Aminophenyl)-4,5-dihydro-5-methyl-3(2H)-pyridazinone
Methyl vinyl sulfone
4-Mercaptophenylboronic acid
Homovanillyl alcohol
Metanil Yellow
6,8-Dichloro-2-oxo-2H-chroMene-3-carboxylic acid
4,4'-Ethylenedianiline
3-Methoxypyridine-2-carbaldehyde
tert-Butyl N-[4-(aMinoMethyl)phenyl]carbaMate, 97%
BENZIDINE
Dodecylbenzenesulfonyl Azide (soft type) (Mixture)
2,3-dimethyl-2-cyclopenten-1-one
1,1-dimethoxydecane
Phenyldimethylethoxysilane
2,3-dimethoxy-5-methyl-1,4-benzoquinone
2-Nitro-p-cresol
2,3,4,5,6-PENTABROMOETHYLBENZENE
1-Butyl-3-MethyliMidazoliuM Nitrate
3-Cyano-2-fluorobenzeneboronic acid pinacol ester, 96%
3-(4-Chlorobenzoyl)propionic acid, 98%
2-BroMo-4'-nitroacetophenone, 95%
[6]-Gingerol
trans-1,3-Dichloropropene
N3-BENZYL-2-CHLORONICOTINAMIDE
N-(2-CYANOETHYL)-N-(2-HYDROXYETHYL)-M-TOLUIDINE
(Perfluoroisopropyl)phenyliodoniuM TrifluoroMethanesulfonate , 97%
Trans-2-(3-Methoxyphenyl)vinylboronic acid pinacol ester
2-bromo-6-t-butylbenzothiazole
Mesitylcopper(I), min. 97%
Anthraquinone ,98%
3,4-Dihydroxybenzyl Alcohol
CADMIUM HYDROXIDE
1,3-Dimethyl-5-phenylbarbituric acid
PTH-GLYCINE
(5-BroMo-3-benzo[b]thienyl)Methanol
1-Dodecyne
2,2-DiMethylbutyryl Chloride
Methyl 4-broMo-1-naphthoate
1-Hydroxy-2,2,4,5,5-pentaMethyl-3-iMidazoline-3-oxide
TBHQ, FCC
4,4'-(Hexafluoroisopropylidene)diphthalic Anhydride
2-methyltricyclo[3.3.1.1~3,7~]decane
Mordant yellow 8 (C.I. 18821)
Tri(Hydroxymethyl) Amino Methane Hydrochloride
P-Hydroxybenzene propanoic acid
(R)-(-)-6,6'-Dibromo-1,1'-bi-2-naphthol
Diethyl isopropylideneMalonate, 97%
Dabsyl-L-Methionine
5-BroMo-2-Methoxyphenol
N-Methoxy-N-Methyl thiazole-5-carboxaMide
Nitrotriacid
Methyl 1-methyl-2-sulfanyl-1H-imidazole-5-carboxylate
Tributyl(cyanomethyl)phosphonium Chloride
N-Ethoxycarbonyl-3-nitro-p-toluidine
2',6'-DIFLUOROPROPIOPHENONE
4-(N-BOC-AMINOMETHYL)ANILINE
BROMOCHLORODIFLUOROMETHANE
3',4',5'-TRIFLUOROACETOPHENONE
2,1,3-Benzothiadiazole-5-sulfonyl chloride
3-(DicyanoMethylidene)indan-1-one
5-BroMo-2-Methylthiazole
Quinaldine red, pure
Iodine Monochloride, Reagent, ACS
(S)-(+)-DBD-Py-NCS [=(S)-(+)-4-(N,N-DiMethylaMinosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling]
dimethyl fluoromalonate
Flunisolide Hemihydrate
2,3,4-Trimethoxybenzoic acid
2,4,5-Trichlorophenol
CORT ELISA Kit
CD30 ELISA Kit
PANDER ELISA Kit
Human apoptosis protease activating factor-1,Apaf-1 ELISA Kit
PCX ELISA Kit
IL-31 ELISA Kit
PROG ELISA Kit
Human Factor-related Apoptosis ligand,FASL ELISA Kit
KLH ELISA Kit
5-LO/LOX ELISA Kit
SBG Sulfa Enrichment
Buffered Peptone Water
Ethyl phosphorodichloridite, 98%
trans-Anethole, 98+%, stab.
Ethyl 4-acetylbutyrate, 97%
Ethyl 2-AMino-4-Thiazolecarboxylate
2,3-dimethyl-2-cyclopenten-1-one
cis-3-Hexenyl butyrate
1-Benzyl-2-methyl-1H-imidazole
3-Hydroxy-4-nitrobenzoic acid
2,1,3-Benzothiadiazole
3-Bromobenzoic acid
Allylboronic Acid Pinacol Ester
2,5-DibroMo-3-Methylthiophene, 98%
1-BenzylhoMo, 98%
TripropylaMine
Orexin B rat, Mouse
1,3-Dioxolane
N-SucciniMidyl 4,4,5,5,6,6,7,7,8,8,9,9,9-tridecafluorononanoate
5-Methylisoxazole
Vinyl crotonate
5-Bromo-1-benzofuran-2-carboxylic acid, 97%
Guaiacol Carbonate
Dimethylaminotrimethyltin
N-Boc-hexahydro-1H-azepin-4-ol
