LLC细胞株：小鼠肺癌细胞系传代培养 货号：GD-C8118672
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4'-Ethylacetophenone, 97%
Pseudolaric Acid B
N-tert-Butoxycarbonyl-N'-benzyloxycarbonyl-L-ornithine
DL-Phenylalanine
BARIUM FERROCYANIDE
METHYL 10,12-PENTACOSADIYNOATE
LithiuM hydride
5-Bromo-2-nitro-benzoic acid
6-Phenylnicotinaldehyde , 97%
1H-Benzimidazole-2-carboxylic acid
Tris(6,6,7,7,8,8,8-heptafluoro-2,2-dimethyl-3,5-octanedionate)lanthanum(III), 99% (99.9%-La) (REO) [La(FOD)3]
2-FLUORO-6-METHOXYBENZOIC ACID
1,5-Bis(diphenylphosphino)pentane
-2-CARBOXYLIC ACID
(S)-Oxiranemethanol
Tris(diMethylaMino)borane
2,3-Dicyano-1,4-dihydroxy-5-nitronaphthalene
5-BroMo-2-propoxypyridine
SodiuM thiosulfate, 98.5%, extra pure, anhydrous
Malonic Acid, Reagent
Xylosucrose
carbamoyl chloride;Carbamyl chloride
4-Chloro-6-methoxyquinoline
N-ethylformamide
n-Butyronitrile
5-(3-Bromophenyl)-1H-tetrazole
Methacrylic Acid Ethyl Ester
1-(6-Chloro-3-pyridazinyl)piperidine-4-carboxaMide
4-AMinobenzeneboronic acid hydrochloride, 97%
DISODIUM HYDROGEN CITRATE SESQUIHYDRATE
Tetrabutyl titanate
4-AMINOBENZOIC ACID SODIUM SALT
1-BroMo-2-Methoxyethane
Antimony(III) fluoride, 98%
2,2-Dimethylhexane
9-oxa-2-azaspiro[5.5]undecane(SALTDATA: FREE)
4-FLUORO-2-HYDROXYBENZALDEHYDE
1-Phenyl-1,2,3,4-tetrahydro-isoquinoline
Fmoc-3,3-diphenyl-L-alanine
Formylhydrazine
Naphthol AS-BI phosphate
Cyclobis(paraquat-1,4-phenylene) Tetrakis(hexafluorophosphate)
4-(tert-ButoxycarbonylaMino)-2,6-diMethylphenylboronic acid
Iodoethane, 98%, pure, stabilized with silver
6-Chloro-1,3-diMethyluracil, 99%
N-EthoxycarbonylphthaliMide [for Peptide Synthesis]
C.I. Pigment Red 4
1-bromo-3-methylbutane
(4S)-3-[(5R)-5-(4-FLUOROPHENYL)-5-HYDROXYPENTANOYL]-4-PHENYL-1,3-OXAZOLIDIN-2-ONE
Dorzolamide
3-Hydroxyisoquinoline
1-t-Butyl-6-fluorobenzoiMidazole
N-BOC-L-Prolinal
3-BroMo-1H-indazole-5-carboxylic acid
1-Boc-2-Methyl-4-piperidinone
m-Tolylacetic acid ,99%
Tetraethylammonium fluoride dihydrate 98%
6-bromo-2-methyl-3(2H)-pyridazinone(SALTDATA: FREE)
1-Chlorododecane
N-PENTAFLUOROBENZOYLIMIDAZOLE
HEXAKIS(BROMOMETHYL)BENZENE
Clopidogrel Related CoMpound A;(S)-(o-chlorophenyl)-6,7-dihydrothieno[3,2-c]pyridine-5(4H)-acetic acid, hydrochloride
2-Oxo-2,3-dihydro-1H-benzoiMidazole-5-boronic acid
1-(2-AMinoethyl)-4-Methyl Hydrochloride
Benzothiazole-6-boronic acid, pinacol ester
4'-Hydroxychalcone, 98%
Methyl-2,3,4-tri-O-benzyl-L-fucopyranose, 98%
PHENYLTHIOHYDANTOIN-ASPARTIC ACID
AMMoniuM AMidosulfate
triethoxyfluorosilane
7-CHLORO-1,2,3,4-TETRAHYDRO-BENZO[B]AZEPIN-5-ONE
Boc-D-Glu-OtBu
Ethyl 1-trityl-1H-imidazole-4-carboxylate
Porcine insulin-like growth factors 2,IGF-2 ELISA Kit
IL-2sR ELISA Kit
TRAIL ELISA Kit
Human ALPL ELISA Kit
GS ELISA Kit
CREB ELISA Kit
Monkey NTXI (Cross Linked N-telopeptide of Type I Collagen) ELISA Kit
CFB ELISA Kit
Human cardiac ankyrin repeat domain 1,ANKRD1 ELISA Kit
CPAF ELISA Kit
Martin-Lewis Agar Base
Select APS Super Broth Base
Tri-n-butyl orthoforMate, 97%
LithiuM peroxide, 90% Min
5-Methyl-2-furoyl chloride, 97%
2-Methylvaleric acid, 98+%
carbamoyl chloride;Carbamyl chloride
Cis-Hexahydrophthalimide
Tracid Brilliant Red B
1,3-Dichloro-5,5-dimethylhydantoin
2,4-Dibromophenol
Ethyl propionylacetate
Sunitinib
6-(4-Fluorophenyl)iMidazo[2,1-b]thiazole-5-carboxaldehyde, 99%
(R)-(+)-1-(4-BroMophenyl)ethylaMine, ChiPros, 99%, ee 98%
4-Methylbenzyl broMide
4-Fluoro-2-(trifluoroMethyl)benzaldehyde
(2-BROMOETHYL)TRIMETHYLSILANE
TetraMethylguanidine
6,6-DIMETHYLBICYCLO(3.1.1)HEPT-2-ENE-2-ETHANOL
3-NORADAMANTANECARBOXYLIC ACID
Phenyl trifluoroacetate 98%
Diphenylamine Sulfate
4-Fluoro-2-methoxybenzyl alcohol
5-Methyl-1-phenylpyrazole-3-carboxylic acid
