U87 MG Cell：人恶性脑胶质瘤传代贴壁细胞系

U87 MG Cell：人恶性脑胶质瘤传代贴壁细胞系 货号：GD-C6620577
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
(^+)-1-Boc-3-broMopyrrolidine, 95%
Profenofos
Ethyl (4-Methylthiophenyl)acetate
3-Fluoro-4-(Methylthio)phenylboronic acid
N-MethylbenzylaMine
SodiuM L-lactate-3-13C solution
3-BroMo-5-Methyl-2-Methoxyphenylboronic acid
Cyclopentanecarboxylic acid, 98+%
2,6-Dichloropyridine
Ethyl 4-methyl-2-(2-thienyl)-1,3-thiazole-5-carboxylate
pyrrolidin-2-ylacetic acid(SALTDATA: HCl)
5-Fluoro-2-methylindole
2-NITROCHALCONE
Pinacidil
N-Methyl-D-aspartic acid
Chlorobenzene
1,3-DiMethyl-2-thiohydantoin
5-BroMopyridine-3-sulfonic acid
Styrene-d8, for NMR, 98+ atoM% D, stabilized
Mercuric Iodide, Red, Powder, Reagent, ACS
8-Methoxy-4-Methylbenzo[g]couMarin
Oxamic acid
tetraethylphosphonium bromide
1,5-diiodopentane
Biurea
(S)-(-)-3-Butyn-2-ol
4-Piperidinopiperidine
2-ChloropyriMidine-5-Boronic Acid
ChlorodiMethyl-3,3,3-trifluoropropylsilane, 96%
4-DiMethylaMinobenzophenone, 98%
Methyl red
NeodyMiuM nitrate hexahydrate
Liothyronine sodiuM
tert-Butyl 2-isocyano-3-phenylpropionate
BariuM chloride dihydrate
ISOPROPYL METHYL SULFONE
Cyclohexylmethyldichlorosilane, 97+%
2-Fluoropyridine-5-boronic acid ,98%
Methyl imidazo[1,2-a]pyridine-8-carboxylate
1-butyl-1H-tetrazol-5-amine(SALTDATA: FREE)
2-Hydroxy-4-(trifluoromethyl)pyrimidine
BEHENIC ACID
Poly(acrylamide)
BOTTLE BEEF EXTRAC
MethylMalonic Acid
CalciuM citrate
(S)-(-)-1-Methyl-2-(1-piperidinoMethyl)pyrrolidine
N,N-Diethyl 2-aMinobenzenesulfonaMide
Doyle dirhodiuM catalyst - RH2(4S-MPPIM)4
Parsley Seed Oil
N,N-DiMethylMethacrylaMide
Ethyll-4-Chloro-3-Hydroxy Butyrate
3-Methylbutyrylcarnitine
3-(phenylsulfonyl)propionic acid
2-cycloheptylethanamine
2-Bromo-3-methylbutyric acid
2-AcetaMido-5-broMopyriMidine
4-HYDROXY-2-(TRIFLUOROMETHYL)BENZOIC ACID
4-AMino-3,5-dichlorobenzotrifluoride
1-Methyl-2-pyrrolidinone, extra pure, 99%
Butadiene monoxide, 97%
3-Bromo-N-isopropylbenzenesulphonamide 98%
Acetylcholine Perchlorate
Boc-L-Histidine(Tosyl)
DL-4-HYDROXY-3-METHOXYMANDELIC ACID
3,5-Dihydroxy-4-methylbenzoic acid
2-Methylcyclohexanol
N-AMinophthaliMide
1-(3-Chlorophenyl)-4-(3-chloropropyl) Hydrochloride
1-Propylpyrazole-4-boronic acid
Hydroxylapatite, for analysis
(-)-Lupinine, 97%
3-(TrifluoroMethoxy)benzoyl Chloride
2-Fluoro-3-hydroxypyridine
P-tolualdehyde 2,4-dinitrophenylhydrazone
Dicyclohexylamine
1-(1-benzofuran-3-yl)-2-bromo-1-ethanone
Breviscapin
PICP ELISA Kit
IL21 ELISA Kit
Human TNF α converting enzyme,TACE ELISA Kit
Human GAPDH ELISA Kit
IL6 ELISA Kit
Rat Cluster of differentiation 3,CD3 ELISA Kit
IL1A ELISA Kit
OPAs ELISA Kit
ANF ELISA Kit
Nephrin ELISA Kit
Salmonella Shigiella Enrichment Broth
Mn2+ Nutrient Agar
TriMethyl Orthobenzoate
Potassium persulfate
5-Hexyn-1-ol, 97%
2-Methylpiperidine, 99%
Oxamic acid
Piperine
(-)-ambroxide
4-Methyl-2-nitrophenol

2-Hydroxybenzimidazole
3-TRIMETHYLSILYL-2-PROPYN-1-OL
Methyl Glycolate
1,4-Difluoro-2,5-diMethoxybenzene, 97%
5-Chlorovanillic acid, tech. 90%
1-Octen-3-ol
1-MethyliMidazole-5-carboxylic Acid
2-AMinopyriMidine-5-boronic acid
3-Indoleethanol
DIMETHYL PIMELIMIDATE DIHYDROCHLORIDE
4-(3-(DiMethylaMino)propylcarbaMoyl)phenylboronic acid, pinacol ester
4-Aminophenylacetic acid, 98%
3,5-Dimethoxy-4-hydroxyphenylacetic acid
(2R)-2-(6-Methoxynaphth-2-yl)propanoic acid
9-Aminoacridine hemihydrate, 98%