HT-22 Cell：小鼠海马神经元复苏贴壁细胞系 货号：GD-C6420529
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Polyethylene glycol 4-tert-octylphenyl ether
2-Isopropoxyethanol
Rhodanine-3-propionic Acid , 98%
DipotassiuM hexachloropalladate
Bis(dibenzylideneacetone)palladiuM
DiMesitylaMMoniuM Pentafluorobenzenesulfonate , 98%
D-Fructose-1,6-diphosphate, trisodium salt octahydrate, 98%
2,5-Dichloroiodobenzene 98%
4-(1,3-Oxazol-5-yl)benzenesulfonyl chloride , 97%
4-Chlorobenzyl-2-chloro-4-(trifluoromethyl)pyrimidine-5-carboxylate 97%
3-Hydroxypropanesulfonic Acid (contains varying amounts of 3,3'-Oxydipropanesulfonic Acid) (ca. 80% in Water, ca. 7.8mol/L)
(R)-(+)-2-Phenylglycinol
Ethyl (2R,4R)-4-methyl-2-piperidinecarboxylate
S-[2-(Diethylamino)ethyl]O，O-diethylphosphorothioate
2-(4-Methylpiperazin-1-yl)benzaldehyde
4-Chlorobenzene-1-carbohydrazide
Desogestrel
Propargyl 4-broMobenzaMide
2-Methoxy-5-nitrophenol, 98%
TriaMterene, USP
2,6-Di-tert-butylnaphthalene
Bis(4-chlorobenzoic) anhydride
O-(tetrahydro-2H-pyran-2-yl)hydroxylamine
anthra[9,1,2-cde]benzo[rst]pentaphene-5,10-dione, chloro derivatives
4,4'-iminodianiline
CARBONYL CYANIDE 3-CHLOROPHENYLHYDRAZONE
PYRONIN Y
2,3-Difluoro-1-Propoxybenzene
N-AcetylMorpholine, 98%
1,2-DibroMobenzene, 98%
5-Norbornen-2-ol, Mixture of endo and exo
Nigericin sodiuM salt
4-Phenylazophenyl isothiocyanate, 97%
N-Methyl-2-(4-piperidinyloxy)acetaMide
N-METHYLBENZAMIDE
3-(4-Fluorophenoxy)propionitrile
Propargyl Bromide (80% in Toluene, ca. 9.2mol/L)
3-Aminosalicylic acid, 97%
4-Acetylpyrazole
(4-Aminomethyl)benzyl alcohol hydrochloride
3-HYDRAZINOBENZOIC ACID
Phenethyl cinnamate
6-Aminoquinoline
DL-2-AMinobutyric acid;DL-2-AMinobutanoic acid;DL-α-AMinobutanoic acid
1,6-Dichlorohexane
Hydrogen peroxide solution(30%)
2-Propylcyclohexanone
(2S,5S)-(+)-Hexanediol, 99%, 99% ee
2-BroMoMalonaldehyde, 99%
Benzyl alcohol
5-(4-Carboxyphenyl)-10,15,20-triphenylporphyrin
(4-Aminophenyl)piperidin-1-ylmethanone
1,3-dihydro-4(or 5)-methyl-2H-benzimidazole-2-thione, zinc salt
2-Chloro-6-methyl-5-nitropyridine
hexacarbonylchromium
Hexamethyldisilazane
trans-4-AMinocyclohexanol hydrochloride
2,2-DIMETHYL-5-THIOPHEN-3-YL-[1,3]DIOXANE-4,6-DIONE
3-METHYLCINNAMALDEHYDE
SodiuM peroxide
Hexyl Mercaptan
1-(Pyridin-2-yl)thiourea
2-{[5-(trifluoromethyl)-2-pyridinyl]amino}ethanol(SALTDATA: FREE)
6-Chloroquinazoline-2,4(1H,3H)-dione
N-(2,4-DINITROPHENYL)-L-ALANINE
1-Butaneboronic acid
2-Acetyl-3-methylpyrazine
3-PYRIDYLACETONITRILE
1-Fluoro-2-nitrobenzene
1,4-Dichloroanthraquinone
2-BroMo-1-(pyridin-2-yl)butan-1-one
Succinic anhydride, 99%
MeropeneM, USP
4-Methoxybutyl Acetate
stachydrine
2,5-Diaminopyridine
2-Nonanol
Dihexyl adipate
Renin ELISA Kit
Mouse L-Phenylalanine ammonla-lyase,PAL ELISA Kit
Human Helicobacter pylori IgM，Hp-IgM ELISA Kit
Human MRP1 ELISA Kit
apo-A1 ELISA Kit
Rat Interferon γ ,IFN-γ ELISA Kit
Chicken Hyaluronic acid,HA ELISA Kit
Human PKCγ (Protein Kinase C Gamma) ELISA Kit
PAF ELISA Kit
ADIPOR2 ELISA Kit
PH7.2 phosphate buffer (Medicine)
Tryptone Physiology Solution
DiMethyl vinylphosphonate, 98%
FORSKOLIN
4'-n-Octyloxybiphenyl-4-carboxylic acid, 99%
2-Iodobenzyl broMide, 96%
Bis(4-chlorobenzoic) anhydride
10-hydroxydec-2-enoic acid
(acetylacetonato)(1,5-cyclooctadiene)-rhodium(I)
Hexadecamethylheptasiloxane
3'-Bromo-4'-fluoroacetophenone
XYLOBIOSE
2,5-DibroMopyriMidine
4-BroMo-1H-indazole, 97+%
Ethyl potassiuM Malonate, 98%
1-Methyl-3-octyliMidazoliuM chloride
Boc-L-GlutaMic acid
1,3-Bis(Methoxycarbonyl)-2-Methyl-2-thiopseudourea
2-[2-(AMinoMethyl)phenylthio]benzyl alcohol
(1R,2R)-trans-2-AMinocyclopentanol hydrochloride
DL-PHENYLMERCAPTURIC ACID
Calcium carbonate, light powder, pure, 98%
(2S,4S)-1-tert-Butyl 2-methyl-4-hydroxypiperidine-1,2-dicarboxylate
3,5-Dimethyl-1-adamantanemethanol
alpha,alpha-Diphenylglycine, 98%
