MHCC97-L(MHCC97L)细胞株：人低转移肝癌细胞系促销多 货号：GD-C8518846
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2,4-Dichlorobenzyl chloride, 98%
Triethoxysilane
Methyl Heptafluoroisobutyrate , 98%
2-[1-(MercaptoMethyl)cyclopropyl]acetic acid
Ethyl cellulose
1-BroMo-4,5-diMethoxy-2-nitrobenzene
5-Methyl-2-(trifluoroMethyl)benzonitrile, 97%
Pyrazine-2-carboxaldehyde
4-(4-chloro-6-ethylpyrimidin-2-yl)morpholine(SALTDATA: FREE)
3-Methyl-3-heptanol
1,2-Dimethylnaphthalene
3-(4-METHOXYPHENYL)-1H-PYRAZOLE-4-CARBALDEHYDE
ZINC TUNGSTATE
4-Chloro-2-(trifluoromethyl)benzoic acid
DL-tert-Leucine
2-Methylsulphonylaniline hydrochloride
(S)-3,3'-DibroMo-2,2'-diMethoxy-1,1'-binaphthyl
N-Butyl 4-BroMo-3-MethoxybenzaMide
Tetrakis(diMethylaMino)zirconiuM
Nile Blue A
(S)-2-(AMinoMethyl)-1-(tert-butoxycarbonyl)pyrrolidine
Glutaronitrle
4-AMINO-N-(6-CHLORO-5-METHOXY-4-PYRIMIDINYL) BENZENESULFONAMIDE
trans-2-penten-1-al
Dimethyl phthalate
METHANOL-D4
HECTORITE
5-Methoxyindole
3-FluorobenzenesulfonaMide, 97%
2-AMino-5-fluorophenol, 97%
2-AMino-4-tert-butylphenol
AMMoniuM nitrate
PYRAZINE-2-CARBOTHIOAMIDE
SodiuM 2-biphenylate
3-Fluoro-4-aMinobenzonitrile
4-(4-Methoxyphenyl)butyric acid
Ethyl 3-oxo-3-(2,4,5-trifluorophenyl)propanoate
6-(Dibutylamino)-1,3,5-triazine-2,4-dithiol
4-Methoxy-2-(trifluoromethyl)benzonitrile
Diacetato[(S)-(-)-2,2'-bis(diphenylphosphino)-5,5',6,6',7,7',8,8'-octahydro-1,1'-binaphthyl]ruthenium(II) Ru(OAc)2[(S)-H8-binap]
3-Amino-4-chlorophenol
TRILINOLEIN
1-(2-AMINOETHYL)-4-BENZYL
carbonic acid: cyclohexanamine
Ethylxanthic acid potassiuM salt
4-Chloro-3-nitrocinnaMic acid
Methyl Tricosafluorododecanoate
N-(2-Fluoro-4-iodophenyl)acetaMide
3,5,7-TriMethyladaMantane-1-carboxylic acid, 97%
Toradol
Acid Red 27
1-(4-Fluorophenyl)-1-Methyl-Ethylamine
fibrinopeptide B human
3-[[4-[(2,6-dichloro-4-nitrophenyl)azo]phenyl]ethylamino]propiononitrile
2-Isopropylthioxanthone
Isopropyl isothiocyanate
GerMane
DisodiuM 1,3-Benzenedisulfonate , 55%
4,4',4'',4'''-TETRA-TERT-BUTYLPHTHALOCYANINE COPPER
Isopropyltriphenylphosphonium Iodide
3-Fluoro-4-[(2-hydroxyethyl)carbamoyl]benzeneboronic acid 98%
3-Oxo-cyclopentanecarboxylic acid ethyl ester
Scandium(III) nitrate pentahydrate (99.9%-Sc) (REO)
3-Nitrocinnamic acid
5-[2-Cyclopropyl-1-(2-fluorophenyl)-2-oxoethyl]-5,6,7,7a-tetrahydrothieno[3,2-c]pyridin-2(4H)-one
1-BROMO-2,4,6-TRICHLOROBENZENE
4-Trifluoromethylphenol
6-Methoxy-1-indanone
1,2-Cyclododecanediol (cis- and trans- Mixture)
3-(Piperidino)phenylboronic acid
HexaMethylphosphorous triaMide, 97%
N-[(1,3-DiMethyl-1H-pyrazol-5-yl)Methyl]-N-MethylaMine, 97%
4-(Methylthio)benzyl Alcohol
MethyltrioxorheniuM(VII)
3-methoxypropionic acid
2-Vinylpyridine
O-(1,2-dihydro-2-oxo-1-pyridyl)-bis(te. methylene)uron. pf6
HECAMEG
Porcine VEGFR1/Flt1 (Vascuoar Endothelial Growth Factor Receptor 1) ELISA Kit
NT-ProBNP ELISA Kit
Human adiponectin，ADP ELISA Kit
Human CAPS ELISA Kit
CKMT1A ELISA Kit
Rat GATA binding protein 4,GATA4 ELISA Kit
Monkey Insulin,INS ELISA Kit
Human FGF17 ELISA Kit
TARC/CCL17 ELISA Kit
GPBB ELISA Kit
Pseudomonas Cepacia Agar Base
Bordet-Gengou Agar Medium Base
IndoMethacin, 98%
COPAIBA OIL
5-Phenyl-1,3,4-thiadiazole-2-thiol, 96%
2-Methyl-3-Nitropyridine
Glutaronitrle
Poly(vinyl formal)
D-Lysine Hydrochloride
kanamycin sulfate mixture of components A
1,2-Dibromobenzene
THIAZOLIDINE-2-CARBOXYLIC ACID
D-GlucosaMine hydrochloride
2-(1-Naphthyl)pyrrolidine, 95%
Methyl 4-azaindole-2-carboxylate, 95%
4-Methoxybenzenesulfonyl chloride, 98+%
(S)-Ru(OAc)2(T-BINAP)
2-AcetaMido-2-deoxy-D-glucose
6-AMino-2-Mercaptobenzothiazole , 95%
Tetrahydro-2-Methyl-3-thioxo-1,2,4-triazine-5,6-dione
N-DODECYL CYANIDE
BIS(PENTAMETHYLCYCLOPENTADIENYL)IRON
4-Fluoro-2-(trifluoromethyl)cinnamic acid
2-(Trifluoromethyl)benzoic acid hydrazide 95%
1,4-Bis(bromomethyl)naphthalene (contains ca. 23% isomer)
