ST2/o Cell：小鼠杂交瘤传代贴壁细胞系 货号：GD-C6620565
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Benzoylacetone, 98+%
Methyl 3-Methoxy-4-Methylbenzoate
4-NITROPHENYLOXAMIC ACID
2-Benzyloxy-5-broMopyriMidine, 95%
1,2-DIMETHYL-3-PROPYLIMIDAZOLIUM IODIDE
2,4-Dinitro-1-(trifluoroMethoxy)benzene
MAGNESIUM IODIDE
Acid Green 9
Chloro(pentamethylcyclopentadienyl)ruthenium(II) tetramer, min. 95%
Lithium acetate dihydrate, 98%
3-Iodo-5-methyl-1H-pyrrolo[2,3-b]pyridine
Managanese bromide
2,3-Dimethoxybenzaldehyde
4-Aminophthalimide
2,5-dioxo-1,4-cyclohexanedicarboxylic acid dimethyl ester
3-Methoxyphenylglyoxal hydrate
3,4-Dichlorobenzotrichloride
2-(4-BroMopyrazol-1-yl)benzonitrile
Stearic acid, 97%
Mefloquine Hydrochloride, USP
(-)-1,4-Di-O-benzyl-L-threitol
gibberellin A4
Sudan Yellow 3G = 3-Methyl-1-phenyl-4-(phenylazo)-pyrapl-5-ol
sym-Diformylhydrazine
1,2-Ethanedisulfonic acid
Benzoyl peroxide
FLUORESCEIN-5-MALEIMIDE
N-[3-(DiMethylaMino)Propyl]AcrylaMide (Stabilized With Mehq)
3,5-Dinitro-L-tyrosine, 98%
(3,3,3-Trifluoropropyl)trichlorosilane, 97%
PalMatine
Nα-p-Tosyl-L-arginine Methyl ester hydrochloride
4-BroMo-3,6-dihydro-2H-pyran
tert-Butyl N-(4-aMinobutyl)carbaMate
2-(4-METHOXYPHENOXY)ETHANETHIOAMIDE
POTASSIUM SULFAMATE
4-Piperidin-4-ylpiperidine, N-BOC protected
2,1,3-benzothiadiazol-5-amine(SALTDATA: FREE)
2-Aminoadenosine
Potassium 4-chlorophenyltrifluoroborate, 97%
TETRAMETHYLGERMANE
2,3-DIMETHYLBENZAMIDE
L-4-Nitrophenylalanine methyl ester hydrochloride
Mesna
2-Morpholino-1,3-thiazole-5-carboxylic acid
LincoMycin hydrochloride
L-Proline tert-Butyl Ester Hydrochloride
1,1,1,3,3,3-HexaMethyldisilazane, 98%, AcroSeal
4-[3-(TrifluoroMethyl)-1H-pyrazol-1-yl]benzoic acid, 97%
L-Aspartic acid 4-benzyl ester
2-(9-Oxoxanthen-2-yl)propionic Acid 1,5,7-Triazabicyclo[4.4.0]dec-5-ene Salt
prednisolone
Mercaptoacetic acid calcium salt trihydrate
3-Chloro-5-fluorobenzoic acid
coenzyme B12
(S)-N-Boc-4-Cyanophenylalanine
SodiuM carbonate decahydrate
4-(1-BROMOETHYL)BENZOIC ACID
2-FLUOROPHENETHYLAMINE
3-BroMo-4-chlorobenzonitrile
2-Methyl-2-propen-1-ol, 98%
5-Bromo-3-chloropyridine-2-carboxylic acid
2-Phenylbutyramide, 98%
5-methyl-1H-imidazole-4-carboxylic acid(SALTDATA: FREE)
1-BROMO-4-N-HEPTYLBENZENE
ETHYL COUMARIN-3-CARBOXYLATE
5-Chloroindoline
O-Acetyl-L-carnitine hydrochloride
1-(2-Phenoxyphenyl)MethanaMine hydrochloride
4,4'-Diacetoxybiphenyl
3-(4-BroMophenyl)-5-ethyl-1,2,4-oxadiazole
SodiuM bis(2-Methoxyethoxy)aluMiniuM hydride, 70 wt% sol. in toluene (ca. 3.5M)
LithiuM Metaborate, Reagent, ACS
4-AMinophenylsulfur Pentafluoride
1-bromo-2-methoxy-4-(trifluoromethyl)benzene
Benzenamine, 2-chloro-3-nitro-
2,5-Diaminotoluene sulfate
di(morpholin-4-yl) disulphide
IAPP ELISA Kit
Mouse Interleukin 23,IL-23 ELISA Kit
TNFsR-I ELISA Kit
Human primary hepatic carcinoma,PHC ELISA Kit
IFNα ELISA Kit
Rat Cluster of differentiation 30,CD30 ELISA Kit
Monkey β2-microglobulin,BMG/β2-MG ELISA Kit
ADMA ELISA Kit
Human CLCF1 (Cardiotrophin Like Cytokine Factor 1) ELISA Kit
NSE ELISA Kit
Xylose lysine Desoxycholate Agar
Mannitol-Egg-Yolk-Polymyxin agar Base
TriMethyl Orthobutyrate
Barium peroxide
5-Methyl-1H-Pyrrole-2-Carboxylic Acid Ethyl Ester
Ethyl 2-Methylthiazole-4-carboxylate, 98%
gibberellin A4
Potassium Sulphate
Ethanephosphonic acid
4-Chloro-3,5-dinitrobenzoic acid
DIRHODIUM (II) TETRAKIS(METHYL 2-PYRROLIDONE-5(S)-CARBOXYLATE)ACETONITRILE/2-PROPANOL COMPLEX
Trimethoprim
1,3-DiMethyl-5-Pyrazolone
4-Methylthiazole-5-carboxylic acid, 97%
2-ThiopheneMethanol, 97%
4-(Methylthio)benzyl alcohol
Diacetin
TIN (II) METHOXIDE
3-Nitro-2-pyridinethiol
AMMoniuM diMolybdate
2-(Thiophen-2-yl)-1,3,2-dioxaborinane
2-Chloro-6-fluoroaniline 97%
(2S,4S)-4-Amino-1-(tert-butoxycarbonyl)-pyrrolidine-2-carboxylic acid
(1R,2S,5R)-2-Isopropyl-5-methylcyclohexyl 2,2-Dihydroxyacetate
Boc-(S)-3-amino-4-(4-nitrophenyl)-butyric acid
