PT-67细胞株：小鼠逆转录病毒包装细胞系体外培养 货号：GD-C8219720
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
养了几年细胞，说一点自己培养细胞方面的看法：1)培养细胞前，可以看看细胞特点说明书，包括细胞正常生长的状态图、传代、培养基的类型等；2)不同细胞生长周期不同，有的生长很快，比如说MDA-MB-231，传代的时候取离心悬液的十分之一就已经足够了，有的又是特别慢，等的人心焦，BT474就是，传代是一分二，复苏起始的时候两周多才能长满，所以就要在培养细胞的过程中多多摸索了；3)对于娇弱的细胞，就得细心呵护了，胰酶消化不能过久，吹得时候要轻柔，离心时候也要注意转速和时间，每天都要去看一下细胞，肉眼观察培养基状况、显微镜下观察细胞生长状况；4)冻存细胞复苏后第二天Z好更换一次培养基；5)培养箱隔一段时间彻底用酒精棉擦洗一次；6)养细胞的教训：不能偷懒!细胞虐我千百遍，我待细胞如初恋。1)不管买的细胞、惠赠的细胞，刚拿到手赶紧做两件事：检测是否有支原体污染; 迅速扩增冻存；2)发现有污染迅速处理掉，特别是当你养了很多种细胞时; 细菌污染，真菌污染很容易发现，支原体污染的话，细胞会长的慢，买个支原体检测的kit定期检测一下；3)传代细胞日常的值日清洁是必须的，此外还要定期熏蒸；4)细胞的传代一定要规律，保持相同的confluency和传代时间间隔，不要随心所欲或者拖延；5)注意个人卫生。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-BroMo-2-nitroaniline
FMoc-D-Tyr(tBu)-OH
3-ACETYLACRYLIC ACID
ETHYL (PHENYLTHIO)ACETATE
α-(TriMethylsilyloxy)phenylacetonitrile
BisMuth nitrate oxide
3-(2,6-Dichlorophenyl)-5-methylisoxazole-4-carboxylic acid, 97%
3-[3-(4-morpholinyl)propoxy]benzaldehyde(SALTDATA: HCl)
Hinokitiol
2-azabicyclo[2.2.1]hept-5-ene(SALTDATA: FREE)
2,6-Dibromo-4-fluoroanisole
MEDROXYPROGESTERONE
5-BROMOTHIOPHENE-2-BORONIC ACID
4-Chlorobenzotrifluoride
LithiuM borohydride
2-AMino-5-fluorobenzoic Acid
2,6-DiMethylphenoxyacetic Acid
2-AMino-N-cyclohexylacetaMide
6-Morpholin-4-yl-pyridine-3-sulfonyl chloride, 97%
1,2-DINITROBENZENE
Acid Violet 3
Tri-n-propylsilane
Cyclopropyl 4-methoxyphenyl ketone
4-CHLORO-2-(TRIFLUOROMETHYL)PYRIMIDINE
1,6-Dihydroxy Naphthalene
2,2'-DICHLOROBENZIL
3',4'-DICHLOROACETOPHENONE
CalciuM 2-Ethylhexanoate
6-(DiethoxyMethyl)uracil, 98%
1-Phenyl-1-pentanol, 95%
SodiuM dihydngen phoshate anhydrous
3-Fluoro-4-pyridineboronic acid pinacol ester
9-(BROMOMETHYL)ACRIDINE
Nervonic acid
DiMethyl 2-(1,3-Dithiole)phosphonate
2,5-DibroMoterephthalic acid diethyl ester
N-(3-Formyl-4-pyridinyl)-2,2-dimethylpropanamide, 97%
7-Hydroxyindole-2-carboxylic acid
C-Quinolin-3-yl-methylamine
4,6-Decadiyn-1,10-diol
D-GLUTAMINE METHYL ESTER HYDROCHLORIDE
1,2-Bis(diphenylphosphino)ethane nickel(II) chloride
Sodium pyrophosphate decahydrate
DiphenylaMine;N-Phenylaniline;N-PhenylbenzeneaMine
5-Methyl-2-thiophenecarboxylic acid
L-Hydroxyproline
9-Phenanthreneboronic Acid (contains varying aMounts of Anhydride)
(2-Iodoethyl)benzene, 97%
Di-á-hydroxo-bis-[(N,N,N',N'-tetraMethylethylenediaMine)copper(II)]chloride, 98%
H-CYS-4-ABZ-MET-OH
1-BroMo-2-MethoxyMethylnaphthalene
Benzyloxyacetaldehyde diethyl acetal
Furan, 2-[(1,1-dimethylethoxy)methyl]tetrahydro-
4'-Ethylbiphenyl-4-ol
Fluorescent Brightener ER-II
METHYL DL-MANDELATE
Benzo[1,2-b:4,5-b']dithiophene
DECANAMIDE
BIS(CYCLOPENTADIENYL)TUNGSTEN DICHLORIDE
CesiuM titaniuM oxide, 99.85% (Metals basis)
2,4-Dimethylbenzyl chloride
Zinc acetylacetonate hydrate, 98%
Grimicidin A (High Purity), Bacillus brevis
Tri(m-chlorophenyl)phosphine, min. 97%
3-FLUOROBENZAL CHLORIDE
2-Bromoanthracene
2-(CARBOXYMETHYLTHIO)-4-METHYLPYRIMIDINE
Triisopropyl orthoformate
1-Phenethyl-4-piperidone
2,8-DiMethyldibenzothiophene
2-(3-BroMophenyl)-N-t-butylacetaMide
Silver benzoate, 99%
L-GlutaMic Acid gaMMa-Ethyl Ester
Tris(pentafluorophenyl)borane
2-Chloro-6-fluorobenzothiazole
Diphenyl-2-pyridylmethane
Di-p-tolyl sulfone
N-N-Butyldiethanolamine
Porcine HSP-70/HSPA9 (Heat Shock Protein 70) ELISA Kit
Mouse FMS-like tyrosine kinase 3 ligand,Flt-3L ELISA Kit
Human hnRNP E ELISA Kit
Human D3R/DRD3 (Dopamine Receptor D3) ELISA Kit
Monkey MYO (Myoglobin) ELISA Kit
Rat Interleukin 13,IL-13 ELISA Kit
Chicken major histocompatibility complex,MHC/B ELISA Kit
Human PR3Ab ELISA Kit
Human PDGFC ELISA Kit
βAP ELISA Kit
Modified CCD Agar Base
Modified CCD Agar Base
LutetiuM(III) acetate hydrate, REacton|r, 99.9% (REO)
Crotonyl chloride, 90+%, reMainder Mainly cis-isoMer
4-n-Butylaniline, 97%
2-Phenoxyethanol, 94%
Tri-n-propylsilane
Levamisole
Procion Brilliant Blue
Isonicoteine
2,6-Dimethylpiperidine
Potassium tartrate
4',5'-dichloro-3',6'-dihydroxyspiro[isobenzofuran-1[3H]-9'-[9H]-xanthene]-3-one
Zinc 2-ethylhexanoate, ca 80% in Mineral spirits (17-19% Zn)
4-DiMethylaMinobenzoyl chloride, 97%
Fenthionsulfoxide
2,4-Dihydroxybenzophenone
N-Acryloylsarcosine Methyl ester, 97%, stab. with 250ppM 4-Methoxyphenol
(S)-(-)-1-PHENYLPROPYL ISOCYANATE
2-(4-BROMOPHENYL)-1,1-DIPHENYLETHYLENE
2-Chloro-4-(trifluoroMethyl)pyriMidine
3-Amino-4-fluorophenol 99%
Tetra-n-decyloxysilane
N-(2-Carboxyphenyl)glycine, 97%
2-(1,3-thiazol-4-yl)ethanamine(SALTDATA: 2HCl)
