TG HAVSMC Cell：人主动脉血管平滑肌传代贴壁细胞系 货号：GD-C6620572
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-(4-Fluorophenyl)-1-isopropylindole, 98%
Stainless Steel powder
2,3-Dihydro-5-furylboronic acid
4-Chloro-8-Methylquinoline
n-Butyl vinyl ether
4-(4-Methylpiperazino)aniline
Ethyl 4-chloro-2-Methylthio-5-pyriMidinecarboxylate
Trimethylphosphate, min. 97%
4-Chlorophenoxyacetyl chloride, tech., 90%
Cyclopropyl(phenyl)methanamine
1-(Pyridin-4-ylmethyl)-1H-pyrazole-4-boronic acid,pinacol ester
3-(3-PYRIDYL)BENZALDEHYDE
1H-Benzimidazole-5-carboxylic acid
N,N,N',N'-Tetraphenylbenzidine
3-Nitro-2-pyridinol
4-Iodobenzoic acid
2,4-Diphenyl-4-Methyl-1-pentene
5-BroMo-2-(1H-pyrazol-1-yl)pyridine
StrontiuM chloride, 99.99%, (trace Metal basis), anhydrous
Menadione, Powder, USP
2-(EthylMethylaMino)pyridine
Chlorfenvinphos
Methyltaurinesodium salt
2-ethoxyethyl chloroformate
Maleic acid
2-Bromo-4-(trifluoromethyl)pyridine
1,5-DICHLOROANTHRAQUINONE
Methyl Indole-5-Carboxylate
Isopropylidene Malonate, 98%
Pyruvic aldehyde diMethyl acetal, 97+%
DBE-5 dibasic ester
4-Fluoro-3-(trifluoroMethyl)phenol
2-BroMo-6-chloro-4-nitroanisole
tert-Butyl acetate
L-GLUCOSE
1-ETHYL-3-METHYLIMIDAZOLIUM DICYANAMIDE
L-Leucine ,99%
4-(1,3-Dithiolan-2-yl)benzene-1-carbothioamide, 97%
N-Benzhydryl-N-methylamine
[1-(N-Boc-aminomethyl)-cyclohexyl]-acetic acid
2-CHLOROCINNAMOYL CHLORIDE
Dibenzofuran-3-sulfonyl chloride
1,2,3-TRIPHENYLGUANIDINE
SULFANITRAN
3-Chloro-4-fluorobenzoic acid
TAPS sodiuM salt
p-Tolylsulfur Pentafluoride
2-Thioxotetrahydro-1,3-oxazole, 98%
7-Hydroxy-4-(trifluoroMethyl)-2H-chroMen-2-one, 97%
3,5-DINITROANILINE
SodiuM 1,2-Naphthoquinone-2-diazido-4-sulfonate
5,7-Dichloro-8-quinaldinol
Ethanone, 2-bromo-1-(2-iodophenyl)-
Benzothiazole-2-acetonitrile
5-hydroxy-4-methoxy-2-nitrobenzoic acid
(R)-(-)-2-AMINOHEPTANE
5-Chloro-1-MethyliMidazole, 98%
2,4,5-TriMethylbenzonitrile
TetrabroMo-o-sulfobenzoic Anhydride , 98%
5'-INOSINIC ACID BARIUM SALT
(4-Fluorophenyl)thiourea 98%
2,4-Dibromo-2,4-dimethyl-3-pentanone, 98%
4-Methylumbelliferylphosphate disodium salt trihydrate, 99%
Allyl 2,3,4,6-tetra-O-acetyl-β-D-glucopyranoside ,98%
Diglycolic acid
2,6-Dimethoxypyridine
Rubidium nitrate
4'-Ethoxyacetophenone
1-(3,5-Dichlorophenyl)-2-hydroxyethylaMine
Dithizone Silver CoMplex
4-BroMo-5-(4-Methoxyphenyl)pyrazole
PotassiuM chlorate, 99+%, ACS reagent
FuMonisin B2
3-Chloropivaloyl Chloride
5-Methyl-1H-benzotriazole
S-Benzyl-L-cysteine
2-Chloroacetophenone
2-Butanone
NO ELISA Kit
IL22 ELISA Kit
Human Tumor necrosis factor β，TNF-β ELISA Kit
TGL ELISA Kit
GLβ ELISA Kit
CD320 ELISA Kit
Monkey IL-10 (Interleukin-10) ELISA Kit
Human heat-labile enterotoxin B subunit,LTB ELISA Kit
Human ACA-IgM ELISA Kit
NT-4 ELISA Kit
Rappaport-Vassiliadis R10 broth
Tryptone Glucose Yeast Broth Medium
Triethyl orthopropionate, 98%
SodiuM perborate tetrahydrate, 97%
5-Hexen-1-ol, 98%
2-Methylnonane, 98+%
Chlorfenvinphos
Butanoic acid, 3-oxo-, 2-methylpropyl ester
Solvent blue 4 (C.I. 44045:1)
pteroic acid
DIBROMOBAPTA
(1'S, 3S)-1-(1'-PHENYLETHYL)-5-OXO-3-PYRROLIDINE CARBOXYLIC ACID
Delta-Nonalactone
Allyl phenyl ether, 99%
N-Boc-DL-proline, 98%
1-Butyl-1-MethylpyrrolidiniuM Chloride
PSS-Octavinyl substituted
3-Carboxy-4-Methylphenylboronic acid
6-BroMoquinoline, HCl
2-NAPHTHYL LAURATE
CHLOROMETHYL BUTYRATE
Dibutyltin diacetate, pract.
Diethyl phosphonoacetaldehyde diethyl acetal, tech., 90%
Cadmium standard solution, 1 mg/ml Cd in 0.5N HNO3, for AAS
Benzophenone-2,4'-dicarboxylic Acid Monohydrate
