HT-29细胞株：人结直肠腺癌细胞系技术过硬 货号：GD-C8418851
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2,6-Difluoro-4-iodophenol, 99%
MethylMercury chloride
2,6-Dichloro-5-fluoro-3-pyridinecarbonitrile
CyclohexylMethanethiol acetate
4-CHLORO-2-(TRIFLUOROMETHYL)PHENYL ISOCYANATE
Bis(tetraethylaMMoniuM) bis(2-thioxo-1,3-dithiole-4,5-dithiolato)zincate, 97%
1,2,3,6,7,8-HEXAHYDROPYRENE
3-Hydroxypyridine
Pentamethyliodobenzene
4-[(Methylcarbamoyl)amino]benzeneboronic acid, pinacol ester 95%
(Phenyldimethylsilyl)methyl methacrylate(Methacryloxymethyl phenyldimethylsilane)
2,5-Dimethyl-2,5-hexanediol
1-BROMO-3-ISOPROPOXYBENZENE
IODINE MONOBROMIDE
Methyl 6-bromo-2-naphthoate
DansylaMide
2,2-DiMethylhexanoic Acid
t-Butyl 4-broMo-1-naphthoate
TetraMethylaMMoniuM broMide, 98%
Nisin
4-BroMobenzyl Cyanide
ammonium oleate
2-(1-Naphthoyl)-benzoic acid
dec-1-yne
A-naphthyl red hydrochloride
1-(4-Bromophenyl)octane
TRIETHANOLAMINE LAURYL SULFATE
Isopropyl BroMide
LithiuM hexafluorophosphate, 98%
2-(4-Methyl-5-thiazolyl)ethyl acetate, 98+%
4-Hydroxy-1-indanone
p-Fluoro benzaldehyde
2-OCTYNOIC ACID
SODIUM 1-UNDECANESULFONATE
5-AMinooxindole
2-Hexyne, 98+%
5-Fluoro-2-(trifluoromethyl)benzaldehyde 97%
5-(3,4-Dichloro-phenyl)-1 H -pyrazole-3-carboxylic acid
[4-(5-methyl-1H-tetrazol-1-yl)phenyl]acetic acid(SALTDATA: FREE)
2-Amino-3-bromobenzotrifluoride 98%
1,4-Butane sultone
BIS(4-NITROPHENYL) SULFIDE
Oxazole
HOLO-TRANSFERRIN
D-Xylopyranose
2-AMino-6-Methoxypurine
5-BroMo-2-hydroxy-4-trifluoroMethylpyridine
3-Methyl-2-oxobutanoic acid, sodiuM salt, 98+%
3,5-Dichloro-2-hydroxybenzenesulfonyl chloride, 90-95%
CELLULASE
4-Methyl-2-phenyliMidazole
1-Bromopropane
(S)-(+)-N,N-Dimethyl-3-Napthtoxy-(2-Thiophene) Propylamine Oxalate
2-amino-3-acetylpyridine
2-Chloro-3,4-diaminopyridine
(3-CHLORO-PHENYL)-METHANESULFONYL CHLORIDE
3-Chloroperoxybenzoic acid
2-AcetaMido-5-fluorophenylboronic acid, pinacol ester
Tetrakis(triphenylphosphine)platinuM
4-Methyl-4-cyclohexene-1,2-dicarboxylic anhydride
Imidazo[1,2-a]pyridin-6-ylmethanol, 97%
Nimodipine
Poly(hexadecyl methacrylate), 28% solution in toluene, average M.W. 190.000
Methyl 4-(butyrylamino)-3-methyl-5-nitrobenzoate
Hexabromocyclododecane
1-PHENYLISATIN
Guanidine nitrate
Methyl chloroacetate
1,2-DibroMo-3-chloropropane
2-Methoxy-4-Methylpyridine
4,5-DiMethyl-4H-1,2,4-triazole-3-thiol
Bis(1,5-cyclooctadiene)iridiuM(I) tetrafluoroborate, 98%
POTASSIUM HYDROGEN D-TARTRATE
9-AnthrylMethyl Acrylate
Methyl 4-(aminomethyl)benzoate
O-6-ethoxy-2-ethylpyrimidin-4-yl O,O-dimethylphosphorothioate
diboron calcium tetraoxide
Praseodymium acetate
PROCR ELISA Kit
Mouse carcinoembryonic antign,CEA ELISA Kit
Adiponectin ELISA Kit
CNX ELISA Kit
MT ELISA Kit
GATA5 ELISA Kit
P ELISA Kit
Human FGF21 ELISA Kit
Human TSLP ELISA Kit
HBP ELISA Kit
Chapman Agar Medium
Blood Agar Base with Potassium Tellurite
Indole-7-carboxylic acid, 97%
Alverine citrate
5-AMino-1H-indazole, 98%
2-Methyl-3-Thiophenecarboxylic Acid, Ethyl Ester
ammonium oleate
Resins, terpene
beta-D-Allose
2-(4-thiazolyl)benzimidazole
ETHYL 2-CYANOACETOACETATE
1,3-DIAMINO-2-PROPANOL-N,N,N',N'-TETRAACETIC ACID
Boc-Osu
3-AMino-4-chloro-1H-indazole, 97%
Polyethylene, chlorinated
Ethylene glycol diMethyl ether
Diethyl (3-Chlorobenzyl)phosphonate
Hydrogen broMide
4-(TrifluoroMethylthio)benzaMide
CHROMIUM(III) NITRATE NONAHYDRATE
Benzoin resin absolute
Oxamic hydrazide, 99%
2-Methyl-5-nitroindole, 95%
2,3-Difluoro-6-nitroaniline
Isoquinoline-8-carbonitrile
