MASMC Cell：小鼠气道平滑肌复苏贴壁细胞系 货号：GD-C6418337
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
如何进行细胞复苏：1)从液氮容器中取出冻存管，直接浸入37℃温水中，并不时摇动令其尽快融化；2)从37℃水浴中取出冻存管，打开盖子，用吸管吸出细胞悬液，加到离心管并滴加10倍以上培养液，混匀；3)离心，1000rpm，5min；4)弃去上清液，加入含10%小牛血清培养液重悬细胞，计数，调整细胞密度，接种培养瓶，37℃培养箱静置培养；5)次日更换一次培养液，继续培养。【温馨提醒】1)从增殖期到形成致密的单层细胞以前的培养细胞都可以用于冻存，但Z好为对数生长期细胞。在冻存前一天Z好换一次培养液；2)将冻存管放入液氮容器或从中取出时，要做好防护工作(戴棉手套)，以免冻伤；3)冻存和复苏Z好用新配制的培养液。细胞复苏的时候，有些初次实验员将冻存管从液氮中取出后，放在室温下，经常发生冻存管爆炸，该怎么避免出现这种情况吗？其中在拧紧冻存管的时候是否有哪些细节要注意的？一般常用的方法是取出冻存管后在超净工作台中用酒精棉球擦试管口，再稍拧松盖子，再放37度水浴锅中摇溶，在将细胞转移到装有37度预热过的培养基的试管中，迅速离心，再用培养基洗一遍。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-[n-Butyl(4-Methoxybenzyl)sulfaMoyl]benzeneboronic acid, 98%
4-Phenoxybutyric acid
alpha-Naphthyl Red hydrochloride
3-[(4-Chloro-3-Methylphenoxy)Methyl]phenylboronic acid
Ethyl 2-aMino-4-Methylthiazole-5-carboxylate
4-FORMYL-TRANS-STILBENE
N-Cyclohexyl 3-fluorobenzaMide
1-Ethyl-3-methylimidazolium trifluoromethanesulfonate
6-Chloro-7-hydroxy-4-(trifluoromethyl)coumarin
5-Phenylpenta-2,4-dienoic acid
Monomethyl Azelate
3-CHLORO-2-METHYLPHENYL METHYL SULFIDE
1-(4-Fluorophenyl)ethanol
Tetraoctylammonium bromide
1-Butyl-3-methylimidazolium tetrafluoroborate
(1-Ethoxyethylidene)Malononitrile
3-DiMethylaMino-2,2-diMethyl-1-propanol
3-BroMo-2-Methylanisole
PotassiuM nitrate, 99+%, for analysis
Glucose Syrup, FCC
PentafluoropropionaMide
deferoxamine mesylate
3-(Cyclohexylamino)propyltrimethoxysilane
Sodium sesquicarbonate
1,1,2,2-Tetrabromoethane
N,N,N',N'-1,4-PHENYLENEDIAMINETETRAACETIC ACID
D-2,4-Dichlorophenylalanine
2-MethylMalonaMide, 95%
Cyclopentene oxide, 97%
SodiuM aMMoniuM hydrogen phosphate tetrahydrate, Reagent Grade
SodiuM broMate
2-Methoxypyrazine
N-Ethyl-2-nitro-4-trifluoroMethylaniline
Valerophenone
4-FLUORO-2-METHOXYACETOPHENONE
BroMotrichloroMethane
5-Bromo-2-furaldehyde, 97%
6-methylimidazo[1,2-a]pyridine-2-carboxylic acid(SALTDATA: FREE)
2-Bromo-6-methylbenzoic acid
Piperitone
Vinflunine
4-Methylbenzenesulfonhydrazide
1,4-HEXADIENE
3-Hydroxy-2-nitropyridine
3-(Ethoxycarbonyl)phenylboronic acid (contains varying aMounts of Anhydride)
4-Chlorocatechol
4-Methoxycarbonylindole-2-boronic acid pinacol ester
Ethyl 1-broMocyclobutanecarboxylate, 95%
FuMaric acid Monoethyl ester, 95%
4-(TrifluoroMethoxy)benzyl BroMide
3-Pentanone
Tetrabromostearicacid
Pyridoxine hydrochloride
3,5-Dichloro-4-Pyridinecarbonitrile
Zolpidem Tartrate
8-(TRIFLUOROMETHYL)QUINOLIN-4-OL
SEC-BUTYL ETHYL ETHER
Isoquinoline-4-boronic acid
1,2,3,4-Tetrafluorobenzene
1-Chloro-2,4-dibromo-6-fluorobenzene 98%
2,4-Diaminotoluene, 98%
3',5'-Dihydroxyacetophenone
Dichlorohexylmethylsilane
2-Formylphenoxyacetic acid
2-Bromo-4-pyridinecarboxaldehyde
Ironpentacarbonyl
Cefadroxil
LeishMan stain
(1R,2R)-(-)-1-AMino-2-indanol
2-AMino-5-Methylphenyboronic acid, pinacol ester
4-Fluorophenol, 99%
TriMethylstannylMethyldiphenylsilan, 96%
N-Phthaloylglycine
1-Ethoxycarbonyl-4-piperidone
Tetrabutylammonium iodide
Enfuvirtide
Pyridine,2-(chloromethyl)-3,5-dimethyl-, hydrochloride (1:1)
ciprofibrate
Porcine transforming growth factor β1,TGF-β1 ELISA Kit
C1qR1 ELISA Kit
Human H3 ELISA Kit
Human GSTθ1 ELISA Kit
HIS ELISA Kit
DHVD3 ELISA Kit
PDGFA ELISA Kit
Human PABPC1L ELISA Kit
Human SOCS1 ELISA Kit
P13K ELISA Kit
Alkaline Peptone Water
Murashige & Skoog Macronutrients
sec-Butanol
Ethyl 2-Oxocyclopentanecarboxylate
5-Nitroindole
2-Hydroxy-6-Methyl-5-nitropyridine, 98%
deferoxamine mesylate
2-Hydroxy-3-nitropyridine
1-methyl-5-amine-1H-Tetrazole
2-sec-butyl-4,6-dinitrophenol
YTTRIUM(III) BROMIDE HYDRATE
1,3-Dicyanobenzene
DL-^a-Tocopherol, 97+%
4-Acetyl-2-chloropyridine, 97%
N-tert-Butyl-2-chlorobenzaMide, 97%
tert-Butyl hydroperoxide solution
4-BUTOXYACETANILIDE
CALCIUM NITRATE HYDRATE
PHENYLBORON DICHLORIDE
5-AMino-2-chloropyridine
Di-n-hexyl phthalate, 97%
Carbon disulfide, for spectroscopy, 99.9%
1-chloro-5-isocyanato-2,4-dimethoxybenzene(SALTDATA: FREE)
Beta-Lactamase
L-(+)-Cystathionine
