SEM细胞株：人前B淋巴白血病细胞系技术过硬 货号：GD-C8419507
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
为什么培养的细胞要及时传代？体外培养的细胞大多具有生长接触YZ的特性，也就是说当一个细胞被其他细胞包围的时候，它就会停止生长，及时传代后，细胞的生长得以继续。培养细胞生长减慢的原因在哪些?其解决办法有哪些？可能的原因有：更换了不同的培养液或血清；培养液中一些细胞生长必需成分如谷氨眈肢或生长因子等耗尽或缺乏或己被破坏；培养物中有少量细菌或真菌污染；试剂保存不当；比较新培养液与原培养液成分，比较新血清与旧血清等，找出其它可能的原因。解决办法：增加起始培养细胞浓度；让细胞逐渐适应新培养液；换入新鲜配制培养液；补加谷氨酷肢或生长因子等；用无抗生素培养液培养，如发现污染，去弃培养物，或用抗生素CJ；血清需保存在5℃到20℃; 培养液需在2-8℃避光保存;含血清完全培养液在2-8℃保存，并在2周内用完;分离培养物，检测支原体。冷冻管应如何解冻？取出冷冻管后，须立即放入37℃水槽中快速解冻，轻摇冷冻管使其在1-2分钟内大部分融化，特别注意，需残留一小块冰块，就可拿到超净工作台操作细胞，用75%酒精消毒冻存管，用新鲜培养基将细胞转移至培养瓶。另外冷冻管由液氮桶中取出解冻时，必须注意安全，预防冷冻管爆裂。
为什么培养的细胞要及时传代？体外培养的细胞大多具有生长接触YZ的特性，也就是说当一个细胞被其他细胞包围的时候，它就会停止生长，及时传代后，细胞的生长得以继续。培养细胞生长减慢的原因在哪些?其解决办法有哪些？可能的原因有：更换了不同的培养液或血清；培养液中一些细胞生长必需成分如谷氨眈肢或生长因子等耗尽或缺乏或己被破坏；培养物中有少量细菌或真菌污染；试剂保存不当；比较新培养液与原培养液成分，比较新血清与旧血清等，找出其它可能的原因。解决办法：增加起始培养细胞浓度；让细胞逐渐适应新培养液；换入新鲜配制培养液；补加谷氨酷肢或生长因子等；用无抗生素培养液培养，如发现污染，去弃培养物，或用抗生素CJ；血清需保存在5℃到20℃; 培养液需在2-8℃避光保存;含血清完全培养液在2-8℃保存，并在2周内用完;分离培养物，检测支原体。冷冻管应如何解冻？取出冷冻管后，须立即放入37℃水槽中快速解冻，轻摇冷冻管使其在1-2分钟内大部分融化，特别注意，需残留一小块冰块，就可拿到超净工作台操作细胞，用75%酒精消毒冻存管，用新鲜培养基将细胞转移至培养瓶。另外冷冻管由液氮桶中取出解冻时，必须注意安全，预防冷冻管爆裂。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
CeriuM(III) TrifluoroMethanesulfonate
4-BroMo-1,2-diMethylbenzene
4-PYRIDINEETHANETHIOL HYDROCHLORIDE
2,2-DIMETHOXYPROPIONIC ACID METHYL ESTER
4-(N,N-DIMETHYLAMINO)AZOBENZENE-4'-ISOTHIOCYANATE
1-ETHYL-2-METHYLCYCLOHEXANE
8-Methoxy-2,3,4,5-tetrahydro-benzo[c]azepin-1-one
N-Methyl glucamine antimonate
Dichloro{(S)-(-)-2,2'-bis[di(3,5-xylyl)phosphino]-1,1'-binaphthyl}[(2S)-(+)-1,1-bis(4-methoxyphenyl)-3-methyl-1,2-butanediamine]ruthenium(II) RuCl2[(S)-xylbinap][(S)-daipen]
Nalpha-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-lysine Hydrochloride
Ethylene Glycol Dibutyl Ether
TRANS-2-(4,4,5,5-TETRAMETHYL-1,3,2-DIOXABOROLAN-2-YL)STYRENE
4-(DIMETHYLAMINO)PHENYL ISOTHIOCYANATE
Quinagolide hydrochloride
Bis(diphenylphosphino)Methane
2-Fluorobenzenesulfonyl chloride
EthylenediaMinetetraacetic Acid DisodiuM Zinc Salt Hydrate
4-Fluoro-3-hydroxybenzaldehyde
1-BroMo-2-(difluoroMethyl)benzene, 97%
2,5-DIBROMOTOLUENE
4,5-Ethylenedithio-1,3-dithiole-2-thione
2-Bromo-5-fluoroaniline
Calcium
methyl 2-pyridylacetate
quinolin-7-ol
2-METHOXYBENZHYDRAZIDE
L-Threonine
BariuM TrifluoroMethanesulfonate
4-Chloro-2-fluorocinnaMic acid, 98%
4'-Hydroxy-3'-nitroacetophenone, 98%
2-(Boc-aMino)-5-(aMinoMethyl)pyridine
5-BroMo-2-Methylbenzotrifluoride
MagnesiuM fluoride
N-Carbobenzyloxy-L-alanine
2-CINNAMOYLTHIOPHENE
ZIRCONIUM(IV) ETHOXIDE
1-Pyrazin-2-ylmethanamine hydrochloride
Strontium chloride, anhydrous, (trace metal basis), 99.99%
9-Fluorenemethanol ,99%
4-Bromo-1,8-naphthalic Anhydride
6-Cyanoindole
1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE
CALCIUM SILICIDE
HEPARINASE
N-MethylbenzaMide
Tris(3-Methoxyphenyl)phosphine
1,3,5-Tris(4-aMinophenyl)benzene
2-Nonanone, 99%
N,2-DiMethyl-(1,3-thiazol-4-yl)MethylaMine, 97%
Dill Oil
4-Cyano-4'-heptylbiphenyl
1,3-Diphenyl-1,1,3,3-tetramethyldisiloxane
ethyl 2,4-difluorobenzoate
guanosine 5'-(disodium dihydrogen triphosphate)
iso-Decyl methacrylate
3-Aminopyrazole
Bicyclo[2.2.1]hept-2-en-2-ylboronic acid
2-BenzylaMinopyridine
5-HydroxyMethyl-2,2,5-triMethyl-1,3-dioxane
2-Methyl-1,3-dithiolane, 97%
Dimedone Dioxime [for Determination of Co]
(5-Trifluoromethylpyridin-3-yl)boronic acid, 95%
2-Bromo-1-(2,5-dichlorophenyl)ethanone
BIS(2-CHLOROISOPROPYL)ETHER
D-Lactose monohydrate
(Z)-2-(tert-Butoxycarbonylprop-2-oxyimino)-2-(2-tritylaminothiazol-4-yl)acetic acid
2-Methylbenzoxazole
Butyl Methacrylate
2-(2-BroMoethyl)-2-Methyl-1,3-dioxolane
DiMethyl 2-Methylterephthalate
AMMoniuM hydrogen difluoride, 95%, extra pure
4-Nitrophenyl forMate, 97%
1-Morpholino-1-cyclohexene
4-NitrobenzylaMine Hydrochloride
ethyl(methyl)amine
2,2'-(ethane-1,2-diylbis{[2-(aminooxy)-2-oxoethyl]imino})diacetic acid hydrate (non-preferred name)
FLUVASTATIN SODIUM
Pencycuron
Porcine CXCL16 (Chemokine C-X-C-Motif Ligand 16) ELISA Kit
DNMT3B ELISA Kit
Human hepatitis B virus X interacting protein,HBXIP ELISA Kit
Human SPLUNC2 ELISA Kit
Monkey Interleukin 6,IL-6 ELISA Kit
pro-IL1B ELISA Kit
α1M ELISA Kit
Human low molecular-weight protein/proteasome beta-type subunit,LMP7/PSMB9 ELISA Kit
Human HJV/HFE2 (Hemojuvelin/Hemochromatosis Type 2) ELISA Kit
DVD/DHVD 3 ELISA Kit
Chalmers Agar(Modified Chalmers Agar Base)
Tomato Juice Agar,Modified
Neryl Acetate
trans-4-(4-Pentylcyclohexyl)benzonitrile
4-Isopropylthiobenzeneboronic acid, 97%
2-Phenylthiazolidine, 97%
2-Bromo-5-fluoroaniline
Sodium persulfate
Cinnamaldehyde oxime
7-(B-hydroxypropyl)theophylline
2-Amino-6-chloro-4-pyrimidinol
ADIPIC ACID DIVINYL ESTER
Ethyl Methyl KetoxiMe
2H,3H-Perfluoropentane, tech. 90%
1,2-Dibenzoylethane, 98+%
tert-Butyl broMide
Cyclopentanepropionyl chloride
BIS(METHYLCYCLOPENTADIENYL)NIOBIUM DICHLORIDE
3,4,6-Tri-O-acetyl-2-deoxy-D-glucopyranose , 98%
FMoc-Gly-OPfp
2-BroMoMethyl-4-fluorophenylboronic acid
3',4'-Dichlorosalicylanilide
2,6-Bis(chloromethyl)pyridine
N-(4-Fluorophenyl)anthranilic acid
1,3-Diisopropylimidazolium Tetrafluoroborate
