AKR细胞株：小鼠食管癌细胞系技术过硬 货号：GD-C8418762
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Hexanophenone, 98%
2-Fluorotoluene
D-Leucinol
2-(N-Boc)-5-(tributylstannyl)thiazole
5-CHLORO-3-METHYL-1-PHENYL-1H-PYRAZOLE-4-CARBONITRILE
2-FLUORO-5-METHOXYBENZALDEHYDE
2-AMino-3-chloro-5-trifluoroMethylpyridine
Diethyl dimethylmalonate, 97%
n-Octanohydrazide
Phthalimidoacetone
5-Amino-1H-tetrazole
(1S,3R)-3-Aminocyclopentanecarboxylic acid
ETHYL N-PROPYL ETHER
2-AMINO-1-PHENYLETHANOL
Heptanoyl chloride
RhodaMine 123
Diethoxy(3-glycidyloxypropyl)Methylsilane
6-BroMotetrazolo[1,5-a]pyridine
tert-Butyl acrylate, 99%, stabilized
Mitoxantrone Hydrochloride
DBD-ED [=4-(N,N-DiMethylaMinosulfonyl)-7-(2-aMinoethylaMino)-2,1,3-benzoxadiazole] [for HPLC Labeling]
1-iodo-2-methylpropane
3-(Hydroxymethyl)-piperidine
6-(Trifluoromethyl)uracil
Phthalazone
S-(4-CHLOROBENZYL)ISOTHIOURONIUM CHLORIDE
TOLYLFLUANID
Ethylbenzene, 99%
AluMinuM sec-butoxide, 97%
N-Boc-O-benzyl-D-threonine, 98%
p-Chlorotoluene Standard
1,2,5,6,9,10-HexabroMocyclododecane
3-BENZYL-5-(2-HYDROXYETHYL)-4-METHYLTHIAZOLIUM CHLORIDE
STRONTIUM SULFIDE
3-TrifluoroMethoxyphenylboronic acid
O,O′-Diethyl Methylphosphonothioate
2-Fluoro-3-methylbenzoic acid 98%
3,5-Diacetoxybenzoic acid
9-Anthracenecarboxylic acid ,99%
3-Carboxy-4-chlorobenzeneboronic acid
BIS[3-(TRIMETHOXYSILYL)PROPYL]ETHYLENE DIAMINE
VISNAGIN
5-BROMO-2-FUROIC ACID HYDRAZIDE
Ketoprofen Related CoMpound C;2-(3-carboxyphenyl)propionic acid
4-Methoxy-2-nitroaniline
(S)-(-)-DBD-Pro-COCl [=(S)-(-)-4-(N,N-DiMethylaMinosulfonyl)-7-(2-chloroforMylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. DeterMination]
1-BOC-6-cyanoindole-3-boronic acid, pinacol ester
4-Nitrotoluene, 99%
3,4,5-Trifluorobenzaldehyde, 98%
2-BUTENE-1,4-DIOL
Riboflavin
5,8-dihydroxy-1,4-naphthoquinone
4-Bromo-2-ethyliodobenzene
3-Acetamidopyrrolidine
N(alpha)-boc-N(delta)-(9-xanthenyl)-L-glutamine
9-Fluorenone
1-BroMopyrene
2,5-Dichloropyridine-4-carboxylic acid
PHTHALOCYANINE LEAD
1H-indole-4-carboxylic acid(SALTDATA: FREE)
5-Bromo-3-(chloromethyl)-1-benzothiophene , 97%
5-chloro-2-isobutoxybenzaldehyde(SALTDATA: FREE)
5-Amino-8-hydroxyquinoline Dihydrochloride
(R)-Glycidyl butyrate
OXAMIC ACID
Trimethylphenylammonium chloride
2',4'-Dichlorovalerophenone
Isopropyl trifluoroacetate
1,10-Bis(4-carboxyphenoxy)decane
Ethyl 5-MethyliMidazo[1,2-a]pyridine-2-carboxylate
alpha-Methylstyrene, 99%, stabilized
2,3-Dichlorobenzoic acid, 98%
2-Ethylhexyl 4-Hydroxybenzoate
Triphenylethylene
4-Methoxybiphenyl
2-chloro-1,4-difluoro-3-methoxybenzene
2'-Fluoro-6'-hydroxyacetophenone
Methyl 2-methylnicotinate
PAFR ELISA Kit
IL19 ELISA Kit
Human NE/ELA2 (Elastase 2, Neutrophil) ELISA Kit
Human GAL ELISA Kit
Chicken tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 ELISA Kit
CXCR3 ELISA Kit
Monkey TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit
Human Enterovirus ELISA Kit
Human STAT4 ELISA Kit
Eosinophil-Rnase A2 ELISA Kit
Drosophila Medium
Lowenstein-Jensen Medium Base, Modified
Oil Red O
PODOPHYLLOTOXIN
Floxuridine
AMyl-2-Methylbutyrate
1-iodo-2-methylpropane
silver chromate
6-Chloropyrazine-2-carbonitrile
Phenylbutazone Sodium Salt
4-Fluorobenzophenone
5-Chloroisatoic Anhydride
Benzyl 3-hydroxyphenylacetate, 98%
3,6-DiMethyl-3,6-octanediol, (^+) + Meso, 97%
TETRABUTYLAMMONIUM DIFLUOROTRIPHENYLSTANNATE
HoMosalate
3-MaleiMido-PROXYL
2-Nitrochlorobenzene
4-(Cbz-AMino)-2-fluorophenylboronic acid, pinacol ester
2,6-Dichloro-3-(trifluoromethyl)pyridine
Naphthol AS-BI phosphate ,95%
2,3-Dimethoxycinnamic acid
2-Fluoro-6-methyl-3-nitropyridine
