OCM-1人眼脉络膜黑色素瘤细胞系液体培养基 货号：GD-CP138268
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤：1)选择处于对数生长期的细胞，在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉，用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶，加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞，使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min，10分钟)；2)去上清液，加入含20%小牛血清的完全培养基，于4℃预冷15分钟后，逐滴加入已无菌的DMSO或甘油，用吸管轻轻吹打使细胞均匀，细胞浓度为5×106～1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢？答案是不一定的，具体要看培养的细胞类型来选择；3)将分装上述细胞悬液于2ml冻存管中，每管0.25ml。冻存管要将盖子盖紧，并标记好细胞名称和冻存日期，同时作好登记(日期、细胞种类及代次、冻存支数)；4)将装好细胞的冻存管放到冻存盒中，-80℃冰箱过夜。；如果有程序降温器(放在-80℃冰箱过夜，放入液氮罐)Z好;或者可以在4℃，2h;然后转到-20℃，2h;-80℃，2h;放入液氮罐；5)细胞冻存在液氮中可以长期保存，但为妥善起见，冻存半年后，Z好取出一只冻存管细胞复苏培养，观察生长情况，然后再继续冻存。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2(1H)-Quinolinone-3-carboxaldehyde, 97%
2-Chloroadenine
2,5-DiMethoxycinnaMic acid
2-(4-BroMo-3,5-diMethylphenoxy)acetonitrile
SodiuM 2-ethylhexanoate
14-Carboxytetradecyl 15-hydroxypentadecanoate
3-(PHENYLSULFONYL)PROPIONITRILE
cis-2,6-Dimethylaminoethylmorpholine
7-Amino-2H-1,4-benzoxazin-3(4H)-one
(Hydrazinocarbonyl)ferrocene [for HPLC Labeling]
5-(Trifluoromethyl)-2-furoic acid
3,3',4',5,7-Pentahydroxyflavone
3',4'-DIMETHOXYFLAVONE
2-AMINO-4-HYDROXY-6-(TRIFLUOROMETHYL)PYRIMIDINE
2,3-DIHYDROXYQUINOXALINE
4-(2-Hydroxyethyl)benzonitrile
Diethylene Glycol MonoMethyl Ether Methacrylate (stabilized with MEHQ)
3-BroMo-2-Methoxybenzoic acid
PlatinuM(IV) chloride, 99%
Fast Red Violet LB Salt
5-Norbornene-2,3-diMethanol
2-Benzoylthiophene
Acid Blue 45
2-hydroxyisocaproic acid
Pyridoxine
2-Hydroxypyrimidine
CIS-1,3-DIMETHYLCYCLOHEXANE
TributylaMine
NeodyMiuM(III) oxide, REacton|r, 99.999% (REO)
^b-Cyclocitral, 90+%
Maltotriose
DiMethoxydiphenylsilane
2-Dicyclohexylphosphino-2',6'-diMethoxybiphenyl
Z-LYS(BOC)-OH
3-MERCAPTO-1-PROPANOL
PHENYLHYDROQUINONE DIACETATE
4-(Trifluoromethoxy)phenylacetonitrile
3-Chloro-2-fluorobenzenesulfonamide
Methyl (R)-3-hydroxy-3-phenylpropanoate, 99%
4,4'-Bis(4-amino-1-naphthylazo)-2,2'-stilbenesulfonic Acid [Indicator for Organic Acid Anhydrides by morpholine method]
Fluorescein isothiocyanate isomer I
Poly(dimethylsiloxane)
alpha-Methyldopa sesquihydrate
o-Phenanthroline monohydrochloride monohydrate
Ethyl 4-broMocrotonate
Bis(benzonitrile)palladiuM(II) dichloride
Methyl 6-Fluoro-2-naphthoate
4-AMinocyclohexanol
2-[4-(Methoxycarbonyl)-5-Methyl-2-oxo-2,3-dihydro-1H-pyrrol-3-yl]acetic acid, 97%
Sodium hexafluorophosphate
2,6-DiaMinopurine Hydrate
Tartrazine
C.I.Pigment Red 122
Palmiticacidhydrazide
2-Amino-5-chloro-2'-fluorobenzophenone
3-CHLORO-2-METHYLACETANILIDE
2,3,6-TRIMETHYLQUINOXALINE
1,4-Diethoxybenzene
2-Mercapto-4,6-diMethylpyriMidine hydrate
5-Bromo-3,4-dimethylbenzene-1,2-diamine , 90%
Ethyl 6-Methyl-2-oxo-3-cyclohexene-1-carboxylate (mixture of isomers)
6-Bromo-4-chloro-8-fluoro-quinoline-3-carbonitrile
Diethylene Glycol Diacetate
2-Bromochlorobenzene
NICOTINOYL CHLORIDE HYDROCHLORIDE
4-Acetamidobenzenesulfonyl azide
(S)-(-)-2-Methyl-1-butanol
5-Fluorooxindole
(-)-cis-2-BenzylaMinocyclohexaneMethanol
3-BroMo-4-Methylthiophene-2,5-diboronic acid, pinacol ester
9-Phenanthrenecarboxaldehyde
4-BroMobenzenesulfonyl chloride, 98%
2-AMinonicotinic Acid
Methoxycyclohexane
Zinc formate
Dodecylbenzenesulfonic Acid
3-Formylphenylboronic acid
cis-4-Aminoadamantan-1-ol
TPA ELISA Kit
LAMα1 ELISA Kit
Human TPS ELISA Kit
Human Bone morphogenetic protein 7,BMP-7 ELISA Kit
NTx ELISA Kit
VTG ELISA Kit
ACE2 ELISA Kit
Human GAL12 (Galectin 12 ) ELISA Kit
Human CYB561 ELISA Kit
sCD44s ELISA Kit
Zobell Marine Broth 2216
Murashige & Skoog Medium
Isopropyl palmitate
SULFAPHENAZOLE
5-BroMo-2-chloropyridine-3-boronic acid, 95%
2-Hydroxynicotinic acid
2-Benzoylthiophene
3,4,6-Trichloropyridazine
3a,6a-Diphenylglycouril
Propionamide
3-Glycidoxypropyltrimethoxysilane
Deoxycorticosterone acetate
2-Vinylpyridine, 97%, stab. with 0.1% 4-tert-butylcatechol
2-n-Pentylpyridine, 98%
4-Chloro-1,2-difluorobenzene, 98%
Protodioscin
N,N-DiMethylforMaMide
TETRAHYDROLAVANDULOL
4-(3,3,4,4,5,5,6,6,7,7,8,8,8-Tridecafluorooctyl)benzyl alcohol
4-Chloro-3-Methylphenol
3,4-Methylenedioxy-beta-nitrostyrene
3-(3,4-Dimethoxyphenyl)propionic acid
Decyl aldehyde, 95%
1,2,3,4-Tetrahydro-quinoline-2-carboxylic acidmethyl ester
N-Biotinyl-N'-(3-maleimidopropionyl)-3,6-dioxaoctane-1,8-diamine
