RASMC Cell：大鼠主动脉平滑肌复苏传代细胞系 货号：GD-C6518763
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
6-Methoxybenzothiazole-2-carbonitrile, 99%
1,2-Cyclohexanedinitrilotetraacetic acid
4,5-Dichloro-2-n-octyl-4-isothiazolin-3-one
4-(trans-4-Ethylcyclohexyl)benzonitrile , 98%
2-PiperidinopyriMidine-5-boronic acid
2-Fluoro-5-Methylbenzenesulfonyl chloride, 97%
2-Chloro-4-iodobenzoic acid
3-Bromo-2-fluorobenzonitrile 99%
3-(Triethoxysilyl)propyl Methacrylate
1-Ethyl-piperidine-3-carboxylic acid
Cyclopropyl Phenyl Ketone
2,2,6,6-Tetramethylpiperidine
3-CHLORO-5-FLUOROANISOLE
TRI-N-BUTYLTIN ACETATE
Diethyl cyanomethylphosphonate
BenzyltributylaMMoniuM broMide
DihydroergotaMine Mesylate
2-BroMothiazole-4-Methanol
tert-Butyl broMoacetate, 99%
MolybdenuM Trioxide, Reagent, ACS
3'-BroMo-4'-fluoroacetophenone
2-Methyl-2-propen-1-ol
5-(4-methoxyphenyl)-5-oxopentanoic acid
1-chloro-2-iodo-4-(trifluoromethyl)benzene
7-anilino-4-hydroxynaphthalene-2-sulphonic acid
4-BROMO-3-PHENYL-1H-PYRAZOL-5-AMINE
Betaine
2-Chloro-3-(TrifluoroMethyl)Pyridine
cis,cis-1,5-Cyclooctadiene, 99%, stab. with 50-200ppM Irganox 1076
1-Chlorohexane, 98+%
9-Hydroxyfluorene
Triphenylphosphine hydrobroMide
DIPHENYLCARBAZONE
STRONTIUM STANNATE
α-Hydroxyhippuric acid
2',4',5'-TRIMETHYLACETOPHENONE
3-(1H-Pyrrol-1-yl)benzaldehyde
Boc-3-methyl-L-phenylalanine
1,10-Diacetoxydecane
3-Cyanophenyl isothiocyanate
2,4,6-TRIMETHOXYPYRIMIDINE
Dimethyl 2-hydroxyethylphosphonate
1-NITROPYRAZOLE
Acetylcholine broMide;β-Acetoxyethyl triMethyl aMMoniuM broMide;2-(Acetyloxy)-N,N,N-triMethyl ethanaMiniuM broMide
Divinylbenzene (M- and p- Mixture) (contains Ethylvinylbenzene, Diethylbenzene) (stabilized with TBC)
2-Dicyclohexylphosphino-2′,6′-diMethoxybiphenyl
2'-O-Methylguanosine Hydrate
FMoc-l-4-azidophe
Methyl 3-isothiocyanatothiophene-2-carboxylate, 97%
LYSOZYME
NeopentylaMine
HYDROXY-P-TOLYL-ACETIC ACID
2-chloro-6-methoxy-3-nitropyridine
Zirconium(IV)nitrate
H-Glu(OtBu)-OMe . HCl
Chlorogenic acid
Ethyl 2-(t-butoxycarbonylaMino)oxazole-5-carboxylate
BIS(4-NITROPHENYL)PHOSPHORIC ACID SODIUM SALT
2-(CHLOROMETHYL)TETRAHYDROPYRAN
2-Amino-4,6-dichloropyrimidine, tech., 95%
5-Formyl-2-furancarboxylic Acid
Fmoc-N-methylaminobenzoic acid
4-(Methylamino)benzonitrile
Sodium anthraquinone-2-sulfonate
4-Methyl-1-pentanol
BIS(TRIPHENYLPHOSPHINE)NICKEL(II) BROMIDE
4-Hexanolide
1-(3,5-Dichlorophenyl)-1H-pyrrole-2-carbaldehyde
1,10-Bis(acryloyloxy)decane (stabilized with MEHQ)
2-(CyclopropylaMinoMethyl)-5-fluorophenylboronic acid, pinacol ester
cis-2-AMino-1-cyclopentanecarboxylic acid, 98+%
3-(1,1,2,3,3,3-Hexafluoropropyl)adaMantane-1-carboxylic acid, 97%
2,6-Di-tert-butyl-4-hydroxyMethylphenol
N,N'-Bis(2-chloroethyl)oxaMide
Benzhydrylimine
4-(4-Bromo-benzoyl)-piperidine
2-(Allyldimethylsilyl)Pyridine
N-Cbz-N'-Boc-ethylenediamine
Porcine platelet activating factor,PAF ELISA Kit
IL-18BP ELISA Kit
NE ELISA Kit
Human galanin,GAL ELISA Kit
TRAIL-R3 ELISA Kit
CXCR4 ELISA Kit
Monkey αFP (Alpha-Fetoprotein) ELISA Kit
EXENDIN-4 ELISA Kit
Human STAT5 ELISA Kit
EAR3 ELISA Kit
BAT Agar
Dubos Broth Base
1-(2,5-diMethyl-4-(2,5-diMethylphenylazo)phenylazo)-2-naphthol
KIESSLGUHR
5-Fluoro-2'-deoxyuridine, 98+%
Methyl 2-Methylbutyrate, 98%
2-Methyl-2-propen-1-ol
silver dichromate
4,4'-methylenebis(2-methylcyclohexyl-amine)
1,8-Dinitroanthraquinone
3,4,5-Trichloronitrobenzene
2-Ethoxy-3-methylpyrazine
2-(2-DiethylaMinoethoxy)Ethanol
5-Methyl-1H-indazole-3-carboxylic acid, 97%
4-(tert-Butoxycarboxy)benzeneboronic acid, 97%
o-PhenylenediaMine, 98%
2-Benzothiazoleacetonitrile
3-(3-CYANO-PHENYL)-ACRYLIC ACID METHYL ESTER
Benzyl Methacrylate
Benzotriazole-1-yl-oxytripyrrolidinophosphoniuM hexafluorophosphate
1,4-Diiodobutane
PigMent Yellow 14
1-[5-(Trifluoromethyl)-2-pyridyl]piperidine-4-carboxylic acid , 95%
(5-Chloro-1H-indazol-3-yl)-methanol ,97%
L-Cysteine-glutathione disulphide
