SZ95 Cell：人皮脂腺传代细胞系 货号：GD-C6218713
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-(TrifluoroMethyl)benzyl chloride, 98%
2-Isopropyl-5-Methyl-2-hexenal
TETRAPHENYLARSONIUM CHLORIDE HYDROCHLORIDE
4'-AMINOOCTANOPHENONE
1,1′-Binaphthyl-2,2′-diaMine
3-(DiMethylcarbaMoyl)-5-nitrophenylboronic acid
2-Hydroxy-5-nitropyridine
(pyrimidin-4-ylmethyl)amine(SALTDATA: 2HCl)
(S)-1-N-Boc--2-carboxylic acid
Copper(II) methoxide, 99%
5-methyl-1H-indazole(SALTDATA: FREE)
Ketoprofen
1-Iodopentane
Heptyl 4-hydroxybenzoate
DL-2,4-DIAMINOBUTYRIC ACID DIHYDROCHLORIDE
2-Thiopheneacetyl chloride
Difluoroacetaldehyde Ethyl HeMiacetal
(4-BroMopyridin-3-yl)Methanol
Sudan I, pure
MES, Monohydrate, Ultrapure
2-(BroMoMethyl)-4,4,5,5-tetraMethyl-1,3,2-dioxaborolane
Drostanolone Enanthate
acid violet 7
1-Chloroheptane
2-Methylundecanal
Sodium hydride
SODIUM METHACRYLATE
1,4-Dihydro-2,6-DiMethyl-3,5-Pyridinedicarboxylic Acid Diethyl Ester
4-Fluoronaphthalene-1-boronic acid, 98%
Nitroterephthalic acid, 99%
Astragaloside III
YttriuM nitrate hexahydrate
METHYL 4-(AMINOMETHYL)BENZOATE HYDROCHLORIDE
Terpineol
2′-AMinoacetophenone hydrochloride
2-PYRIDYLDITHIOCARBAMIC ACID ETHYL ESTER
2,4-Difluorophenyl isocyanate, 99%
4-Amino-2,6-dimethylpyrimidine
2-Bromoethyl methyl ether
o-Methylvalerolactim
L-Tyrosine
EUGENOL ACETATE
carbazol-4-one hydrochloride
(+/-)-trans-1,2-Diaminocyclohexane
Oleic acid
2-Naphthyl phosphate sodiuM salt
3,5,4'-TribroMosalicylanilide
1-Nitroso-2-naphthol-3,6-disulfonic acid, disodiuM salt hydrate, pure, indicator grade
3,5-Dichlorobenzene-1-carbohydrazide, 97%
8,8'-DIQUINOLYL DISULFIDE
Dibenzyl Phosphate
(3S,8S,9S,10R,13R,17R)-17-[(1R,4R)-4-ethyl-1,5-dimethyl-hexy...
5-Bromo-2-hydroxy-3-(trifluoromethyl)pyridine
2,4-dichlorobiphenyl
3-acetoxypyridine
CHROMONE-3-CARBOXYLIC ACID
GadoliniuM rod, 6.35MM (0.25in) dia, 99.9% (Metals basis excluding Ta)
N-METHYLSUCCINIMIDE
DexaMethasone
Diethyl(3-pyridyl)borane
3-Ethoxyphenethylamine
5-Formylthiophene-3-boronic acid
6-Chloro-1,2,3,4-tetrahydroquinoline
4-Carboxylphenylboronic acid pinacol ester
2-Acetyl-1-methylpyrrole
TEMAZEPAM
2,3-DiMethyl-1,3-butadiene;2,3-DiMethylerythrene;Diisopropenyl;β-γ-DiMethyl-Δ-α-γ-butadiene;
3-Methyl-5-(5-Methylisoxazol-3-yl)isoxazole-4-carboxylic acid
1-(4-BroMophenyl) Hydrochloride
6-Ethyl-1,6-dihydropyriMidin-5-ylboronic acid
Dichlorophenylborane, 97%
3-aMino-5-hydroxybenzoic acid hydrochloride hydrate, 95%
2-(4-DiethylaMino-2-hydroxybenzoyl)benzoic Acid
2,6-DiMethylbenzyl Chloride
Chlorocarbonyl ferrocene
tert-butyl 1,8-diazaspiro[5.5]undecane-1-carboxylate
3-CHLORO-5-FORMYL-2-METHOXYPYRIDINE
2,4-difluorobenzamide
OxLDL ELISA Kit
IL2 ELISA Kit
Human TNF-α ELISA Kit
Human DAG ELISA Kit
α1M ELISA Kit
TBAM ELISA Kit
Monkey Interleukin 6,IL-6 ELISA Kit
Human Brucella Ab IgG ELISA Kit
H-FABP ELISA Kit
GDF8 ELISA Kit
Muller–Kauffmann Tetrathionate Broth Base
BBL Agar Medium
Guaiazulene, 98+%
D-Fructose-1,6-diphoshate calcium salt
5-Cyclobutyl-5-phenylhydantoin, 97+%
2-MethyliMidazo[4,5-c]pyridine, 99%
Drostanolone Enanthate
Selenium
(6aR,12aR)-6a,12a-dihydro-6H-[1,3]dioxolo[5,6][1]benzofuro[3,2-c]chromen-3-yl beta-D-glucopyranoside
Benzoin
1H,1H,5H-OCTAFLUOROPENTYL ACRYLATE
4-Chloro-isatoic anhydride
3,4-Dihydroxybenzophenone, 98%
AluMinuM antiMonide, 99.99% (Metals basis)
3-AMidinopyridine hydrochloride, 97%
2'-DeoxythyMidine-5'-diphosphate trisodiuM salt
2-(ChloroMethyl)-5-nitrophenylboronic acid
7-Chloroquinoline-4-boronic acid
Oxacyclododecan-2-one
3-Iodobenzyl broMide
3-Methylbenzoyl chloride
Tribromoacetic acid, 99%
Methanol-d, 99.0 atom % D, for NMR
octahydropyrrolo[1,2-a]pyrazine(SALTDATA: FREE)
2-(4-Bromophenyl)benzothiazole
