MuM-2B人侵袭性脉络膜黑色素瘤细胞系液体培养基 货号：GD-CP139047
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
(S)-(-)-2-AMino-2-Methyl-4-pentenoic acid, 99%, ee 99+%
9-Benzylcarbazole
2-(DIPHENYLPHOSPHINO)ETHYLTRIETHOXYSILANE
6-Nitroquinazolin-4(3H)-one
4-Methoxy-N-(4-fluorobenzylidene)aniline
1-BroMo-3-n-hexylbenzene, 97+%
2,5-DibroMothiophene
(S)-beta-(2-chlorophenyl)alanine
Trimethylsilylmethane thiol
2,4,5-Trichlorobenzenesulfonic Acid Hydrate
(R)-(+)-2-(2'-Carbomethoxyethyl)-2-methylcyclohexanone, 98.5+%
5-Chloro-2-methyl-4-nitroaniline
2,3,4-TRICHLOROBENZENESULFONYL CHLORIDE
L-Alanine benzyl ester 4-toluenesulfonate
4-BroMothioanisole
3-BroMo-2-(broMoMethyl)propionic acid
Diethylene Glycol Monobenzyl Ether
Ethyl 8-broMooctanoate
Trifluoroacetic acid lithiuM salt, 95%
PotassiuM Thiosulfate
N-(HydroxyMethyl)nicotinaMide
3,5-diiodoanthranilic acid
L-Born-2-yl acetate
M-chloroperoxybenzoic acid
1-bromo-7-fluoroheptane
ERBIUM (III) 2,4-PENTANEDIONATE
Thymol Blue
4-(TriMethylsiloxy)benzaldehyde, 97%
2-AMinoindane hydrochloride, 98%
TriMethylhydroquinone, 98%
Methabenzthiazuron
Pyridine-4-carboxaldoxiMe, 97%
4-(3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-Heptadecafluorodecyl)benzylaMine
PotassiuM acetate hydrate, Puratronic(R), 99.997% (Metals basis)
1,4-BIS(BROMOMETHYL)NAPHTHALENE
Isobutyl (R)-(+)-lactate
N-Allylmethylamine
3-Nitrophenylsulfur Pentafluoride
2-phenylacetohydrazide(SALTDATA: FREE)
Ethyl (R)-(-)-2-pyrrolidone-5-carboxylate, 99%
2,2'-Bis(trifluoromethyl)benzidine
Eriochrome Black T
Leaf alcohol
Silicon tetrachloridev;Tetrachlorosilane
2-(DiMethylaMino)ethyl Methacrylate
Direct Blue 1
3-BroMo-2-(2'-fluorobenzyloxy)phenylboronic acid
5-Nitro-M-xylene, 97%
4-(EthylaMino)benzoic acid, 97%
Minoxidil
2-Hydroxy-4-Methyl-3-nitropyridine
3,4-dinitrobenzoic acid
Fmoc-4-phenyl-Phe-OH
2,5-Difluoropyridine
Gestodene
2,4-DINITROBENZENESULFONIC ACID
Ethyl (2-fluorobenzoyl)acetate
7-Methoxyindole-2-boronic acid pinacol ester
N-Carbobenzyloxy-L-valine
4-Chloropyridine-3-boronic acid, 97%
4-tert-Amylphenol, 99%
Methyl 3,3-Dimethyl-4-pentenoate
3,5-Dibromobenzeneboronic acid 98%
Sodium diethyldithiocarbamate
2-Chloro-5-trifluoromethylbenzaldehyde
CHLOROMETHYL THIOCYANATE
MOLECULAR SIEVES
1,3-DiMethyl-1H-pyrazole-5-carbaldehyde
1,6-Heptadien-4-ol
3-HydroxyMyristic Acid
Boc-d-abu-oh
4-(2-Methyl-1,3-thiazol-4-yl)benzoic acid, 97%
STRONTIUM SULFIDE
DiMedone
(1α,2α,3β,6β)-1,2,3,6-tetrahydro-3,6-methanophthalic anh...
3-methylisoxazol-5-amine
1,5-Dichloro-2,4-dinitrobenzene
2,6-Dichlorobenzonitrile
CK 13 ELISA Kit
α-MSH ELISA Kit
Human MUC12 ELISA Kit
Human LRP ELISA Kit
BMP2 ELISA Kit
S100A12 ELISA Kit
Chicken Interleukin 3,IL-3 ELISA Kit
Human DSIP ELISA Kit
Human VEGFD ELISA Kit
SRP9 ELISA Kit
Anaerobic Agar
Yeast Synthetic Drop-out Agar Medium
Isobutyl Isobutyrate
TRIMYRISTIN
5-Chloro-8-hydroxy-7-iodoquinoline, 98%
PotassiuM 2-fluorophenyltrifluoroborate, 96%
3,5-diiodoanthranilic acid
Solvent Yellow 21
Palladium(II) iodide
7-Fluoroindole
(+)-1,2-BIS((2S,5S)-2,5-DIETHYLPHOSPHOLANO)BENZENE(CYCLOOCTADIENE)RHODIUM (I) TRIFLUOROMETHANESULFONATE
Cresol Purple
4-AMino-5-BroMo-2-Chloropyridine
N-Boc-2-fluoro-L-phenylalanine, 98%
3',4'-DiMethylacetophenone, 98%
1,3-Dichloro-2-butene, cis + trans, 98%, stab. with BHT
4-BroMothiophenol
Benzophenone-4,4'-dicarboxylic Acid , 95%
MANGANESE SELENIDE
BIS(2-HYDROXYETHYL)DITHIOCARBAMIC ACID ZINC SALT
BISMUTH OXYCHLORIDE
Lead(II) chroMate, ACS, 98%
3-Chloro-4-fluorophenylboronic acid
Lithium tetrachloropalladate(II) hydrate, 99%
2-Fluoro-4'-methoxybenzophenone
