G401 Cell：人肾癌Wilms细胞系(来源于ATCC等知名细胞库) 货号：GD-C6819074
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Isoeugenol, cis + trans, 98+%
2,3,5-Trifluorophenylacetic acid
3-Methylsalicylic acid
2-Methyl-2-propenoic acid cyclohexyl ester
3-AMino-4,6-diMethylisoxazolo[5,4-b]pyridine
E-2-(1-PENTENYL)-4,4,5,5-TETRAMETHYL-1,3,2-DIOXABOROLANE
TERT-BUTOXYCHLORODIPHENYLSILANE
4-Cyanobiphenyl
(S)-(-)-Glycidol
3-(Benzylcarbamoyl)-5-fluorobenzeneboronic acid 98%
4-Butylphenol
GLYCINE ANHYDRIDE
L-(-)-Histidinol dihydrochloride
3-Chloro-4-fluoronitrobenzene
4-(BroMoMethyl)tetrahydropyran
2,6-Difluorophenyl isocyanate
4-(DiMethylaMino)benzyl Alcohol
N-(Furan-2-ylMethyl) 4-broMo-3-trifluoroMethylbenzenesulfonaMide
TriMethylaluMiniuM, 1.0M solution in heptane, AcroSeal
Propyl Propionate
O-Benzoyl-N-tert-butylhydroxylaMine Hydrochloride
6-Nitrophthalide
L-Serine methyl ester hydrochloride
Phenethyltrichlorosilane
N-phthaloyl-L-glutamic acid
N-(N-PROPYL)ACETAMIDE
4-BROMO-2,6-DIMETHYLANISOLE
L-Histidine
2-(n-Propylthio)nicotinic acid, 97%
2,2-DiMethylbutyric acid, 97%
FMoc-Phe(3,4-Cl2)-OH
Isonicotinic acid
ERIO GREEN B
Poly(vinyl alcohol)
2,4-BIS(TRIFLUOROMETHYL)BENZONITRILE
1,4-Dihydro-2-Methylbenzoic acid
1,5-Dimethyl-1H-pyrazole-3-carboxylic acid ,97%
4-(4-Chlorophenoxy)phenylhydrazine hydrochloride
sec-Butyl Ethyl Ether
Terephthalic-d4 acid, 97 atom% D, for NMR
2-Bromo-6-methoxypyridine
Tris(2-chloroethyl) phosphate
L-Arginine L-glutamate
Octacosane
SodiuM chlorite
2-(Methylthio)phenol
Pentyl Valerate
1,2-Diiodobenzene, 98%
5-Fluoro-2-Methoxyphenylboronic acid, 98%
N-Methyl pyrrole
3,4-Hexanedione
Zirconium oxysulfate
4-Hydroxy-6-Methylnicotinic Acid
Tris(1-chloro-2-propyl) phosphate
3-methylcinnamic acid
Tropinone
3-AcetaMidoacetophenone, 98%
3-Cyanobenzyl broMide
2-(2-Pyridyl)ethanesulfonic Acid , 95%
4-(2-Tetrahydro-1H-pyrrol-1-ylethyl)pyridine 97%
N-(2,6-Diethylphenylcarbamoylmethyl)iminodiacetic Anhydride
1,3,5-Tri-O-benzoyl-2-deoxy-2-fluoro-α-D-arabinofuranose ,98%
Benzyl Methacrylate (stabilized with MEHQ)
Gluconic acid
FMOC-(S)-3-AMINO-4-(3-CHLORO-PHENYL)-BUTYRIC ACID
4-Iodo-1,2-dimethylbenzene
Testosterone
3-(4-Methylpiperazin-1-yl)benzonitrile
10-BroMo-1-decene
4-Cyano-3-(trifluoroMethyl)phenylboronic acid
ChroMoxane Cyanine R, pure
[2-(2-Furyl)phenyl]Methanol, 97%
4-(DiMethylaMino)butyraldehyde DiMethyl Acetal
tert-ButylMagnesiuM Chloride (23% in Tetrahydrofuran, ca. 2Mol/L)
7-Methylindole
2-Amino-3,5-difluoropyridine
2-fluoro-3-(trifluoromethyl)benzoic acid
(3s,4r)-4-acetoxy-3-[(r)-1-(tert-butyldimethylsilyloxy)ethyl]azetidin-2-one
SEPP1 ELISA Kit
αNAG ELISA Kit
Human PML ELISA Kit
Human SP-A ELISA Kit
BSA ELISA Kit
Rat Smad4 (Mothers Against Decapentaplegic Homolog 4) ELISA Kit
PD ELISA Kit
Human secretin ELISA Kit
Human Vascular Endothelial cell Growth Factor B，VEGF-B ELISA Kit
AT1R-Ab ELISA Kit
HTM Medium Base
Gelatin PBS Broth
Isobutyric acid
Glycyl diphenylborinate, 95%
5-Chloro-2-pentanone, 94%
2-Fluoro-N-n-propylbenzaMide, 97%
6-Nitrophthalide
Disperse Red B-BL
Tetraethylene glycol
4-Methoxy-3-(trifluoromethyl)aniline
Methoxypropiophenone
N,N-DIMETHYL-5-METHYLFURFURYLAMINE
2-BroMonicotinic Acid
2'-Fluoro-4'-(trifluoroMethyl)acetophenone, 97%
2-Nitrobenzyl broMide, 98+%
4'-Chloroacetophenone, 98+%
PotassiuM cyanate
2,5-Bis(4-biphenylyl)thiophene
BIS(2,2,2-TRIFLUOROETHYL) PHTHALATE
(+)-Longicyclene
2-BroMo-5-chloropyridine-4-boronic acid
(2-Vinylphenylboronic acid, propanediol cyclic ester
1-(2-Chloro-6-fluorophenyl)cyclohexanecarboxylic acid, 97%
2-[2-(Methylamino)ethyl]pyridine
Fmoc-D-homocyclohexylalanine
