SK-UT-1 Cell：人子宫平滑肌瘤复苏贴壁细胞系 货号：GD-C6418828
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
N-Boc-3-diMethylaMino-L-alanine, 97%
2,4-Dinitrotoluene solution
2,4-UNDECADIEN-1-OL
3,4,5-Trihydroxybenzaldehyde
4-[4-(2-Carboxybenzoyl)phenyl]butyric acid
N-PENTYL-4-AMINOBENZOATE
N,N-DiMethylglycine
2,5-Bis(trifluoromethyl)benzenesulphonyl chloride
2,2,5,5-Tetramethyl-4-phenyl-3-imidazoline-3-oxide-1-oxyl, free radical
Ethyl 3-chlorophenylacetate, 98+%
DL-Methioninol
5-FLUORO-2-METHYLPHENYL ISOTHIOCYANATE
2,4,6-Trichloropyrimidine
1-OCTANESULFONIC ACID, SODIUM SALT, MONOHYDRATE
4-BENZYLPIPERIDINE
2,2,2-TrifluoroethylaMine
DiundecylaMine
3-BroMo-2,4,6-triMethylpyridine
Tetraethylene glycol diMethyl ether, 99%
n-Butyric Anhydride
Dibutyltin Bis(trifluoroMethanesulfonate)
(E)-2-Buten-1-ol
3-methoxy-2-cyclopenten-1-one
iso-Propyl acrylate
Naphthalene-1-sulphonic acid sodium salt
2-Methylthiophene
8-BROMO-2-(TRIFLUOROMETHYL)QUINOLIN-4-OL
3,5-Dichloro-N-(3-fluorobenzyl)aniline, 97%
3-Chloro-5-fluorobenzoic acid, 97%
5-tert-Butyl-2-Methoxybenzoic acid, 98%
FMoc-Phe-OH-15N
2,3-Methylenedioxo-5-(Methoxycarbonyl)Methylphenylboronic acid, pinacol ester
BROMANILIC ACID
SODIUM CHOLATE HYDRATE, BIOCHEMIKA, >= 9
2,2,6,6-TETRAMETHYLPIMELIC ACID
Uracil-5-carboxylic acid Monohydrate, 98%
1,4'-bipiperidin-4-ol(SALTDATA: FREE)
Cyclobutane-1-carbonyl chloride , 97%
Methyl 3'-amino[1,1'-biphenyl]-3-carboxylate hydrochloride
3-Cyanopropyltriethoxysilane
3-(1H,1H,9H-HEXADECAFLUORONONYLOXY)-1,2-EPOXYPROPANE
4-FLUORO-3-METHYLBENZYLAMINE
Pimavanserin
4-METHOXYBENZYL MERCAPTAN
trans-3-FluorocinnaMic acid
Bis[2-(2-benzoxazolyl)phenolato]zinc(II)
2-Chloro-5-hydroxyMethylphenylboronic acid
Bis(4-broMophenyl) ether, 96%
1-Methyl-1H-iMidazole-4-carboxylic acid, 97%
1,2,3,4,5,6,7,8-Octahydrophenanthrene
Phenylthiohydantoin-glutaMic Acid
Ethyl 4-chloro-2-(trifluoromethyl)pyrimidine-5-carboxylate
1-Benzyl 3-methyl (3R)--1,3-dicarboxylate
(-)-N-boc-D-alpha-phenylglycinol
Pyrimidine, 5-bromo-2,4-dimethyl-
11-HYDROXYUNDECANOIC ACID
2-BroMo-6-phenylpyridine
COPPER(II) SULFATE HYDRATE, 98%
2-BroMo-5-Methoxybenzyl broMide
2-Chloro-6(MethylaMino)purine
Benzoyl chloride
2-(3,4-Dihydroxyphenyl)ethyl Alcohol
(S)-beta-(3-fluoro-phenyl)alanine
Chloromethyl-1,1,1,3,3,3-hexafluoroisopropyl ether
4,4'-Bipiperidine dihydrochloride
Acid Violet 49
1,2-Dibromo-4,5-difluorobenzene
Tetrahydro-2H-pyran-4-ylMethanol
1,2-Bis(chloroMethyl)-4,5-diMethylbenzene
DiMesitylfluoroborane
8-BroMo-6-chloroiMidazo[1,2-a]pyridine-3-carbaldehyde
Zinc selenide, 99.99%, (trace Metal basis)
StrontiuM Nitrate, Crystal, Reagent, ACS
3-Methyl-1-phenyl-5-pyrazolone
2,3-Quinoline dicarboxylic acid
4-chlorobutyl ether
1H-Indene-1,3(2H)-dione, 2-(4-nitrophenyl)-
3-(4-Fluorophenyl)propionic acid
Ang 2 ELISA Kit
IL-1 ELISA Kit
ACS ELISA Kit
Human CALU ELISA Kit
VCAM-1/CD106 ELISA Kit
FRAP1 ELISA Kit
Monkey cTn-I/TNNI3 (cardiac Troponin I) ELISA Kit
Human OGP (Osteogenic Growth Peptide) ELISA Kit
Human TS/TYMS (Thymidylate Synthetase) ELISA Kit
GP5 ELISA Kit
Free Biotin Assay Medium
Buffered Peptone Water
Indane, 95%
CI 37085
Isosorbide 5-mononitrate
2-Methyl-4-Nitropyridine
(E)-2-Buten-1-ol
Polytetrafluoro-Ethylene
[1,3]oxazolo[4,5-b]pyridine-2(3H)-thione
(-)-Episesamin
2,3-Dichlorophenylacetonitrile
2-(TERT-BUTYLDIMETHYLSILYL)-1,3-DITHIANE
4-Phenoxybenzonitrile
Ethyl 3-(Methylthio)propionate, 98%
2-Fluorobenzaldehyde, 97%
PolyacrylaMide
[D-Phe7]-Bradykinin acetate salt
N-Phenylglycine potassiuM salt
2,4-DICHLOROPHENOXYACETIC ACID HYDRAZIDE
2-BroMo-6-Methylpyridine
trans,trans,cis-1,5,9-Cyclododecatriene, 98+%
2-(4-Aminophenyl)ethanol
4-Methyl pyrrole-2-carboxylic acid
1-tert-Butyl-2-thiourea
Isosorbide, 98%
