FBHE细胞株：牛心内皮细胞系技术过硬 货号：GD-C8419435
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
为什么培养的细胞要及时传代？体外培养的细胞大多具有生长接触YZ的特性，也就是说当一个细胞被其他细胞包围的时候，它就会停止生长，及时传代后，细胞的生长得以继续。培养细胞生长减慢的原因在哪些?其解决办法有哪些？可能的原因有：更换了不同的培养液或血清；培养液中一些细胞生长必需成分如谷氨眈肢或生长因子等耗尽或缺乏或己被破坏；培养物中有少量细菌或真菌污染；试剂保存不当；比较新培养液与原培养液成分，比较新血清与旧血清等，找出其它可能的原因。解决办法：增加起始培养细胞浓度；让细胞逐渐适应新培养液；换入新鲜配制培养液；补加谷氨酷肢或生长因子等；用无抗生素培养液培养，如发现污染，去弃培养物，或用抗生素CJ；血清需保存在5℃到20℃; 培养液需在2-8℃避光保存;含血清完全培养液在2-8℃保存，并在2周内用完;分离培养物，检测支原体。冷冻管应如何解冻？取出冷冻管后，须立即放入37℃水槽中快速解冻，轻摇冷冻管使其在1-2分钟内大部分融化，特别注意，需残留一小块冰块，就可拿到超净工作台操作细胞，用75%酒精消毒冻存管，用新鲜培养基将细胞转移至培养瓶。另外冷冻管由液氮桶中取出解冻时，必须注意安全，预防冷冻管爆裂。
为什么培养的细胞要及时传代？体外培养的细胞大多具有生长接触YZ的特性，也就是说当一个细胞被其他细胞包围的时候，它就会停止生长，及时传代后，细胞的生长得以继续。培养细胞生长减慢的原因在哪些?其解决办法有哪些？可能的原因有：更换了不同的培养液或血清；培养液中一些细胞生长必需成分如谷氨眈肢或生长因子等耗尽或缺乏或己被破坏；培养物中有少量细菌或真菌污染；试剂保存不当；比较新培养液与原培养液成分，比较新血清与旧血清等，找出其它可能的原因。解决办法：增加起始培养细胞浓度；让细胞逐渐适应新培养液；换入新鲜配制培养液；补加谷氨酷肢或生长因子等；用无抗生素培养液培养，如发现污染，去弃培养物，或用抗生素CJ；血清需保存在5℃到20℃; 培养液需在2-8℃避光保存;含血清完全培养液在2-8℃保存，并在2周内用完;分离培养物，检测支原体。冷冻管应如何解冻？取出冷冻管后，须立即放入37℃水槽中快速解冻，轻摇冷冻管使其在1-2分钟内大部分融化，特别注意，需残留一小块冰块，就可拿到超净工作台操作细胞，用75%酒精消毒冻存管，用新鲜培养基将细胞转移至培养瓶。另外冷冻管由液氮桶中取出解冻时，必须注意安全，预防冷冻管爆裂。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
5-AMinolevulinic acid hydrochloride
5-(Ethylthio)-1H-tetrazole
PHLOROGLUCIDE
6-AMINOPHTHALIDE
5-MethylphenaziniuM Methosulfate
1-BOC-7-Methoxyindole
Methyl perfluoro-3,6-dioxadecanoate
4-(Methylsulfonyl)phenylhydrazine hydrochloride
N1-(2-Methyl-4-nitrophenyl)acetamide, 97%
Silver bromide (99.9%-Ag)
Ethyl trans-2-Decenoate
Z-CYS(BZL)-OH
3,6-Dihydro-3-methyl-N-nitro-2H-1,3,5-oxadiazin-4-amine
Palmitic acid ethyl ester
3,4,5-TriMethoxybenzylaMine
Cyclododecanol
2,6-Dichloro-7-deazapurine
3-(2-Acetylphenyl)benzoic acid
2-MesitylMagnesiuM broMide, 1M solution in THF, AcroSeal
Zinc Carbonate, USP
Quinaldine Red
Sodium Stearate
Cholesteryl chloroformate
Ethylhippurate
2,4-Dimethylthiazole
2-Chloro-4-nitrobenzoic acid
ALPHA-NAPHTHOFLAVONE
2,4,5-TrichloropyriMidine
2-Ethoxyacetyl chloride, 97%, stab. with 0.3% MagnesiuM oxide
2-Thiohydantoin, 99%
Pyrrolidine
Hexane-d14
N-TriMethylsilylbenzaldiMine
N-HEPTADECYLCYCLOHEXANE
1-PENTADECANESULFONIC ACID SODIUM SALT
4-Cyanobenzoic acid
2-Fluoroethan-1-ol
2-Phenyl
Cholesterol Laurate
Methyl 2-fluoro-2-methylpropanoate 90%
3-(TRIMETHYLSILYL)PHENYLBORONIC ACID
o-Carbomethoxybenzyl sulfonamide
3-METHYLISOQUINOLINE
4-Chlorobenzaldehyde
7-Ethoxy-4-Methyl-2H-chroMen-2-one
1-(1-AdaMantyl)ethylaMine hydrochloride
5-Isopropyl-3-(Methoxycarbonyl)-2H-cyclohepta[b]furan-2-one
5-BroMo-2-MethylaMino-3-nitropyridine
Methyl 4-iodobutyrate, 95%, stabilized
N,N-DIMETHYLOCTYLAMINE
3-Methyl-2-oxovaleric Acid
1,3-Benzenediamine, N-phenyl-
L-glutamic acid bis-allyl ester toluene 4-sulfonate
Cobalt(II) acetate tetrahydrate
4,4'-methanediylbis(2-methylphenol)
2-(TRIFLUOROMETHOXY)BENZAMIDE
4-AcetaMido-3-fluorophenylboronic acid, pinacol ester
2,5-Diethoxyaniline, 96%
4'-Fluoro-3'-nitroacetophenone
1,3-Thiazole-2-carbaldehyde , 95%
6-Methyl-5-nitro-1H-indazole
3-Bromo-N-(2-hydroxyethyl)benzenesulphonamide 97%
Diphenyl Isophthalate
Methomyl
Benzyl carbamate
4-METHYL-2,3,5,6-TETRAFLUOROBENZYL BROMIDE
ACID BLUE 1
Leuco Crystal Violet
1-Naphthaleneacetic Acid SodiuM Salt
Tolylene Diisocyanate (2,4- (=:)80%, 2,6- (=:)20%)
1-Methyl-4-piperidone, 98%
Triethyl orthobenzoate, 97%
(2S,3R,4S)-CCG
Meso-1,2-DibroMo-1,2-diphenylethane
4-fluoro-3-phenoxybenzaldehyde
Enalapril
Quadrosilan
dimethyl (1R,6R)-cyclohex-3-ene-1,6-dicarboxylate
BRCA1 ELISA Kit
GDI1 ELISA Kit
Human INH ELISA Kit
Human VS ELISA Kit
Monkey IgE (Immunoglobulin E) ELISA Kit
IL10Rα ELISA Kit
INH-B ELISA Kit
Human PTPRG ELISA Kit
Human PFKP ELISA Kit
Guinea pig transforming growth factor β1,TGF-β1 ELISA Kit
Violet-Red Bile Agar with Glucose and Lactose
Violet-Red Bile Agar with Glucose and Lactose
StrontiuM Acetate
Trans-Zeatin
4-n-Heptylbenzoic acid, 99+%
METHYL 2-PYRIDYLACETATE
Sodium Stearate
2-BENZYLOXY-1,3-PROPANEDIOL
Tungsten(VI) chloride
Liquiritigenin
1,1'-P-ANISYLIDENEBIS(2-NAPHTHOL)
3-Chloro-2,4,5,6-tetrafluoropyridine
N-Cbz-D-GlutaMic Acid Alpha-Benzyl Ester
5-BroMo-7-azaindole, 96%
6-Mercaptopurine Monohydrate, 98%
Luciferase froM Photinus pyralis (firefly)
N-Boc-D-tryptophanol
tert-Butyl 2-isocyanopropionate
TRIS(3-FLUOROPHENYL)PHOSPHINE
2,2,3,3,3-PENTAFLUOROPROPYL ACRYLATE
1-Iodo-2,4-diMethyl-6-nitrobenzene
N-A-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide[HPLC labelling reagent for e.e.determination)
(2-amino-3-chlorophenyl)amine(SALTDATA: FREE)
4-Formyl-5-(trifluoromethyl)-1-methyl-1H-pyrazole
2-Phenoxybenzyl Chloride
