RBE细胞株：人肝胆管癌细胞系权威供应 货号：GD-C8319099
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
1-[3-(4-tert-Butylbenzoyl)propyl]-4-hydroxypiperidine, 97%
2-Chloropyridine-5-boronic acid pinacol ester
(R)-(-)-2-Pentanol
CUMENE
TETRACYCLOHEXYLTIN
1,5,9,13-TETRADECATETRAENE
4-Fluoroacetanilide
5-Bromo-3-indolyl-beta-D-galactopyranoside
4-(2-Bromoethoxy)benzeneboronic acid, pinacol ester 98%
Hexadecyl Sulfide
L(+)-Lysine monohydrate, 99%
2-ETHYLNAPHTHALENE
ISOFENPHOS
2-Chloro-5-fluorotoluene
4-Nitropyrazole
2-BroMo-3-furoic acid
DiMethyl 4-Hydroxyisophthalate
Methyl 2-aMino-4-(3-broMophenyl)pyriMidine-5-carboxylate
Trifluoroacetic acid, 99.5%, for biocheMistry
Ranitidine Hydrochloride, USP
Arecoline HydrobroMide
3-Hydroxy-4-methoxybenzaldehyde
Guanoxan sulfate
4-amino-2,3,5,6-tetrafluorobenzoic acid
3',3'',5',5''-tetraiodophenolphthalein
2-Fluorobenzylamine
PIRIMICARB-DESMETHYL
Phenyl Vinyl Sulfoxide
Cobalt(II) 2-Methoxyethoxide, 5% w/v in 2-Methoxyethanol, 99% (M.b.), packaged under Ar in resealable CheMSeal bottles
2,4-Dichlorobenzoic acid, 98%
2-Acetylbenzothiophene
Ipronidazole-OH
2,2-Diethoxy-N,N-diMethylethylaMine
PigMent Orange 13
TRIMETHYLSILYL DIETHYLPHOSPHONOACETATE
DIISOBUTYLAMINE HYDROCHLORIDE
3-(Dimethylamino)acrylonitrile
2-Octadecyl-1,4-dimethoxybenzene
4-(4-Methoxyphenyl)benzeneboronic acid
3,4-Dihydroxy-5-methoxybenzoic acid
4-Mercaptophenol
3-Fluoro-4-methoxyaniline
Amisulpride
Trisodium hexafluoroaluminate
(Acetyloxy)(5-nitro-2-furyl)Methyl acetate
Benzyl Methacrylate
Lignin (Dealkaline)
2-BroMo-6-(pyrrolidin-1-yl)pyridine-4-boronic acid, pinacol ester
4-(Morpholine-4-sulfonyl)-benzenesulfonyl chloride, Tech.
Copper(II) chloride dihydrate
1-Ethyl-3-MethyliMidazoliuM Trifluoro(trifluoroMethyl)borate
L-histidine dihydrochloride
1-Amino-cyclobutanecarboxylic acid
Tracid Brilliant Red B
hexane-2,5-diol
1-(2-NITROPHENYL)PIPERIDINE
3,5-Difluoro-4-Methoxyphenylacetic acid, 97%
1,3,5-CYCLOHEXANETRICARBOXYLIC ACID
5-CHLORO-4-METHYL-2-NITROANILINE
2-Hydroxy-4-phenylthiazole
5-(4-bromophenyl)-1,3,4-thiadiazol-2-amine(SALTDATA: FREE)
5-Pyridin-3-ylthiophene-2-carbaldehyde, 97%
Xylazine Hydrochloride
2,6-BIS(2,2,2-TRIFLUOROETHOXY)-3-BROMOBENZONITRILE
2,6-Dimethoxypyrazine
KETANSERIN TARTRATE
Pentaerythritol tetrakis(3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate)
2-BroMo-5-(trifluoroMethyl)-1,3,4-thiadiazole
1-Acenaphthenol
1-Pyridin-4-yl-ethylaMine
3-Iodopyridine, 99%
N-Methylbenzothiazole-2-thione, 99%
BETA-ESTRADIOL 17-ACETATE
2,4-Dihydroxybenzaldehyde
4-Chlorotoluene
Paricalcitol
Benzoic acid, 2-(1-methylethoxy)-
2,5-Dimethylterephthalonitrile
VE ELISA Kit
αHSG ELISA Kit
Human PS-2 ELISA Kit
Human CA211 ELISA Kit
IC ELISA Kit
THP ELISA Kit
C3 ELISA Kit
Human PIH ELISA Kit
Human sVCAM-1/CD106 (soluble Vascuolar Cell Adhesion Molecule 1) ELISA Kit
VEGF.C ELISA Kit
Murashige & Skoog Macronutrients and Micronutrient
Glfu Anaerobic Medium Broth
AluMinuM MagnesiuM isopropoxide, 99.95+% (Metals basis)
MagnesiuM 2-aMinoacetate
5-Chloro-2-hydrazino-3-iodopyridine, 95%
2-Fluorobenzylzinc chloride, 0.5M in THF, packaged under Argon in resealable CheMSeal^t bottles
3-Hydroxy-4-methoxybenzaldehyde
Reactive Violet 8
Chlorinated rubber
1-fluoro-2,3-dimethoxybenzene
2,4-HEXADIYNE
4,4'-Difluorodiphenylmethane
SodiuM Dihydrogen Citrate
5,6-DiMethoxy-1-indanone, 97%
3-Fluoro-4-iodoaniline, 98%
2,6-DibroMotoluene
2-(TrifluoroMethoxy)benzyl chloride
2-Cyanopyridine-4-boronic acid pinacol ester
2-(3-N,N-DiMethylaMino-propoxy)pyridine-5-boronic acid, pinacol ester
1-(2-Methoxyethyl)
2-ETHYL-4,4-DIMETHYL-2-OXAZOLINE
2-Chloro-4-methyl-5-nitropyridine
2,2,4-Trimethyl-1,3-pentanediol diisobutyrate, 98%
2-Methoxypyridine-5-boronic acid hydrochloride 96%
4-[(2-hydroxyethyl)(methyl)amino]benzoic acid(SALTDATA: FREE)
