293F人胚肾上皮细胞系专用培养基

293F人胚肾上皮细胞系专用培养基 货号：GD-CP128301

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤：1)选择处于对数生长期的细胞，在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉，用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶，加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞，使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min，10分钟)；2)去上清液，加入含20%小牛血清的完全培养基，于4℃预冷15分钟后，逐滴加入已无菌的DMSO或甘油，用吸管轻轻吹打使细胞均匀，细胞浓度为5×106～1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢？答案是不一定的，具体要看培养的细胞类型来选择；3)将分装上述细胞悬液于2ml冻存管中，每管0.25ml。冻存管要将盖子盖紧，并标记好细胞名称和冻存日期，同时作好登记(日期、细胞种类及代次、冻存支数)；4)将装好细胞的冻存管放到冻存盒中，-80℃冰箱过夜。；如果有程序降温器(放在-80℃冰箱过夜，放入液氮罐)Z好;或者可以在4℃，2h;然后转到-20℃，2h;-80℃，2h;放入液氮罐；5)细胞冻存在液氮中可以长期保存，但为妥善起见，冻存半年后，Z好取出一只冻存管细胞复苏培养，观察生长情况，然后再继续冻存。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-Fluoro-4-[Methoxy(Methyl)carbaMoyl]benzeneboronic acid, 98%
N-Boc-nortropinone
ALLYLTRIETHOXYSILANE
2-PHOSPHONOBUTYRIC ACID
3-PHTHALIMIDOPROPIONIC ACID
5-Methyl-1H-benzotriazole
N-Ethyl-N-(4-Methoxybenzyl)benzenesulfonaMide
Boc-4-oxo-Pro-OMe
(E)-3-(5-Iodo-2-furyl)prop-2-enoic acid , 97%
Benzoin Isobutyl Ether
4-(tert-Butoxycarbonyl)benzeneboronic acid
DIDECYL DISULFIDE
3,4-Dichlorobenzotrichloride
PENTACHLOROTHIOANISOLE
Ethyl 2-oxocyclohexanecarboxylate
4-PiperidineMethanol
1,5-Di-p-tolyl-3-Methyl-1,4-pentazadiene
2-BroMo-3-Methoxy-6-nitropyridine
Polystyrene: 2% divinylbenzene copolyMer beads, 200-400 Mesh
FluoxyMesterone (CIII), USP
2-AMino-5-chloropyridine
Zinc di-n-butyldithiocarbamate
ethyl 3-oxo-3-(3,4,5-trimethoxyphenyl)propionate
7-Methoxycoumarin
1-Bromo-2-methylpropane
4-Chloro-3-nitrobenzenesulfonyl chloride
LACTALBUMIN HYDROLYSATE
2-BroMo-3,3,3-Trifluoropropene
Ethyl (S)-(-)-1-Boc-4-oxopiperidine-2-carboxylate, 95%
2-Fluoropropionic acid, 96%
Cresol Mixed isoMers
3-Fluorobenzaldehyde
Bis[1,2-bis(diphenylphosphino)ethane]palladiuM(0)
Z-D-Dap-OH
Methyl 5-aMino-2-broMo-4-chlorobenzoate
3-BroMo-4-cyanothiophene
2-Bromo-1-[4-(2-thienyl)phenyl]-1-ethanone, 97%
3-Amino-5-ethyl-1H-pyrazole
2,4-Difluorophenacylamine hydrochloride 99%
(S)-(-)-Methylsuccinic Acid
Benzoic anhydride
4-ISOPROPYLPHENYLACETONITRILE
N-Fmoc-N'-(4-methoxy-2,3,6-trimethylbenzenesulfonyl)-L-arginine
TrifluoroacetaMide;2,2,2-TrifluoroacetaMide
Bis(4-broMophenyl)acetylene
1,4-Naphthoquinone
Tetrahydroxy-1,4-benzoquinone DisodiuM Salt
1-Hexanesulfonic acid, sodiuM salt, 99+%, Ion pair chroMatography, anhydrous
4-Pyrazolecarboxylic acid, 97%
2,5-Anhydro-D-mannitol
4-Nitro-1-naphthol
3-Aminophenol hemisulfate salt
1,2-Cyclohexanediamine, N,N'-dimethyl-, (1R,2S)-rel-
3-(1-Adamantyl)propan-1-ol
4-(1H-IMIDAZOL-1-YL)BENZOIC ACID
ETHYLMALONIC ACID
N,N-DIETHYLACRYLAMIDE
3-(TrifluoroMethyl)anisole
ALLYL N-OCTYL ETHER
2-Phenyl-1,3-thiazole-4-carbonyl chloride , 95%
Methyl 4-Chlorocinnamate
1,2,3,4-Tetrahydro-2-naphthol, 97%
Z-3-(2-Naphthyl)-D-alanine
Ethyl 2-oxocyclopentanecarboxylate
ETHYL 2-BENZYLACETOACETATE
5-Fluoro-2-methylpyridine
AMlodipine Besylate
1,3-Thiazole-2-carbonyl chloride
(+)-O-Acetyl-D-Malic Anhydride
4-AcetaMido-3-(trifluoroMethy)phenylboronic acid, pinacol ester
4-ChloroMethyl-2-Methylthiazole hydrochloride, 98%
3,5-Dichloropyridazine, 97%
TRIS(2,2'-BIPYRIDYL)RUTHENIUM(II) CHLORIDE HEXAHYDRATE
2-Fluoro-5-(trifluoroMethyl)phenol
DL-alpha-Methyltryptamine
Acid Black 242
Attapulgite
p-Fluoro-ALPHA-methylphenylacetonitrile
TNPO1 ELISA Kit
Mouse Complement fragment 5a,C5a ELISA Kit
Human cath-G ELISA Kit
OPG ELISA Kit
26S.PSM ELISA Kit
Hamster matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA K
HPX ELISA Kit
Human GAL3ST2 ELISA Kit
Human CYP1B1 ELISA Kit
CC16 ELISA Kit
Bacteria Save Medium
Murashige & Skoog Medium
SodiuM hydroxide, pearl, 97%
NICKEL CYCLOHEXANEBUTYRATE
5-BroMo-2,3-difluorophenol
2-Hydroxyacetophenone
Zinc di-n-butyldithiocarbamate
5-nitrothiophene-2-carboxylic acid
2-Bromo-4-nitroanisole
P-aminotoluene-o-sulfonanilide

2-N-BUTYRYLTHIOPHENE
(S)-(+)-Tetrahydrofurfurylamine
4,5-Difluoro-2-Iodobenzoic Acid
NitrosoniuM hexafluorophosphate, 96%
2,3-DiMethylnaphthalene, 97%
(R)-(+)-4-Isopropyl-2-oxazolidinone
Glucose oxidase
ForMaMidine disulfide dihydrochloride
3-BroMothiobenzaMide
N-PHENYLIMINODIACETIC ACID
CHROMIUM(III) BENZOYLACETONATE
3-Methoxybenzenethiol, 95%
6-Bromochroman 97%
methyl 2,4-dimethyl-1H-pyrrole-3-carboxylate(SALTDATA: FREE)
Cadmium arsenide (99.999%-Cd) PURATREM