CNE-1 Cell：人鼻咽癌复苏传代细胞系 货号：GD-C6519246
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
6-BroMooxindole, 97%
2-Nitro-4-(trifluoroMethyl)phenol
(2R,3R,5S,6S)-5,6-Bis(hydroxyMethyl)-2,3-diMethoxy-2,3-diMethyl-1,4-dioxane
5-BroMoquinoline
LIGNOCERIC ACID ETHYL ESTER
N-HEXANESULFONYLACETONITRILE
PYRIDINE-2-THIOAMIDE
2-Methylpiperidine, 99%
3-Chloro-2,6-difluorobenzylamine
2-Methyl-3-phenyl-2-propen-1-ol
Oxaliplatin
1,2-BIS(TRIMETHYLSILYLOXY)CYCLOBUTENE
TRIETHANOLAMINE SALICYLATE
cis-11-Eicosenoic acid
2-Chloroquinoline
Cholic acid
5,6-Difluoro-o-anisaldehyde
3-BroMo-4-chlorobenzaldehyde
Zinc nitrate hexahydrate, 98%, extra pure
s-Trioxane
Azoic Diazo CoMponent 48
2,1-Benzisoxazole-3-carboxylic acid
D-Alanine, ethyl ester,hydrochloride (1:1)
N-Carbobenzyloxy-L-aspartic acid
4-Fluorophenylacetyl chloride
(R)-(-)-1-Amino-2-propanol
Hydroxypropyl-beta-cyclodextrin
2,3-Difluoroaniline
SodiuM pyruvate, 99%
4,5-DiMethylthiazole, 98%
1-Acetyl-3-ethylaMinopyrrolidine, 99%
2,5-Dichlorophenylboronic acid
N-tert-ButyltriMethylsilylaMine
OctylaMine
1-N-Boc-AMinocyclopentanecarboxylic acid
1,2-DICHLOROOCTAFLUOROCYCLOHEX-1-ENE
LEAD CARBONATE
6-Chloro-2,4-difluoroaniline, 97%
Patent Blue V, sodium salt
4-Aminobenzamidine hydrochloride
5-alpha-Androst-16-en-3-alpha-ol
1,1,1,3,3,3-Hexafluoro-2-propanol
1-(2,4-DICHLOROPHENYL)-2-(1H-IMIDAZOLE-1-YL) ETHANONE
N-Methylformanilide
o-Anisoyl chloride
YttriuM(III) nitrate hexahydrate
DisodiuM DiMercaptoMaleonitrile
(4-BroMo-3-Methylphenylcarbonyl)pyrrolidine
p-Terphenyl, 99+%, pure
HistaMine Phosphate, USP
2-AMino-2-oxazoline Hydrochloride
7-Azaindole
1-Phenyloxindole
Calcium oxalate
diisodecyl phthalate
ETHYL 2-(2-FORMYLAMINO-1,3-THIAZOL-4-YL)-2-OXOACETATE
MESO-2,3-DIMETHYLSUCCINIC ACID
2-Chloro-5-(trifluoroMethyl)phenylboronic acid
4-BROMO-3-METHYLBENZOYL CHLORIDE
2-Chloro-4,5-difluorobenzenesulfonyl chloride
Tri-n-butylphosphite, min. 94%
1,4-Di(2-pyridyl)
2-chloro-N,N,5-trimethylpyrimidin-4-amine(SALTDATA: FREE)
2-Hydroxy-6-methyl-5-nitropyridine
Methyldopa
2,3-DIMETHYLPHENOXYACETIC ACID
MagnesiuM Chloride (AS)
2-BroMothiazole
1-BroMo-4-chloro-2-fluorobenzene
5-[2-(Methylthio)ethyl]hydantoin
N-Cyclopropylthiourea
Benzyl 4-isocyanatotetrahydro-1(2H)-pyridinecarboxylate, 97%
Crystal Violet
Hydantoin
4-(4-nitrophenyl)morpholine
2-chloro-4-methoxy-1-nitrobenzene
Collodion
1,2,3,4,6-penta-O-acetyl-alpha-D-galactopyranose
RLT ELISA Kit
S100A10 ELISA Kit
Human PCDHβ2 ELISA Kit
Human FNTβ ELISA Kit
IFN-α ELISA Kit
Rat β2-microglobulin,BMG/β2-MG ELISA Kit
Chicken MIP-1β (Macrophage Inflammatory Protein 1 Beta) ELISA Kit
Human CCKBR ELISA Kit
PFN ELISA Kit
ALDH18A1 ELISA Kit
Alkaline Peptone Water
Halophilic Bacteria Selective Agar
4'-Chloroacetoacetanilide
Pyridine hydrofluoride
5,6'-DibroMo-3,3'-bipyridine, 90+%
2-Fluoro-4,4'-bis(trans-4-propylcyclohexyl)biphenyl, 97%
2,1-Benzisoxazole-3-carboxylic acid
Lithium deuteride
ginsenoside rb2
Cyclopentadecanone
3-FLUORO-DL-PHENYLALANINE
4-Fluoro-2-nitrotoluene
2-ButhyliMidazole
DL-Panthenol, 99%
2-Chloro-4-hydroxybenzaldehyde, 97%
4-(AMinoMethyl)benzoic acid
TrisodiuM triMetaphosphate
BenzyltriphenylphosphoniuM chloride
(S)-2-Vinyloxirane
4,4′-Dipyridyl N,N′-dioxide hydrate
CITRONELLIC ACID
L-Threonine methyl ester hydrochloride, 98+%
Dipyrrolidino(N-succinimidyloxy)carbeniumhexafluorophosphate
2-(2-Pyridinyl)-4-(trifluoromethyl)-5-pyrimidinecarboxylic acid ,95%
2,5-Dichloroisonicotinaldehyde
