SCC-090 Cell：人口腔鳞癌细胞系(来源于ATCC等知名细胞库) 货号：GD-C6818284
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤：1)选择处于对数生长期的细胞，在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉，用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶，加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞，使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min，10分钟)；2)去上清液，加入含20%小牛血清的完全培养基，于4℃预冷15分钟后，逐滴加入已无菌的DMSO或甘油，用吸管轻轻吹打使细胞均匀，细胞浓度为5×106～1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢？答案是不一定的，具体要看培养的细胞类型来选择；3)将分装上述细胞悬液于2ml冻存管中，每管0.25ml。冻存管要将盖子盖紧，并标记好细胞名称和冻存日期，同时作好登记(日期、细胞种类及代次、冻存支数)；4)将装好细胞的冻存管放到冻存盒中，-80℃冰箱过夜。；如果有程序降温器(放在-80℃冰箱过夜，放入液氮罐)Z好;或者可以在4℃，2h;然后转到-20℃，2h;-80℃，2h;放入液氮罐；5)细胞冻存在液氮中可以长期保存，但为妥善起见，冻存半年后，Z好取出一只冻存管细胞复苏培养，观察生长情况，然后再继续冻存。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-(2,3-DiMethylphenylcarbaMoyl)benzeneboronic acid, 98%
4-BroMo-2,6-difluorobenzyl alcohol
1-Hydroxydicyclopentadiene , 97%
2,3-DiMethoxybiphenyl-3-carboxylic acid
Bis(triMethylenedithio)tetrathiafulvalene [Organic Electronic Material]
4-(ChloroMethyl)tolunitrile
1-BENZOYL-2-THIOUREA
Chloro(indenyl)bis(triphenylphosphine)ruthenium(II), dichloromethane adduct, min. 98%
(S)-3-(4-Benzyloxy-phenyl)-2-hydroxy-propionicacid methyl ester
3,4-Dibromo-1H-indole, N-BOC protected 98%
N-(4-fluorobenzyl)cyclohexanamine(SALTDATA: HCl)
Biphenyl-4,4'-dicarboxylic acid
4-Aminodiphenylamino sulfate
NICKEL SULFATE
4-Chlorocinnamic acid
2-Benzylbenzyl alcohol
2,3-Dihydrobenzofuran
1-BroMo-4-Methoxybutane
Poly(ethylene glycol Methyl ether), average M.W. 750
Ferric Subsulfate Solution, USP
4-Chloro-6-MethoxypyriMidine
N,N'-dithiodi-o-phenylenedibenzamide
Imidazole, 1-methyl-4-nitro-
ethyl O-acetylsalicylate
tris(2-ethylhexyl) phosphate
4-N-NONYLOXYANILINE
2-Fluoroanisole
(S,S)-(+)-2-Diphenylphosphino-1,2-diphenylethylaMine, 97+%
1-(Cyclohexylcarbonyl), 97%
N-Boc-D-phenylalanine Methyl ester, 98%
2,5-DiMethyl-2,5-di(tertiary-butylperoxy)-hexyne-3
4'-Octyl[1,1'-biphenyl]-4-carbonitrile
1,1,1-TRIFLUOROACETONE CYANOHYDRIN
Zinc phosphate
5-BroMo-2-Methylbenzaldehyde
4-AMINOHIPPURIC ACID
2-(1-Aminoethyl)thiophene
2H-Perfluoroprop-1-ene 97%
3,9-Bis(2-cyanoethyl)-2,4,8,10-tetraoxaspiro[5.5]undecane
4-(Hydroxyamino)quinoline N-Oxide
TRIS(4-TRIFLUOROMETHYLPHENYL)PHOSPHINE
1,7-HEPTANEDIOL
5-Methylindole
DiMethylthiocarbaMoyl chloride;
Ethyl acetiMidate hydrochloride
2-BroMo-5-hydroxybenzaldehyde
3-Fluoropyridine-4-boronic acid hydrate
DiMethylaMinoacetaldehyde diMethylacetal, 97%
PalladiuM(II) broMide, 39.8-40.1% Pd
2-BroMo-5-nitrofuran
2-Heptyne
Dimethylcyanamide
6-chloro-5-(trifluoromethyl)-3-Pyridinemethanol
methyl 3-formyl-2-nitrobenzoate
Luxabendazole
Butyrylferrocene
1-(4-N-BUTYLPHENYL)-2-(4-METHOXYPHENYL)ACETYLENE
Tris(dibenzylideneacetone)dipalladiuM-chloroforM adduct
5-BroMo-2,4-diMethoxybenzoic acid
N-Hydroxy-2-(4-methyl-thiazol-2-yl)acetimidamide
4-Methyl-oxazol-2-ylamine
2-Chloro-4-nitrobenzoyl chloride
Vinyl Sorbate (stabilized with MEHQ)
1-PYRIDIN-3-YLMETHYL-
DELTA-DODECANOLACTONE
N-NONADECANOIC ACID ETHYL ESTER
Copper(ic)chloride;Copper dichloride;Copper bichloride;Cupric chloride
1,5-Pentanedithiol
(+)-4,5-Bis[hydroxy(diphenyl)Methyl]-2-Methyl-2-phenyl-1,3-dioxolane
1-(2-Methoxyphenoxy)-2-propanol
H-Dab(boc)-oMe hcl
(S)-(-)-2-(CarbobenzyloxyaMino)-3-phenyl-1-propanol, 98%
Spermidine
6-AMinouracil
Nicotinoyl chloride
2-Amino-2-deoxy-D-glucose sulfate
Polyoxyethylene stearate
Trihexylphosphine
Porcine Histamine,HIS ELISA Kit
OPAs ELISA Kit
Human CTSW ELISA Kit
BMP2 ELISA Kit
RAD6 ELISA Kit
IL-1β ELISA Kit
VCAM-1 ELISA Kit
GAL8 ELISA Kit
Human CYC1 ELISA Kit
STLR-6 ELISA Kit
Brain Heart Infusion Agar
Holley-Baker Medium
Perillartine
Flavin adenine dinucleotide disodium salt
5-BroMo-2-Chloro-3-Nitropyridine
2'-HYDROXYFLAVANONE
N,N'-dithiodi-o-phenylenedibenzamide
3,4-Dichlorophenylacetone
Estra-4,9-Diene-3,17-Dione
Isobutyryl chloride
4-(Dimethylamino)cinnamaldehyde
2,3,5-Trimethylphenol
Genistein
Chlorhexidine digluconate, 20% w/v aq. soln., non-sterile
4-(2-Methyl-3-furylthio)-5-nonanone, 95%
Anisole
2-MethoxybenzylaMine
3,3-Bipyridine-6,6-dicarbonitrile
6-BroMo-4-Methyl-1,4-benzoxazin-3-one
METHACHOLINE CHLORIDE
3-CHLORO-5-FLUOROBENZALDEHYDE
1,2-Cyclohexanedione
2-Ethylhexanoyl Chloride
(1R)-endo-(+)-Fenchol, 96%
1,1-Difluoroethane (FC-152a) 99%
