BRL3A细胞株：大鼠肝细胞系技术过硬 货号：GD-C8419287
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Methyl p-toluate, 99%
N-Boc-piperidine-2-Methanol
(R)-Boc-3-(2-naphthyl)-β-Ala-OH
CalciuM DL-Glycerate Hydrate, 98%
2-SEC-BUTYLCYCLOHEXANONE
METHYL 2,3,4,6-TETRA-O-ACETYL-1-THIO-ALPHA-D-MANNOPYRANOSIDE
Diethyl 3-hydroxyglutarate
4-Nitrobenzenediazonium Tetrafluoroborate
Copper(II) hexafluoroacetylacetonate hydrate, 98%
4-Diazo-N,N-dimethylaniline Chloride Zinc Chloride Hydrate
(2,5-Dimethoxyphenyl)acetyl chloride, 99%
Sodium 3-sulfobenzoate
(S)-5-Hydroxymethyldihydrofuran-2-one
HEPES sodium salt
ChroMiuM(II) chloride
N-Boc-γ-aMinobutyric acid
9,10-Di(1-naphthyl)anthracene
Methyl 5-broMo-8-Methyl-4-oxo-1,4-dihydroquinoline-2-carboxylate
(R)-N-FMOC-3-Fluorophenylalanine, 95%, 98% ee
Tannic Acid, USP
Cyclopentadecane
2-Fluoroadenine
Benzidine Yellow G
COPPER CARBONATE
3-Pyridylacetic acid
D-alpha-Tocopheryl acetate
N-UNDECANOPHENONE
5-Nitroisophthalic Acid
1-Acetoxyacenaphthene, tech.
2'-Chloroacetanilide, 98+%
DMT-dG(ib) PhosphoraMidite
4-(TrifluoroMethyl)phenylhydrazine
BIS(4-HYDROXY-3,5-DIMETHYLPHENYL) SULFONE
N-TIGLOYLGLYCINE
Poly(acrylaMide)
ETHYLENEDIAMINE-N,N'-DIPROPIONIC ACID DIHYDROCHLORIDE
5-Hydroxyindan
5- p -Tolyl-1-vinyl-1 H -pyrrole-2-carboxylic acid
Sinigrin Hydrate
6-Chloro-2,3-difluoro-4-(trifluoromethyl)phenyl hydrazine 98%
2-Aminobenzylalcohol
5-ISOPROPYL-3-METHYLPHENOL
2,5-Norbornadiene
Trifluoro(methanol)boron
D-(-)-Tartaric Acid
2-Cyano-4-nitropyridine
7-Methoxy-4-MethylcouMarin
OsalMid
2,5-DibroMo-3,6-difluorobenzenesulfonyl chloride, 97%
Acid Blue 90
L-Tyrosine Benzyl Ester p-Toluenesulfonate
2-Mercapto-4(3H)-quinazolinone
4-Benzofurazancarboxaldehyde
vitamin K2
Tris(dimethylamino)phenylsilane
3-Methylpicolinonitrile
2,2'-Bipyridine-4,4'-dicarbaldehyde
DIMETHYL 5-NORBORNENE-2,3-DICARBOXYLATE
WaterMelon Ketone
(S)-beta-homoalanine hydrochloride
4-Aminopyridine-3-methanol ,97%
Tetrafluoroterephthalonitrile
1-(4-Methoxyphenyl) Dihydrochloride
3-(3-Indolyl)-2-oxopropanoic acid
2-Thiophenecarbonyl chloride
Gentamicin
Docusate sodium
5-(3-Hydroxyphenyl)-1H-pyrazole-3-carboxylic acid
1-Butyl-3-MethyliMidazoliuM Methanesulfonate
Ivabradine Hydrochloride
1-(3-BroMophenyl)cyclopropanaMine
5-(2-BroMophenyl)-1H-tetrazole, 99%
ALPHAZURINE A
S,S'-DiMethyl N-CyanodithioiMinocarbonate
2-Amino-6-mercaptopyrimidin-4(1H)-one
1-Isocyanoadamantane
Supracet brilliant red BD
methyl 4-oxotetrahydrothiophene-3-carboxylate
PLN ELISA Kit
Mouse Substance P,SP ELISA Kit
Human PPOX ELISA Kit
Human DPP4 ELISA Kit
17-KS ELISA Kit
Rat IFN-β (Interferon Beta) ELISA Kit
IgA ELISA Kit
ProteinC ELISA Kit
SERT ELISA Kit
F-TESTO ELISA Kit
Sugar Fermentation Medium Base
MIO Medium
Molybdenyl Acetylacetonate
Mercury(II) fluoride, 95%
5-(Methoxycarbonyl)Pyridine-2-Carboxylic Acid
Furylacrylic Acid
2-Fluoroadenine
4-Bromo-3-chlorophenol
Sodium silicate
11-octadecenoic acid
2-AMINO-6-HYDROXY-8-MERCAPTOPURINE
Xylene
Cupric Tartrate
SodiuM tetrachloroaurate(III) dihydrate, PreMion|r, 99.99% (Metals basis), Au 49-50%
2,4,5-TrihydroxypyriMidine, 98%
4-tert-Butylbenzenesulfonyl chloride
2,5-DiMethoxy-β-nitrostyrene
1-AMINO-2-METHYLNAPHTHALENE HYDROCHLORIDE
Methyl 2-tetrahydrofuroate
N-(4'-Iodobiphenyl-4-yl)-N-(3-Methylphenyl)aniline
[2.2]Paracyclophane
1-(2-Phenylethyl) 98%
Disodium 1,3-Benzenedisulfonate
Acetaldoxime
3-(1H-Benzoimidazol-2-yl)-4-chloro-phenylamine
