H22细胞株：小鼠肝癌细胞系权威供应 货号：GD-C8318699
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-(4-Fluorophenyl)-1-isopropylindole, 98%
Stainless Steel powder
2,3-Dihydro-5-furylboronic acid
4-Chloro-8-Methylquinoline
n-Butyl vinyl ether
4-(4-Methylpiperazino)aniline
Ethyl 4-chloro-2-Methylthio-5-pyriMidinecarboxylate
Trimethylphosphate, min. 97%
4-Chlorophenoxyacetyl chloride, tech., 90%
Cyclopropyl(phenyl)methanamine
1-(Pyridin-4-ylmethyl)-1H-pyrazole-4-boronic acid,pinacol ester
3-(3-PYRIDYL)BENZALDEHYDE
1H-Benzimidazole-5-carboxylic acid
N,N,N',N'-Tetraphenylbenzidine
3-Nitro-2-pyridinol
4-Iodobenzoic acid
2,4-Diphenyl-4-Methyl-1-pentene
5-BroMo-2-(1H-pyrazol-1-yl)pyridine
StrontiuM chloride, 99.99%, (trace Metal basis), anhydrous
Menadione, Powder, USP
2-(EthylMethylaMino)pyridine
Chlorfenvinphos
Methyltaurinesodium salt
2-ethoxyethyl chloroformate
Maleic acid
2-Bromo-4-(trifluoromethyl)pyridine
1,5-DICHLOROANTHRAQUINONE
Methyl Indole-5-Carboxylate
Isopropylidene Malonate, 98%
Pyruvic aldehyde diMethyl acetal, 97+%
DBE-5 dibasic ester
4-Fluoro-3-(trifluoroMethyl)phenol
2-BroMo-6-chloro-4-nitroanisole
tert-Butyl acetate
L-GLUCOSE
1-ETHYL-3-METHYLIMIDAZOLIUM DICYANAMIDE
L-Leucine ,99%
4-(1,3-Dithiolan-2-yl)benzene-1-carbothioamide, 97%
N-Benzhydryl-N-methylamine
[1-(N-Boc-aminomethyl)-cyclohexyl]-acetic acid
2-CHLOROCINNAMOYL CHLORIDE
Dibenzofuran-3-sulfonyl chloride
1,2,3-TRIPHENYLGUANIDINE
SULFANITRAN
3-Chloro-4-fluorobenzoic acid
TAPS sodiuM salt
p-Tolylsulfur Pentafluoride
2-Thioxotetrahydro-1,3-oxazole, 98%
7-Hydroxy-4-(trifluoroMethyl)-2H-chroMen-2-one, 97%
3,5-DINITROANILINE
SodiuM 1,2-Naphthoquinone-2-diazido-4-sulfonate
5,7-Dichloro-8-quinaldinol
Ethanone, 2-bromo-1-(2-iodophenyl)-
Benzothiazole-2-acetonitrile
5-hydroxy-4-methoxy-2-nitrobenzoic acid
(R)-(-)-2-AMINOHEPTANE
5-Chloro-1-MethyliMidazole, 98%
2,4,5-TriMethylbenzonitrile
TetrabroMo-o-sulfobenzoic Anhydride , 98%
5'-INOSINIC ACID BARIUM SALT
(4-Fluorophenyl)thiourea 98%
2,4-Dibromo-2,4-dimethyl-3-pentanone, 98%
4-Methylumbelliferylphosphate disodium salt trihydrate, 99%
Allyl 2,3,4,6-tetra-O-acetyl-β-D-glucopyranoside ,98%
Diglycolic acid
2,6-Dimethoxypyridine
Rubidium nitrate
4'-Ethoxyacetophenone
1-(3,5-Dichlorophenyl)-2-hydroxyethylaMine
Dithizone Silver CoMplex
4-BroMo-5-(4-Methoxyphenyl)pyrazole
PotassiuM chlorate, 99+%, ACS reagent
FuMonisin B2
3-Chloropivaloyl Chloride
5-Methyl-1H-benzotriazole
S-Benzyl-L-cysteine
2-Chloroacetophenone
2-Butanone
NO ELISA Kit
IL22 ELISA Kit
Human Tumor necrosis factor β，TNF-β ELISA Kit
TGL ELISA Kit
GLβ ELISA Kit
CD320 ELISA Kit
Monkey IL-10 (Interleukin-10) ELISA Kit
Human heat-labile enterotoxin B subunit,LTB ELISA Kit
Human ACA-IgM ELISA Kit
NT-4 ELISA Kit
Rappaport-Vassiliadis R10 broth
Tryptone Glucose Yeast Broth Medium
Triethyl orthopropionate, 98%
SodiuM perborate tetrahydrate, 97%
5-Hexen-1-ol, 98%
2-Methylnonane, 98+%
Chlorfenvinphos
Butanoic acid, 3-oxo-, 2-methylpropyl ester
Solvent blue 4 (C.I. 44045:1)
pteroic acid
DIBROMOBAPTA
(1'S, 3S)-1-(1'-PHENYLETHYL)-5-OXO-3-PYRROLIDINE CARBOXYLIC ACID
Delta-Nonalactone
Allyl phenyl ether, 99%
N-Boc-DL-proline, 98%
1-Butyl-1-MethylpyrrolidiniuM Chloride
PSS-Octavinyl substituted
3-Carboxy-4-Methylphenylboronic acid
6-BroMoquinoline, HCl
2-NAPHTHYL LAURATE
CHLOROMETHYL BUTYRATE
Dibutyltin diacetate, pract.
Diethyl phosphonoacetaldehyde diethyl acetal, tech., 90%
Cadmium standard solution, 1 mg/ml Cd in 0.5N HNO3, for AAS
Benzophenone-2,4'-dicarboxylic Acid Monohydrate
